STEPHEN HARDING STEVE.HARDING@NOTTINGHAM.AC.UK
Professor of Applied Biochemistry
Recent advances in the analysis of macromolecular interactions using the matrix-free method of sedimentation in the analytical ultracentrifuge
Harding, Stephen; Gillis, Richard; Almutairi, Fahad; Erten, Tayyibe; K�k, M.; Adams, Gary
Authors
Richard Gillis
Fahad Almutairi
Tayyibe Erten
M. K�k
Dr GARY ADAMS gary.adams@nottingham.ac.uk
Associate Professor
Abstract
Sedimentation in the analytical ultracentrifuge is a matrix free solution technique with no immobilisation, columns, or membranes required and can be used to study self-association and complex or “hetero”-interactions, stoichiometry, reversibility and interaction strength of a wide variety of macromolecular types and across a very large dynamic range (dissociation constants from 10−12 M to 10−1 M). We extend an earlier review specifically highlighting advances in sedimentation velocity and sedimentation equilibrium in the analytical ultracentrifuge applied to protein interactions and mucoadhesion and to review recent applications in protein self-association (tetanus toxoid, agrin), protein-like carbohydrate association (aminocelluloses), carbohydrate-protein interactions (polysaccharide-gliadin), nucleic-acid protein (G-duplexes), nucleic acid-carbohydrate (DNA-chitosan) and finally carbohydrate-carbohydrate (xanthan-chitosan and a ternary polysaccharide complex) interactions.
Citation
Harding, S., Gillis, R., Almutairi, F., Erten, T., Kök, M., & Adams, G. (2015). Recent advances in the analysis of macromolecular interactions using the matrix-free method of sedimentation in the analytical ultracentrifuge. Biology, 4(1), https://doi.org/10.3390/biology4010237
Journal Article Type | Article |
---|---|
Acceptance Date | Feb 16, 2015 |
Publication Date | Mar 6, 2015 |
Deposit Date | Apr 3, 2017 |
Publicly Available Date | Mar 29, 2024 |
Journal | Biology |
Electronic ISSN | 2079-7737 |
Publisher | MDPI |
Peer Reviewed | Peer Reviewed |
Volume | 4 |
Issue | 1 |
DOI | https://doi.org/10.3390/biology4010237 |
Keywords | protein; carbohydrate; nucleic acid; interaction; hydrodynamics |
Public URL | https://nottingham-repository.worktribe.com/output/748009 |
Publisher URL | http://www.mdpi.com/2079-7737/4/1/237 |
Files
biology-04-00237.pdf
(673 Kb)
PDF
Copyright Statement
Copyright information regarding this work can be found at the following address: http://creativecommons.org/licenses/by/4.0
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