All Outputs (105)

MicroRNA-511-3p Mediated Modulation of the Peroxisome Proliferator-Activated Receptor Gamma (PPARγ) Controls LPS-Induced Inflammatory Responses in Human Monocyte Derived DCs (2022)
Journal Article
Awuah, D., Ruisinger, A., Alobaid, M., Mbadugha, C., & Ghaemmaghami, A. M. (2022). MicroRNA-511-3p Mediated Modulation of the Peroxisome Proliferator-Activated Receptor Gamma (PPARγ) Controls LPS-Induced Inflammatory Responses in Human Monocyte Derived DCs. Immuno, 2(1), 104-117. https://doi.org/10.3390/immuno2010008

Abstract: The peroxisome proliferator-activated receptor gamma (PPAR) is a ligand-activated transcription factor expressed in dendritic cells (DCs), where it exerts anti-inflammatory responses against TLR4-induced inflammation. Recently, microRNA-511... Read More about MicroRNA-511-3p Mediated Modulation of the Peroxisome Proliferator-Activated Receptor Gamma (PPARγ) Controls LPS-Induced Inflammatory Responses in Human Monocyte Derived DCs.

Immune-instructive materials and surfaces for medical applications (2021)
Book Chapter
Fisher, L. E., & Ghaemmaghami, A. M. (2021). Immune-instructive materials and surfaces for medical applications. In S. F. Badylak, & J. H. Elisseeff (Eds.), Immunomodulatory Biomaterials: Regulating the Immune Response with Biomaterials to Affect Clinical Outcome (67-87). Elsevier. https://doi.org/10.1016/B978-0-12-821440-4.00008-6

Biomaterials are an integral part of modern medicine with diverse applications including drug delivery platforms, scaffolds for tissue engineering, biosensors and medical devices/implants. The aging population and steady increase in the prevalence of... Read More about Immune-instructive materials and surfaces for medical applications.

Immune-instructive materials as new tools for immunotherapy (2021)
Journal Article
Fisher, L. E., Kämmerling, L., Alexander, M. R., & Ghaemmaghami, A. M. (2022). Immune-instructive materials as new tools for immunotherapy. Current Opinion in Biotechnology, 74, 194-203. https://doi.org/10.1016/j.copbio.2021.11.005

Immune instructive materials, are materials with the ability to modulate or mimic the function of immune cells, provide exciting opportunities for developing new therapies in many areas including medical devices, chronic inflammation, cancer, and aut... Read More about Immune-instructive materials as new tools for immunotherapy.

Erratum: “Human lung-on-chips: Advanced systems for respiratory virus models and assessment of immune response” [Biomicrofluidics 15, 021501 (2021)] (2021)
Journal Article
Saygili, E., Yildiz-Ozturk, E., Green, M. J., Ghaemmaghami, A. M., & Yesil-Celiktas, O. (2021). Erratum: “Human lung-on-chips: Advanced systems for respiratory virus models and assessment of immune response” [Biomicrofluidics 15, 021501 (2021)]. Biomicrofluidics, 15(3), Article 039901. https://doi.org/10.1063/5.0056623

It has been drawn to the authors’ attention that our original article1 did not appropriately attribute portions of a figure that we had reused from Ref. 2. The figure caption as it should have appeared follows. FIG. 2. (a) Schematic depicting human l... Read More about Erratum: “Human lung-on-chips: Advanced systems for respiratory virus models and assessment of immune response” [Biomicrofluidics 15, 021501 (2021)].

Reversed-engineered human alveolar lung-on-a-chip model (2021)
Journal Article
Huang, D., Liu, T., Liao, J., Maharjan, S., Xie, X., Pérez, M., …Zhang, Y. S. (2021). Reversed-engineered human alveolar lung-on-a-chip model. Proceedings of the National Academy of Sciences, 118(19), Article e2016146118. https://doi.org/10.1073/pnas.2016146118

Here, we present a physiologically relevant model of the human pulmonary alveoli. This alveolar lung-on-a-chip platform is composed of a three-dimensional porous hydrogel made of gelatin methacryloyl with an inverse opal structure, bonded to a compar... Read More about Reversed-engineered human alveolar lung-on-a-chip model.

Mitigating the foreign body response through ‘immune-instructive’ biomaterials (2021)
Journal Article
Kämmerling, L., Fisher, L. E., Antmen, E., Simsek, G. M., Rostam, H. M., Vrana, N. E., & Ghaemmaghami, A. M. (2021). Mitigating the foreign body response through ‘immune-instructive’ biomaterials. Journal of Immunology and Regenerative Medicine, 12, Article 100040. https://doi.org/10.1016/j.regen.2021.100040

Objectives Biomaterials are routinely used in clinical applications. A key to the clinical success of implanted biomaterials is not eliciting detrimental immune responses. In this article, we provide an overview of immune responses to biomaterials,... Read More about Mitigating the foreign body response through ‘immune-instructive’ biomaterials.

Human lung-on-chips: Advanced systems for respiratory virus models and assessment of immune response (2021)
Journal Article
Saygili, E., Yildiz-Ozturk, E., Green, M. J., Ghaemmaghami, A. M., & Yesil-Celiktas, O. (2021). Human lung-on-chips: Advanced systems for respiratory virus models and assessment of immune response. Biomicrofluidics, 15(2), Article 021501. https://doi.org/10.1063/5.0038924

Respiratory viral infections are leading causes of death worldwide. A number of human respiratory viruses circulate in all age groups and adapt to person-to-person transmission. It is vital to understand how these viruses infect the host and how the... Read More about Human lung-on-chips: Advanced systems for respiratory virus models and assessment of immune response.

Development of a multiparametric (bio)sensing platform for continuous monitoring of stress metabolites (2021)
Journal Article
Chmayssem, A., Verplanck, N., Tanase, C. E., Costa, G., Monsalve-Grijalba, K., Amigues, S., …Mailley, P. (2021). Development of a multiparametric (bio)sensing platform for continuous monitoring of stress metabolites. Talanta, 229, Article 122275. https://doi.org/10.1016/j.talanta.2021.122275

There is a growing need for real-time monitoring of metabolic products that could reflect cell damages over extended periods. In this paper, we report the design and development of an original multiparametric (bio)sensing platform that is tailored fo... Read More about Development of a multiparametric (bio)sensing platform for continuous monitoring of stress metabolites.

Immunity in space: prokaryote adaptations and immune response in microgravity (2021)
Journal Article
Green, M. J., Aylott, J. W., Williams, P., Ghaemmaghami, A. M., & Williams, P. M. (2021). Immunity in space: prokaryote adaptations and immune response in microgravity. Life, 11(2), Article 112. https://doi.org/10.3390/life11020112

Immune dysfunction has long been reported by medical professionals regarding astronauts suffering from opportunistic infections both during their time in space and a short period afterwards once back on Earth. Various species of prokaryotes onboard t... Read More about Immunity in space: prokaryote adaptations and immune response in microgravity.

Corrigendum to ‘LC-MS metabolomics comparisons of cancer cell and macrophage responses to methotrexate and polymer-encapsulated methotrexate’ [International Journal of Pharmaceutics: X Volume 1 (2019) 100036] (2020)
Journal Article
Al-Natour, M. A., Alazzo, A., Ghaemmaghami, A. M., Kim, D. H., & Alexander, C. (2020). Corrigendum to ‘LC-MS metabolomics comparisons of cancer cell and macrophage responses to methotrexate and polymer-encapsulated methotrexate’ [International Journal of Pharmaceutics: X Volume 1 (2019) 100036]. International Journal of Pharmaceutics: X, 2, Article 100044. https://doi.org/10.1016/j.ijpx.2020.100044

© 2020 The Author(s) The authors regret that the original version of Fig. 3 incorrect. The updated figure is below for reference. [Figure presented] Fig. 3 Super-resolution microscopy image of methotrexate loaded PLGA nanoparticles (green) (92 μg/mL,... Read More about Corrigendum to ‘LC-MS metabolomics comparisons of cancer cell and macrophage responses to methotrexate and polymer-encapsulated methotrexate’ [International Journal of Pharmaceutics: X Volume 1 (2019) 100036].

Developing immune-regulatory materials using immobilized monosaccharides with immune-instructive properties (2020)
Journal Article
Alobaid, M. A., Richards, S., Alexander, M. R., Gibson, M. I., & Ghaemmaghami, A. M. (2020). Developing immune-regulatory materials using immobilized monosaccharides with immune-instructive properties. Materials Today Bio, 8, Article 100080. https://doi.org/10.1016/j.mtbio.2020.100080

© 2020 The Authors New strategies for immune modulation have shown real promise in regenerative medicine as well as the fight against autoimmune diseases, allergies, and cancer. Dendritic cells (DCs) are gatekeepers of the immune system and their abi... Read More about Developing immune-regulatory materials using immobilized monosaccharides with immune-instructive properties.

Facile Dye-Initiated Polymerization of Lactide–Glycolide Generates Highly Fluorescent Poly(lactic-co-glycolic Acid) for Enhanced Characterization of Cellular Delivery (2020)
Journal Article
Al-Natour, M. A., Yousif, M. D., Cavanagh, R., Abouselo, A., Apebende, E. A., Ghaemmaghami, A., …Alexander, C. (2020). Facile Dye-Initiated Polymerization of Lactide–Glycolide Generates Highly Fluorescent Poly(lactic-co-glycolic Acid) for Enhanced Characterization of Cellular Delivery. ACS Macro Letters, 9(3), 431-437. https://doi.org/10.1021/acsmacrolett.9b01014

Copyright © 2020 American Chemical Society. Poly(lactic-co-glycolic acid) (PLGA) is a versatile synthetic copolymer that is widely used in pharmaceutical applications. This is because it is well-tolerated in the body, and copolymers of varying physic... Read More about Facile Dye-Initiated Polymerization of Lactide–Glycolide Generates Highly Fluorescent Poly(lactic-co-glycolic Acid) for Enhanced Characterization of Cellular Delivery.

Metabolic characterisation of THP-1 macrophage polarisation using LC–MS-based metabolite profiling (2020)
Journal Article
Abuawad, A., Mbadugha, C., Ghaemmaghami, A. M., & Kim, D. (2020). Metabolic characterisation of THP-1 macrophage polarisation using LC–MS-based metabolite profiling. Metabolomics, 16(3), Article 33. https://doi.org/10.1007/s11306-020-01656-4

INTRODUCTION: Macrophages constitute a heterogeneous population of functionally distinct cells involved in several physiological and pathological processes. They display remarkable plasticity by changing their phenotype and function in response to en... Read More about Metabolic characterisation of THP-1 macrophage polarisation using LC–MS-based metabolite profiling.

LC-MS metabolomics comparisons of cancer cell and macrophage responses to methotrexate and polymer-encapsulated methotrexate (2019)
Journal Article
Al-Natour, M. A., Alazzo, A., Ghaemmaghami, A. M., Kim, D.-H., & Alexander, C. (2019). LC-MS metabolomics comparisons of cancer cell and macrophage responses to methotrexate and polymer-encapsulated methotrexate. International Journal of Pharmaceutics: X, 1, Article 100036. https://doi.org/10.1016/j.ijpx.2019.100036

Methotrexate (MTX) is a folate analogue antimetabolite widely used for the treatment of rheumatoid arthritis and cancer. A number of studies have shown that MTX delivered via nanoparticle carriers is more potent against cancer cells than free MTX, a... Read More about LC-MS metabolomics comparisons of cancer cell and macrophage responses to methotrexate and polymer-encapsulated methotrexate.

Editorial: Adverse reactions to biomaterials: State of the art in biomaterial risk assessment, immunomodulation and in vitro models for biomaterial testing (2019)
Journal Article
Vrana, N. E., Ghaemmaghami, A. M., & Zorlutuna, P. (2019). Editorial: Adverse reactions to biomaterials: State of the art in biomaterial risk assessment, immunomodulation and in vitro models for biomaterial testing. Frontiers in Bioengineering and Biotechnology, 7, Article 15. https://doi.org/10.3389/fbioe.2019.00015

Investigating the intracellular effects of hyperbranched polycation-DNA complexes on lung cancer cells using LC-MS-based metabolite profiling (2019)
Journal Article
Alazzo, A., Al-Natour, M., Spriggs, K., Stolnik, S., Ghaemmaghami, A., Kim, D.-H., & Alexander, C. (2019). Investigating the intracellular effects of hyperbranched polycation-DNA complexes on lung cancer cells using LC-MS-based metabolite profiling. Molecular Omics, 15(1), 77-87. https://doi.org/10.1039/C8MO00139A

Cationic polymers have emerged as a promising alternative to viral vectors in gene therapy. They are cheap to scale up, easy to functionalise and also presume to be safer than the viral vectors, however many of them are cytotoxic. The large number of... Read More about Investigating the intracellular effects of hyperbranched polycation-DNA complexes on lung cancer cells using LC-MS-based metabolite profiling.

A foreign body response-on-a-chip platform (2019)
Journal Article
Sharifi, F., Htwe, S. S., Righi, M., Liu, H., Peietralunga, A., Yesil-Celiktas, O., …Zhang, Y. S. (2019). A foreign body response-on-a-chip platform. Advanced Healthcare Materials, 8(4), 1-10. https://doi.org/10.1002/adhm.201801425

Understanding the foreign body response (FBR) and desiging strategies to modulate such a response represent a grand challenge for implant devices and biomaterials. Here, the development of a microfluidic platform is reported, i.e., the FBR‐on‐a‐chip... Read More about A foreign body response-on-a-chip platform.

New generation of bioreactors that advance extracellular matrix modelling and tissue engineering (2018)
Journal Article
Ahmed, S., Chauhan, V. M., Ghaemmaghami, A. M., & Aylott, J. W. (2019). New generation of bioreactors that advance extracellular matrix modelling and tissue engineering. Biotechnology Letters, 41(1), 1–25. https://doi.org/10.1007/s10529-018-2611-7

Bioreactors hold a lot of promise for tissue engineering and regenerative medicine applications. They have multiple uses including cell cultivation for therapeutic production and for in vitro organ modelling to provide a more physiologically relevant... Read More about New generation of bioreactors that advance extracellular matrix modelling and tissue engineering.

High-throughput approaches for screening and analysis of cell behaviors (2017)
Journal Article
Seo, J., Shin, J. Y., Leijten, J., Jeon, O., Camci-Unal, G., Dikina, A. D., …Khademhosseini, A. (2018). High-throughput approaches for screening and analysis of cell behaviors. Biomaterials, 153, 85-101. https://doi.org/10.1016/j.biomaterials.2017.06.022

The rapid development of new biomaterials and techniques to modify them challenge our capability to characterize them using conventional methods. In response, numerous high-throughput (HT) strategies are being developed to analyze biomaterials and th... Read More about High-throughput approaches for screening and analysis of cell behaviors.

Integrin-mediated interactions control macrophage polarization in 3D hydrogels (2017)
Journal Article
Cha, B.-H., Shin, S. R., Leijten, J., Li, Y.-C., Singh, S., Liu, J. C., …Khademhosseini, A. (2017). Integrin-mediated interactions control macrophage polarization in 3D hydrogels. Advanced Healthcare Materials, 6(21), Article 1700289. https://doi.org/10.1002/adhm.201700289

Adverse immune reactions prevent clinical translation of numerous implantable devices and materials. Although inflammation is an essential part of tissue regeneration, chronic inflammation ultimately leads to implant failure. In particular, macrophag... Read More about Integrin-mediated interactions control macrophage polarization in 3D hydrogels.