A foreign body response-on-a-chip platform

Sharifi, Fatemeh; Htwe, Su Su; Righi, Martina; Liu, Hua; Peietralunga, Anna; Yesil-Celiktas, Ozlem; Maharjan, Sushila; Cha, Byung-Hyun; Shin, Su-Ryon; Dokmeci, Mehmet Remzi; Vrana, Nihal Engin; Ghaemmaghammi, Amir M.; Khademhosseini, Ali; Zhang, Yu Shrike

Abstract

Understanding the foreign body response (FBR) and desiging strategies to modulate such a response represent a grand challenge for implant devices and biomaterials. Here, the development of a microfluidic platform is reported, i.e., the FBR‐on‐a‐chip (FBROC) for modeling the cascade of events during immune cell response to implants. The platform models the native implant microenvironment where the implants are interfaced directly with surrounding tissues, as well as vasculature with circulating immune cells. The study demonstrates that the release of cytokines such as monocyte chemoattractant protein 1 (MCP‐1) from the extracellular matrix (ECM)‐like hydrogels in the bottom tissue chamber induces trans‐endothelial migration of circulating monocytes in the vascular channel toward the hydrogels, thus mimicking implant‐induced inflammation. Data using patient‐derived peripheral blood mononuclear cells further reveal inter‐patient differences in FBR, highlighting the potential of this platform for monitoring FBR in a personalized manner. The prototype FBROC platform provides an enabling strategy to interrogate FBR on various implants, including biomaterials and engineered tissue constructs, in a physiologically relevant and individual‐specific manner.

Citation

Sharifi, F., Htwe, S. S., Righi, M., Liu, H., Peietralunga, A., Yesil-Celiktas, O., …Zhang, Y. S. (2019). A foreign body response-on-a-chip platform. Advanced Healthcare Materials, 8(4), 1-10. https://doi.org/10.1002/adhm.201801425

• Journal Article Type: Article
• Acceptance Date: Jan 2, 2019
• Online Publication Date: Jan 29, 2019
• Publication Date: Feb 21, 2019
• Deposit Date: Jan 23, 2019
• Publicly Available Date: Jan 30, 2020
• Journal: Advanced Healthcare Materials
• Print ISSN: 2192-2640
• Electronic ISSN: 2192-2659
• Publisher: Wiley
• Peer Reviewed: Peer Reviewed
• Volume: 8
• Issue: 4
• Article Number: 1801425
• Pages: 1-10
• DOI: https://doi.org/10.1002/adhm.201801425
• Keywords: foreign body response; immune response; implant, biomaterials; organ-on-a-chip
• Public URL: https://nottingham-repository.worktribe.com/output/1491360
• Publisher URL: https://onlinelibrary.wiley.com/doi/10.1002/adhm.201801425
• Additional Information: This is the peer reviewed version of the following article: F. Sharifi, S. S. Htwe, M. Righi, H. Liu, A. Pietralunga, O. Yesil‐Celiktas, S. Maharjan, B.‐H. Cha, S. R. Shin, M. R. Dokmeci, N. E. Vrana, A. M. Ghaemmaghami, A. Khademhosseini, Y. S. Zhang, Advanced Healthcare Materials 2019, 1801425. https://doi.org/10.1002/adhm.201801425. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions.
• Contract Date: Apr 2, 2019