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Ultracentrifuge methods for the analysis of polysaccharides, glycoconjugates, and lignins

Harding, Stephen E.; Adams, Gary G.; Almutairi, Fahad; Alzahrani, Qushmua; Erten, Tayyibe; Samil Kök, M.; Gillis, Richard B.


Gary G. Adams

Fahad Almutairi

Qushmua Alzahrani

Tayyibe Erten

M. Samil Kök

Richard B. Gillis


Although like proteins, polysaccharides are synthesized by enzymes, unlike proteins there is no template. This means that they are polydisperse, do not generally have compact folded structures, and are often very large with greater nonideality behavior in solution. This chapter considers the relevant analytical ultracentrifuge methodology available for characterizing these and related carbohydrate-based systems and information this methodology supplies, in terms of sizes, shapes, and interactions using a comprehensive range of examples, including glycoconjugates and lignins. The relevance and potential of recent software developments such as SEDFIT-MSTAR, the Extended Fujita algorithm, and HYDFIT are considered.


Harding, S. E., Adams, G. G., Almutairi, F., Alzahrani, Q., Erten, T., Samil Kök, M., & Gillis, R. B. (2015). Ultracentrifuge methods for the analysis of polysaccharides, glycoconjugates, and lignins. In Analytical UltracentrifugationAcademic Press. doi:10.1016/bs.mie.2015.06.043

Acceptance Date Aug 21, 2015
Online Publication Date Aug 21, 2015
Publication Date Sep 24, 2015
Deposit Date Apr 4, 2017
Electronic ISSN 766879
Peer Reviewed Peer Reviewed
Issue 562
Series Title Methods in Enzymology
Book Title Analytical Ultracentrifugation
ISBN 9780128029084
Keywords Size; Shape; Interactions; SEDFIT-MSTAR; Extended Fujita; Flexibility
Public URL
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Copyright Statement Copyright information regarding this work can be found at the following address:
Additional Information Series editor, James Cole.