High-throughput approaches for screening and analysis of cell behaviors

Seo, Jungmok; Shin, Jung Youn; Leijten, Jeroen; Jeon, Oju; Camci-Unal, Gulden; Dikina, Anna D.; Brinegar, Katelyn; Ghaemmaghami, Amir M.; Alsberg, Eben; Khademhosseini, Ali

Abstract

The rapid development of new biomaterials and techniques to modify them challenge our capability to characterize them using conventional methods. In response, numerous high-throughput (HT) strategies are being developed to analyze biomaterials and their interactions with cells using combinatorial approaches. Moreover, these systematic analyses have the power to uncover effects of delivered soluble bioactive molecules on cell responses. In this review, we describe the recent developments in HT approaches that help identify cellular microenvironments affecting cell behaviors and highlight HT screening of biochemical libraries for gene delivery, drug discovery, and toxicological studies. We also discuss HT techniques for the analyses of cell secreted biomolecules and provide perspectives on the future utility of HT approaches in biomedical engineering.

Citation

Seo, J., Shin, J. Y., Leijten, J., Jeon, O., Camci-Unal, G., Dikina, A. D., …Khademhosseini, A. (2018). High-throughput approaches for screening and analysis of cell behaviors. Biomaterials, 153, 85-101. https://doi.org/10.1016/j.biomaterials.2017.06.022

• Journal Article Type: Article
• Acceptance Date: Jun 19, 2017
• Online Publication Date: Nov 14, 2017
• Publication Date: Jan 1, 2018
• Deposit Date: Aug 19, 2022
• Journal: Biomaterials
• Print ISSN: 0142-9612
• Electronic ISSN: 1878-5905
• Publisher: Elsevier
• Peer Reviewed: Peer Reviewed
• Volume: 153
• Pages: 85-101
• DOI: https://doi.org/10.1016/j.biomaterials.2017.06.022
• Public URL: https://nottingham-repository.worktribe.com/output/3097094
• Publisher URL: https://www.sciencedirect.com/science/article/pii/S0142961217304209?via%3Dihub