All Outputs (27)

DirtyGenes: testing for significant changes in gene or bacterial population compositions from a small number of samples (2019)
Journal Article

High throughput genomics technologies are applied widely to microbiomes in humans, animals, soil and water, to detect changes in bacterial communities or the genes they carry, between different environments or treatments. We describe a method to test... Read More about DirtyGenes: testing for significant changes in gene or bacterial population compositions from a small number of samples.

Novel inflammatory cell infiltration scoring system to investigate healthy and footrot affected ovine interdigital skin (2018)
Journal Article

Ovine footrot is a degenerative disease of sheep feet leading to the separation of hoofhorn from the underlying skin and lameness. This study quantitatively examined histological features of the ovine interdigital skin as well as their relationship w... Read More about Novel inflammatory cell infiltration scoring system to investigate healthy and footrot affected ovine interdigital skin.

The applied development of a tiered multilocus sequence typing (MLST) scheme for Dichelobacter nodosus (2018)
Journal Article

Dichelobacter nodosus (D. nodosus) is the causative pathogen of ovine footrot, a disease that has a significant welfare and financial impact on the global sheep industry. Previous studies into the phylogenetics of D. nodosus have focused on Australia... Read More about The applied development of a tiered multilocus sequence typing (MLST) scheme for Dichelobacter nodosus.

A novel 3D skin explant model to study anaerobic bacterial infection (2017)
Journal Article

Skin infection studies are often limited by financial and ethical constraints, and alternatives, such as monolayer cell culture, do not reflect many cellular processes limiting their application. For a more functional replacement, 3D skin culture mod... Read More about A novel 3D skin explant model to study anaerobic bacterial infection.

A distinct bacterial dysbiosis associated skin inflammation in ovine footrot (2017)
Journal Article

Ovine footrot is a highly prevalent bacterial disease caused by Dichelobacter nodosus and characterised by the separation of the hoof horn from the underlying skin. The role of innate immune molecules and other bacterial communities in the developmen... Read More about A distinct bacterial dysbiosis associated skin inflammation in ovine footrot.

PIMMS (Pragmatic Insertional Mutation Mapping System) laboratory methodology a readily accessible tool for identification of essential genes in Streptococcus (2016)
Journal Article

The Pragmatic Insertional Mutation Mapping (PIMMS) laboratory protocol was developed alongside various bioinformatics packages (Blanchard et al., 2015) to enable detection of essential and conditionally essential genes in Streptococcus and related ba... Read More about PIMMS (Pragmatic Insertional Mutation Mapping System) laboratory methodology a readily accessible tool for identification of essential genes in Streptococcus.

Transposon insertion mapping with PIMMS, Pragmatic Insertional Mutation Mapping System (2015)
Journal Article

The PIMMS (Pragmatic Insertional Mutation Mapping System) pipeline has beendeveloped for simple conditionally essential genome discovery experiments in bacteria.Capable of using raw sequence data files alongside a FASTA sequence of thereference genom... Read More about Transposon insertion mapping with PIMMS, Pragmatic Insertional Mutation Mapping System.