Molecular weight analysis of starches: which technique?

Harding, Stephen E.; Adams, Gary G.; Gillis, Richard B.

Abstract

A number of methods are available to researchers for estimating molecular weights (molar masses) and molecular weight distributions of starches − or those in solution, using an appropriate solvent/solubilisation protocol. We outline the methods available and assess their relative merits and limitations. We focus on size-exclusion chromatography or field flow fractionation coupled to multi-angle light scattering, viscometry, and sedimentation velocity and sedimentation equilibrium in the analytical ultracentrifuge.

Citation

Harding, S. E., Adams, G. G., & Gillis, R. B. (2016). Molecular weight analysis of starches: which technique?. Starch/Stärke, 68(9-10), https://doi.org/10.1002/star.201600042

• Journal Article Type: Article
• Acceptance Date: Mar 5, 2016
• Online Publication Date: May 6, 2016
• Publication Date: Sep 7, 2016
• Deposit Date: Apr 3, 2017
• Journal: Starch - Stärke
• Print ISSN: 0038-9056
• Electronic ISSN: 1521-379X
• Publisher: Wiley-VCH Verlag
• Peer Reviewed: Peer Reviewed
• Volume: 68
• Issue: 9-10
• DOI: https://doi.org/10.1002/star.201600042
• Keywords: Hydrodynamics, Molecular weight, Solubility
• Public URL: https://nottingham-repository.worktribe.com/output/818723
• Publisher URL: https://doi.org/10.1002/star.201600042
• Related Public URLs: http://www.interscience.wiley.com/
• Contract Date: Apr 3, 2017