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Molecular weight analysis of starches: which technique?

Harding, Stephen E.; Adams, Gary G.; Gillis, Richard B.


Gary G. Adams

Richard B. Gillis


A number of methods are available to researchers for estimating molecular weights (molar masses) and molecular weight distributions of starches − or those in solution, using an appropriate solvent/solubilisation protocol. We outline the methods available and assess their relative merits and limitations. We focus on size-exclusion chromatography or field flow fractionation coupled to multi-angle light scattering, viscometry, and sedimentation velocity and sedimentation equilibrium in the analytical ultracentrifuge.


Harding, S. E., Adams, G. G., & Gillis, R. B. (2016). Molecular weight analysis of starches: which technique?. Starch/Stärke, 68(9-10), doi:10.1002/star.201600042

Journal Article Type Article
Acceptance Date Mar 5, 2016
Online Publication Date May 6, 2016
Publication Date Sep 7, 2016
Deposit Date Apr 3, 2017
Journal Starch - Stärke
Print ISSN 0038-9056
Electronic ISSN 1521-379X
Publisher Wiley-VCH Verlag
Peer Reviewed Peer Reviewed
Volume 68
Issue 9-10
Keywords Hydrodynamics, Molecular weight, Solubility
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