Identification of Escherichia coli ygaQ and rpmG as novel mitomycin C resistance factors implicated in DNA repair

Bolt, Edward L.; Jenkins, Tabitha; Russo, Valeria Moreira; Ahmed, Sharlene; Cavey, James; Cass, Simon

Abstract

Using the ASKA (A Complete Set of E. coli K-12 ORF Archive) library for genome-wide screening of E. coli proteins we identified that expression of ygaQ and rpmG promotemitomycin C resistance (MMCR). YgaQ mediated MMCR was independent of homologous recombination involving RecA or RuvABC, but required UvrD. YgaQ is an uncharacterized protein homologous to a-amylases that we identified to have nuclease activity directed to single stranded DNA of 5’ flaps. Nuclease activity was inactivated by mutation of two amino acid motifs, which also abolished MMCR. RpmG is frequently annotated as a bacterial ribosomal protein, although forms an operon with MutM glycosylase and a putative deubiquitinating enzyme, YicR. RpmG associated MMCR was dependent on MutM. MMCR from RpmG resembles DNA repair phenotypes reported for ‘idiosyncratic ribosomal proteins’ in eukaryotes.

Citation

Bolt, E. L., Jenkins, T., Russo, V. M., Ahmed, S., Cavey, J., & Cass, S. (2015). Identification of Escherichia coli ygaQ and rpmG as novel mitomycin C resistance factors implicated in DNA repair. Bioscience Reports, https://doi.org/10.1042/BSR20150249

• Journal Article Type: Article
• Publication Date: Dec 24, 2015
• Deposit Date: Jan 22, 2016
• Publicly Available Date: Jan 22, 2016
• Journal: Bioscience Reports
• Print ISSN: 0144-8463
• Electronic ISSN: 1573-4935
• Publisher: Portland Press
• Peer Reviewed: Peer Reviewed
• DOI: https://doi.org/10.1042/BSR20150249
• Public URL: https://nottingham-repository.worktribe.com/output/768683
• Publisher URL: http://www.bioscirep.org/content/early/2015/12/21/BSR20150249