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High density SNP and DArT-based genetic linkage maps of two closely related oil palm populations

Gan, Siou Ting; Wong, Wei Chee; Wong, Choo Kien; Soh, Aik Chin; Kilian, Andrzej; Low, Eng-Ti Leslie; Massawe, Festo; Mayes, Sean

Authors

Siou Ting Gan

Wei Chee Wong

Choo Kien Wong

Aik Chin Soh soh.aikchin@cffresearch.org

Andrzej Kilian

Eng-Ti Leslie Low

Festo Massawe festo.massawe@nottingham.edu.my

Sean Mayes



Abstract

Oil palm (Elaeis guineensis Jacq.) is an outbreeding perennial tree crop with long breeding cycles, typically 12 years. Molecular marker technologies can greatly improve the breeding efficiency of oil palm. This study reports the first use of the DArTseq platform to genotype two closely related self-pollinated oil palm populations, namely AA0768 and AA0769 with 48 and 58 progeny respectively. Genetic maps were constructed using the DArT and SNP markers generated in combination with anchor SSR markers. Both maps consisted of 16 major independent linkage groups (2n = 2× = 32) with 1399 and 1466 mapped markers for the AA0768 and AA0769 populations, respectively, including the morphological trait “shell-thickness” (Sh). The map lengths were 1873.7 and 1720.6 cM with an average marker density of 1.34 and 1.17 cM, respectively. The integrated map was 1803.1 cM long with 2066 mapped markers and average marker density of 0.87 cM. A total of 82% of the DArTseq marker sequence tags identified a single site in the published genome sequence, suggesting preferential targeting of gene-rich regions by DArTseq markers. Map integration of higher density focused around the Sh region identified closely linked markers to the Sh, with D.15322 marker 0.24 cM away from the morphological trait and 5071 bp from the transcriptional start of the published SHELL gene. Identification of the Sh marker demonstrates the robustness of using the DArTseq platform to generate high density genetic maps of oil palm with good genome coverage. Both genetic maps and integrated maps will be useful for quantitative trait loci analysis of important yield traits as well as potentially assisting the anchoring of genetic maps to genomic sequences.

Journal Article Type Article
Journal Journal of Applied Genetics
Electronic ISSN 2190-3883
Publisher Humana Press
Peer Reviewed Peer Reviewed
APA6 Citation Gan, S. T., Wong, W. C., Wong, C. K., Soh, A. C., Kilian, A., Low, E. L., …Mayes, S. (in press). High density SNP and DArT-based genetic linkage maps of two closely related oil palm populations. Journal of Applied Genetics, doi:10.1007/s13353-017-0420-7
DOI https://doi.org/10.1007/s13353-017-0420-7
Keywords Elaeis, DArTseq, Genetic linkage map, Integrated map, Shell thickness
Publisher URL https://link.springer.com/article/10.1007%2Fs13353-017-0420-7
Copyright Statement Copyright information regarding this work can be found at the following address: http://eprints.nottingh.../end_user_agreement.pdf
Additional Information This is a post-peer-review, pre-copyedit version of an article published in Journal of Applied Genetics. The final authenticated version is available online at: http://dx.doi.org/10.1007/s13353-017-0420-7.

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