Research Repository

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DNA replication roadblocks caused by Cascade Interference complexes are alleviated by RecG DNA repair helicase (2018)
Journal Article
Killelea, T., Hawkins, M., Howard, J., McGlynn, P., & Bolt, E. L. (2018). DNA replication roadblocks caused by Cascade Interference complexes are alleviated by RecG DNA repair helicase. RNA Biology, doi:10.1080/15476286.2018.1496773. ISSN 1547-6286

Cascade complexes underpin E. coli CRISPR-Cas immunity systems by stimulating "adaptation" reactions that update immunity and by initiating "interference" reactions that destroy invader DNA. Recognition of invader DNA in Cascade catalysed R-loops pro... Read More

Identification of Escherichia coli ygaQ and rpmG as novel mitomycin C resistance factors implicated in DNA repair (2015)
Journal Article
Bolt, E. L., Jenkins, T., Russo, V. M., Ahmed, S., Cavey, J., & Cass, S. (2015). Identification of Escherichia coli ygaQ and rpmG as novel mitomycin C resistance factors implicated in DNA repair. Bioscience Reports, doi:10.1042/BSR20150249. ISSN 0144-8463

Using the ASKA (A Complete Set of E. coli K-12 ORF Archive) library for genome-wide screening of E. coli proteins we identified that expression of ygaQ and rpmG promotemitomycin C resistance (MMCR). YgaQ mediated MMCR was independent of homolog... Read More

Different genome stability proteins underpin primed and naïve adaptation in E. coli CRISPR-Cas immunity (2015)
Journal Article
and naïve adaptation in E. coli CRISPR-Cas immunity. Nucleic Acids Research, doi:10.1093/nar/gkv1213. ISSN 0305-1048

CRISPR-Cas is a prokaryotic immune system built from capture and integration of invader DNA into CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) loci, termed ‘Adaptation’, which is dependent on Cas1 and Cas2 proteins. In Escherichi... Read More

Intrinsic sequence specificity of the Cas1 integrase directs new spacer acquisition (2015)
Journal Article
Rollie, C., Schneider, S., Brinkmann, A. S., Bolt, E. L., & White, M. F. (2015). Intrinsic sequence specificity of the Cas1 integrase directs new spacer acquisition. eLife, doi:10.7554/eLife.08716. ISSN 2050-084X

The adaptive prokaryotic immune system CRISPR-Cas provides RNA-mediated protection from invading genetic elements. The fundamental basis of the system is the ability to capture small pieces of foreign DNA for incorporation into the genome at the CRIS... Read More