Development of ten microsatellite loci in the invasive giant African land snail, Achatina (=Lissachatina) fulica Bowdich, 1822

Morrison, C. L.; Springmann, M. J.; Iwanowicz, D. D.; Wade, C. M.

Abstract

© 2014, Springer Science+Business Media Dordrecht (outside the USA). A suite of tetra-nucleotide microsatellite loci were developed for the invasive giant African land snail, Achatina (=Lissachatina) fulica Bowdich, 1822, from Ion Torrent next-generation sequencing data. Ten of the 96 primer sets tested amplified consistently in 30 snails from Miami, Florida, plus 12 individuals representative of their native East Africa, Indian and Pacific Ocean regions. The loci displayed moderate levels of allelic diversity (average 5.6 alleles/locus) and heterozygosity (average 42 %). Levels of genetic diversity were sufficient to produce unique multi-locus genotypes and detect phylogeographic structuring among regional samples. The invasive A. fulica can cause extensive damage to important food crops and natural resources, including native flora and fauna. The loci characterized here will be useful for determining the origins and tracking the spread of invasions, detecting fine-scale spatial structuring and estimating demographic parameters.

• Journal Article Type: Article
• Acceptance Date: Sep 2, 2014
• Online Publication Date: Sep 19, 2014
• Publication Date: 2015-03
• Deposit Date: Aug 9, 2022
• Journal: Conservation Genetics Resources
• Print ISSN: 1877-7252
• Electronic ISSN: 1877-7260
• Publisher: Springer Verlag
• Peer Reviewed: Peer Reviewed
• Volume: 7
• Issue: 1
• Pages: 201-202
• DOI: https://doi.org/10.1007/s12686-014-0332-3
• Keywords: Genetics; Ecology, Evolution, Behavior and Systematics
• Public URL: https://nottingham-repository.worktribe.com/output/3189010
• Publisher URL: https://link.springer.com/article/10.1007/s12686-014-0332-3