Skip to main content

Research Repository

Advanced Search

Recent advances in the analysis of macromolecular interactions using the matrix-free method of sedimentation in the analytical ultracentrifuge

Harding, Stephen; Gillis, Richard; Almutairi, Fahad; Erten, Tayyibe; Kök, M.; Adams, Gary

Authors

Fahad Almutairi

Tayyibe Erten

M. Kök



Abstract

Sedimentation in the analytical ultracentrifuge is a matrix free solution technique with no immobilisation, columns, or membranes required and can be used to study self-association and complex or “hetero”-interactions, stoichiometry, reversibility and interaction strength of a wide variety of macromolecular types and across a very large dynamic range (dissociation constants from 10−12 M to 10−1 M). We extend an earlier review specifically highlighting advances in sedimentation velocity and sedimentation equilibrium in the analytical ultracentrifuge applied to protein interactions and mucoadhesion and to review recent applications in protein self-association (tetanus toxoid, agrin), protein-like carbohydrate association (aminocelluloses), carbohydrate-protein interactions (polysaccharide-gliadin), nucleic-acid protein (G-duplexes), nucleic acid-carbohydrate (DNA-chitosan) and finally carbohydrate-carbohydrate (xanthan-chitosan and a ternary polysaccharide complex) interactions.

Journal Article Type Article
Publication Date Mar 6, 2015
Journal Biology
Electronic ISSN 2079-7737
Publisher MDPI
Peer Reviewed Peer Reviewed
Volume 4
Issue 1
APA6 Citation Harding, S., Gillis, R., Almutairi, F., Erten, T., Kök, M., & Adams, G. (2015). Recent advances in the analysis of macromolecular interactions using the matrix-free method of sedimentation in the analytical ultracentrifuge. Biology, 4(1), https://doi.org/10.3390/biology4010237
DOI https://doi.org/10.3390/biology4010237
Keywords protein; carbohydrate; nucleic acid; interaction; hydrodynamics
Publisher URL http://www.mdpi.com/2079-7737/4/1/237
Copyright Statement Copyright information regarding this work can be found at the following address: http://creativecommons.org/licenses/by/4.0

Files

biology-04-00237.pdf (673 Kb)
PDF

Copyright Statement
Copyright information regarding this work can be found at the following address: http://creativecommons.org/licenses/by/4.0





You might also like



Downloadable Citations

;