All Outputs (57)

Molecular weight analysis of starches: which technique? (2016)
Journal Article
Harding, S. E., Adams, G. G., & Gillis, R. B. (2016). Molecular weight analysis of starches: which technique?. Starch/Stärke, 68(9-10), https://doi.org/10.1002/star.201600042

A number of methods are available to researchers for estimating molecular weights (molar masses) and molecular weight distributions of starches − or those in solution, using an appropriate solvent/solubilisation protocol. We outline the methods avail... Read More about Molecular weight analysis of starches: which technique?.

Aspects of the analytical ultracentrifuge determination of the molar mass distribution of polysaccharides (2016)
Book Chapter
Harding, S. E., Adams, G. G., Gillis, R. B., Almutairi, F. M., & Morris, G. A. (2016). Aspects of the analytical ultracentrifuge determination of the molar mass distribution of polysaccharides. In S. Uchiyama, F. Arisaka, W. F. Stafford, & T. M. Laue (Eds.), Analytical ultracentrifugation: instrumentation, software and applications (375-386). Springer. https://doi.org/10.1007/978-4-431-55985-6_18

Molar mass or ‘molecular weight’ is one of the most fundamental parameters describing a macromolecule. Because of their polydisperse nature, polysaccharides are usually described by distributions of molar mass. SEC-MALS (size exclusion chromatography... Read More about Aspects of the analytical ultracentrifuge determination of the molar mass distribution of polysaccharides.

A glycoconjugate of Haemophilus influenzae Type b capsular polysaccharide with tetanus toxoid protein: hydrodynamic properties mainly influenced by the carbohydrate (2016)
Journal Article
Abdelhameed, A. S., Adams, G. G., Morris, G. A., Almutairi, F. M., Duvivier, P., Conrath, K., & Harding, S. E. (2016). A glycoconjugate of Haemophilus influenzae Type b capsular polysaccharide with tetanus toxoid protein: hydrodynamic properties mainly influenced by the carbohydrate. Scientific Reports, 6(1), Article 22208. https://doi.org/10.1038/srep22208

Three important physical properties which may affect the performance of glycoconjugate vaccines against serious disease are molar mass (molecular weight), heterogeneity (polydispersity), and conformational flexibility in solution. The dilute solution... Read More about A glycoconjugate of Haemophilus influenzae Type b capsular polysaccharide with tetanus toxoid protein: hydrodynamic properties mainly influenced by the carbohydrate.

Coiled coil type neoglycoproteins presenting three lactose residues (2016)
Journal Article
Sweeney, S. M., Bullen, G. A., Gillis, R. B., Adams, G. G., Rowe, A. J., Harding, S. E., Tucker, J. H., Peacock, A. F., & Murphy, P. V. (2016). Coiled coil type neoglycoproteins presenting three lactose residues. Tetrahedron Letters, 57(13), https://doi.org/10.1016/j.tetlet.2016.02.005

Scaffold design, synthesis and application are relevant for biomedical research. For example, multivalent interactions, such as those between cell surface glycoproteins and lectins can influence the potency and duration of signalling. The spacing bet... Read More about Coiled coil type neoglycoproteins presenting three lactose residues.

Location dependent coordination chemistry and MRI relaxivity, in de novo designed lanthanide coiled coils (2015)
Journal Article
Berwick, M. R., Slope, L. N., Smith, C. F., King, S. M., Newton, S. L., Gillis, R. B., Adams, G. G., Rowe, A. J., Harding, S. E., Britton, M. M., & Peacock, A. F. A. (in press). Location dependent coordination chemistry and MRI relaxivity, in de novo designed lanthanide coiled coils. Chemical Science, 7(3), https://doi.org/10.1039/c5sc04101e

Herein, we establish for the first time the design principles for lanthanide coordination within coiled coils, and the important consequences of binding site translation. By interrogating design requirements and by systematically translating binding... Read More about Location dependent coordination chemistry and MRI relaxivity, in de novo designed lanthanide coiled coils.

The effectiveness of information-motivation-behavioural skills model-based diabetes self-management education among patients with type 2 diabetes in Jordan (IMB-DSME): trial protocol (2015)
Presentation / Conference Contribution
Albelbisi, Z., Adams, G. G., Windle, R., & Blake, H. The effectiveness of information-motivation-behavioural skills model-based diabetes self-management education among patients with type 2 diabetes in Jordan (IMB-DSME): trial protocol. Presented at 2015 11th UK Society for Behavioural Medicine Annual Scientific Meeting

Abstract for UKSBM conference:
Background: According to the World Health Organization in 2014, 9% of adults over 18 years old are diagnosed with diabetes and more than 1.5 million deaths occur directly by diabetes in low and middle-income countries.... Read More about The effectiveness of information-motivation-behavioural skills model-based diabetes self-management education among patients with type 2 diabetes in Jordan (IMB-DSME): trial protocol.

Application of recent advances in hydrodynamic methods for characterising mucins in solution (2015)
Journal Article
Almutairi, F. M., Cifre, J.-G. H., Adams, G. G., Kök, M. S., Mackie, A. R., de la Torre, J. G., & Harding, S. E. (2016). Application of recent advances in hydrodynamic methods for characterising mucins in solution. European Biophysics Journal, 45(1), 45-54. https://doi.org/10.1007/s00249-015-1075-0

Mucins are the primary macromolecular component of mucus—nature’s natural lubricant—although they are poorly characterised heterogeneous substances. Recent advances in hydrodynamic methodology now offer the opportunity for gaining a better understand... Read More about Application of recent advances in hydrodynamic methods for characterising mucins in solution.

Ultracentrifuge Methods for the Analysis of Polysaccharides, Glycoconjugates, and Lignins (2015)
Book Chapter
Harding, S. E., Adams, G. G., Almutairi, F., Alzahrani, Q., Erten, T., Samil Kök, M., & Gillis, R. B. (2015). Ultracentrifuge Methods for the Analysis of Polysaccharides, Glycoconjugates, and Lignins. In Analytical Ultracentrifugation (391-439). Academic Press. https://doi.org/10.1016/bs.mie.2015.06.043

Although like proteins, polysaccharides are synthesized by enzymes, unlike proteins there is no template. This means that they are polydisperse, do not generally have compact folded structures, and are often very large with greater nonideality behavi... Read More about Ultracentrifuge Methods for the Analysis of Polysaccharides, Glycoconjugates, and Lignins.

The effectiveness of Information- Motivation-Behavioural skills model-based Diabetes Self-Management Education among patients with type 2 diabetes in Jordan (IMB-DSME): trial protocol (2015)
Presentation / Conference Contribution
Albelbisi, Z., Adams, G., Windle, R., & Blake, H. (2015, August). The effectiveness of Information- Motivation-Behavioural skills model-based Diabetes Self-Management Education among patients with type 2 diabetes in Jordan (IMB-DSME): trial protocol. Paper presented at American Association of Diabetes Educators Annual Meeting, New Orleans, USA

Matrix-free hydrodynamic study on the size distribution and conformation of three technical lignins from wood and non-wood (2015)
Journal Article
Alzahrani, Q. E., Adams, G. G., Gillis, R. B., Besong, T. M., Kök, M. S., Fong, E., Harding, R. A., van Dam, J. E., Gosselink, R. J., Rowe, A. J., & Harding, S. E. (2016). Matrix-free hydrodynamic study on the size distribution and conformation of three technical lignins from wood and non-wood. Holzforschung, 70(2), https://doi.org/10.1515/hf-2014-0318

Molecular weight (MW) and related conformational data of three commercially available technical lignins (Alcell L, kraft L, and soda L) have been studied by means of analytical ultracentrifugation, taking advantage of some recent developments in both... Read More about Matrix-free hydrodynamic study on the size distribution and conformation of three technical lignins from wood and non-wood.

Recent advances in the analysis of macromolecular interactions using the matrix-free method of sedimentation in the analytical ultracentrifuge (2015)
Journal Article
Harding, S., Gillis, R., Almutairi, F., Erten, T., Kök, M., & Adams, G. (2015). Recent advances in the analysis of macromolecular interactions using the matrix-free method of sedimentation in the analytical ultracentrifuge. Biology, 4(1), https://doi.org/10.3390/biology4010237

Sedimentation in the analytical ultracentrifuge is a matrix free solution technique with no immobilisation, columns, or membranes required and can be used to study self-association and complex or “hetero”-interactions, stoichiometry, reversibility an... Read More about Recent advances in the analysis of macromolecular interactions using the matrix-free method of sedimentation in the analytical ultracentrifuge.

Characterization of capsular polysaccharides and their glycoconjugates by hydrodynamic methods (2015)
Book Chapter
Harding, S. E., Abdelhameed, A. S., Gillis, R. B., Morris, G. A., & Adams, G. G. (2015). Characterization of capsular polysaccharides and their glycoconjugates by hydrodynamic methods. In Carbohydrate-based vaccines (211-227). Springer. https://doi.org/10.1007/978-1-4939-2874-3_13

Hydrodynamic methods are relevant for the characterization of carbohydrates such as capsular bacterial polysaccharides or glycoconjugates in solution. This chapter focuses on the following hydrodynamic methods: sedimentation velocity analytical ultra... Read More about Characterization of capsular polysaccharides and their glycoconjugates by hydrodynamic methods.

Hydrodynamic characterisation of chitosan and its interaction with two polyanions: DNA and xanthan (2014)
Journal Article
Almutairi, F. M., Erten, T., Adams, G. G., Hayes, M., McLoughlin, P., Kök, M. Ş., Mackie, A. R., Rowe, A. J., & Harding, S. E. (2015). Hydrodynamic characterisation of chitosan and its interaction with two polyanions: DNA and xanthan. Carbohydrate Polymers, 122, https://doi.org/10.1016/j.carbpol.2014.09.090

Chitosan, a soluble polycationic derivative of insoluble chitin, has been widely considered for use in the food, cosmetic and pharmaceutical industries. Commercial (“C”) and in-house laboratory (“L”) prepared chitosan samples extracted from crustaceo... Read More about Hydrodynamic characterisation of chitosan and its interaction with two polyanions: DNA and xanthan.

On hydrodynamic methods for the analysis of the sizes and shapes of polysaccharides in dilute solution: a short review (2014)
Journal Article
Morris, G. A., Adams, G. G., & Harding, S. E. (2014). On hydrodynamic methods for the analysis of the sizes and shapes of polysaccharides in dilute solution: a short review. Food Hydrocolloids, 42, https://doi.org/10.1016/j.foodhyd.2014.04.014

Background: Polysaccharides and their derivatives are increasingly being used by the food, cosmetic and pharmaceutical industries: physical properties like size and conformation are important contributors to their performance.
Discussion: Here the u... Read More about On hydrodynamic methods for the analysis of the sizes and shapes of polysaccharides in dilute solution: a short review.

Comparative heterogeneity, molecular weights and viscosities of xanthans of different pyruvate and acetate content (2014)
Journal Article
Erten, T., Adams, G. G., Foster, T. J., & Harding, S. E. (2014). Comparative heterogeneity, molecular weights and viscosities of xanthans of different pyruvate and acetate content. Food Hydrocolloids, 42, https://doi.org/10.1016/j.foodhyd.2014.04.012

Introduction: The bacterial exopolysaccharide xanthan is a well-known hydrocolloid, with a high viscosity deriving from its large molecular weight and volume.
Materials and methods: Four different xanthans deriving from Xanthomanas campestris -two w... Read More about Comparative heterogeneity, molecular weights and viscosities of xanthans of different pyruvate and acetate content.

“Medioglycaemia”: a new concept in glycaemic control in intensive care (ICU) units? (2012)
Journal Article
Tomlinson, V. H., Langley, J., Meal, A. G., & Adams, G. G. (2012). “Medioglycaemia”: a new concept in glycaemic control in intensive care (ICU) units?. Journal of Diabetes Mellitus, 25(4), https://doi.org/10.4236/jdm.2012.24056

Introduction: Critically ill patients can experience stress-induced hyperglycaemia. Glycaemic control therapy (GCT) is administered to control patients’ blood glycaemic levels and reduce the incidence of infection, myocardial infarctions and organ fa... Read More about “Medioglycaemia”: a new concept in glycaemic control in intensive care (ICU) units?.

Diabetes health-beliefs, self-care practices and glycaemic control among Malaysian young adults with diabetes (2011)
Journal Article
Aris, A., Blake, H., & Adams, G. (2011). Diabetes health-beliefs, self-care practices and glycaemic control among Malaysian young adults with diabetes. Journal of Nursing Referência (Revista de Enfermagem Referência), 4(Supp),

Self-care practices and glycaemic control remain suboptimal among young adults with diabetes. One of the known factors to influence self-care behaviours is health beliefs. Targeting health beliefs through diabetes education facilitates individual’s d... Read More about Diabetes health-beliefs, self-care practices and glycaemic control among Malaysian young adults with diabetes.