Fahad M. Almutairi
Hydrodynamic characterisation of chitosan and its interaction with two polyanions: DNA and xanthan
Almutairi, Fahad M.; Erten, Tayyibe; Adams, Gary G.; Hayes, Maria; McLoughlin, Pádraig; Kök, M. Şamil; Mackie, Alan R.; Rowe, Arthur J.; Harding, Stephen E.
Gary G. Adams
M. Şamil Kök
Alan R. Mackie
Arthur J. Rowe
STEPHEN HARDING firstname.lastname@example.org
Professor of Applied Biochemistry
Chitosan, a soluble polycationic derivative of insoluble chitin, has been widely considered for use in the food, cosmetic and pharmaceutical industries. Commercial (“C”) and in-house laboratory (“L”) prepared chitosan samples extracted from crustaceous shells with different molecular weight and degrees of acetylation (25% and 15%) were compared with regards to (i) weight–average molecular weight (Mw); (ii) sedimentation coefficient (so20,w) distribution, and (iii) intrinsic viscosity ([η]). These parameters were estimated using a combination of analytical ultracentrifugation (AUC), size exclusion chromatography coupled to multi-angle laser light scattering (SEC–MALS) and differential pressure viscometry. Polydisperse distributions were seen from sedimentation coefficient distributions and elution profiles from SEC–MALS. Mw values obtained for each sample by sedimentation equilibrium measurements were in excellent agreement with those obtained from SEC–MALS. Mark–Houwink–Kuhn–Sakurada (MHKS) and Wales van Holde analyses of the data all suggest a semi-flexible conformation.
The principle of co-sedimentation was then used to monitor the interactions of the two different molecular weights of L chitosans with two polyanions, DNA and xanthan (another double helical high molecular weight molecule). Interactions were clearly observed and then quantified from the changes in the sedimentation coefficient distribution of the mixture compared to unmixed controls using sedimentation velocity. The interactions appeared to show a strong dependence on molecular weight. The relevance of this for DNA condensation applications is indicated.
Almutairi, F. M., Erten, T., Adams, G. G., Hayes, M., McLoughlin, P., Kök, M. Ş., …Harding, S. E. (2015). Hydrodynamic characterisation of chitosan and its interaction with two polyanions: DNA and xanthan. Carbohydrate Polymers, 122, doi:10.1016/j.carbpol.2014.09.090
|Journal Article Type||Article|
|Acceptance Date||Sep 25, 2014|
|Online Publication Date||Oct 14, 2014|
|Publication Date||May 20, 2015|
|Deposit Date||May 11, 2017|
|Peer Reviewed||Peer Reviewed|
|Keywords||Sedimentation distribution; flexibility; co-sedimentation, double helix, multi-Gaussian fit|
|Copyright Statement||Copyright information regarding this work can be found at the following address: http://creativecommons.org/licenses/by-nc-nd/4.0|
This file is under embargo due to copyright reasons.
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