Novel inflammatory cell infiltration scoring system to investigate healthy and footrot affected ovine interdigital skin

Agbaje, Michael; Rutland, Catrin S.; Maboni, Grazieli; Blanchard, Adam; Bexon, Melissa; Stewart, Ceri; Jones, Michael A.; Totemeyer, Sabine

Abstract

Ovine footrot is a degenerative disease of sheep feet leading to the separation of hoofhorn from the underlying skin and lameness. This study quantitatively examined histological features of the ovine interdigital skin as well as their relationship with pro-inflammatory cytokine (IL-1_) and virulent Dichelobacter nodosus in footrot. From 55 healthy and 30 footrot ovine feet, parallel biopsies (one fixed for histology) were collected post-slaughter and analysed for lesions and histopathological analysis using haematoxylin and eosin and Periodic Acid-Schiff. Histological lesions were similar in both conditions while inflammatory scores mirror IL-1_ expression levels. Increased inflammatory score corresponded with high virulent D. nodosus load and was significant (p

Citation

Agbaje, M., Rutland, C. S., Maboni, G., Blanchard, A., Bexon, M., Stewart, C., …Totemeyer, S. (2018). Novel inflammatory cell infiltration scoring system to investigate healthy and footrot affected ovine interdigital skin. PeerJ, 6, Article e5097. https://doi.org/10.7717/peerj.5097

• Journal Article Type: Article
• Acceptance Date: Jun 5, 2018
• Publication Date: Jul 2, 2018
• Deposit Date: Jul 2, 2018
• Publicly Available Date: Jul 2, 2018
• Journal: PeerJ
• Electronic ISSN: 2167-8359
• Publisher: PeerJ
• Peer Reviewed: Peer Reviewed
• Volume: 6
• Article Number: e5097
• DOI: https://doi.org/10.7717/peerj.5097
• Keywords: Footrot, Pro-inflammatory cytokine, Dichelobacter nodosus, Inflammation, Histopathology,; Ovine
• Public URL: https://nottingham-repository.worktribe.com/output/944666
• Publisher URL: https://peerj.com/articles/5097/