Development of a multiparametric (bio)sensing platform for continuous monitoring of stress metabolites

Chmayssem, Ayman; Verplanck, Nicolas; Tanase, Constantin Edi; Costa, Guillaume; Monsalve-Grijalba, Karen; Amigues, Simon; Alias, M�lanie; Gougis, Maxime; Mourier, V�ronique; Vignoud, S�verine; Ghaemmaghami, Amir M.; Mailley, Pascal

Abstract

There is a growing need for real-time monitoring of metabolic products that could reflect cell damages over extended periods. In this paper, we report the design and development of an original multiparametric (bio)sensing platform that is tailored for the real-time monitoring of cell metabolites derived from cell cultures. Most attractive features of our developed electrochemical (bio)sensing platform are its easy manufacturing process, that enables seamless scale-up, modular and versatile approach, and low cost. In addition, the developed platform allows a multiparametric analysis instead of single-analyte analysis. Here we provide an overview of the sensors-based analysis of four main factors that can indicate a possible cell deterioration problem during cell-culture: pH, hydrogen peroxide, nitric oxide/nitrite and lactate. Herein, we are proposing a sensors platform based on thick-film coupled to microfluidic technology that can be integrated into any microfluidic system using Luer-lock connectors. This platform allows obtaining an accurate analysis of the secreting stress metabolites during cell/tissues culture.

Citation

Chmayssem, A., Verplanck, N., Tanase, C. E., Costa, G., Monsalve-Grijalba, K., Amigues, S., Alias, M., Gougis, M., Mourier, V., Vignoud, S., Ghaemmaghami, A. M., & Mailley, P. (2021). Development of a multiparametric (bio)sensing platform for continuous monitoring of stress metabolites. Talanta, 229, Article 122275. https://doi.org/10.1016/j.talanta.2021.122275

• Journal Article Type: Article
• Acceptance Date: Mar 1, 2021
• Online Publication Date: Mar 15, 2021
• Publication Date: Jul 1, 2021
• Deposit Date: Mar 18, 2021
• Publicly Available Date: Mar 18, 2021
• Journal: Talanta
• Print ISSN: 0039-9140
• Publisher: Elsevier
• Peer Reviewed: Peer Reviewed
• Volume: 229
• Article Number: 122275
• DOI: https://doi.org/10.1016/j.talanta.2021.122275
• Keywords: General Chemistry
• Public URL: https://nottingham-repository.worktribe.com/output/5400386
• Publisher URL: https://www.sciencedirect.com/science/article/abs/pii/S003991402100196X