Differentiation of Bulinus senegalensis and Bulinus forskalii Snails in West Africa Using Morphometric Analysis

Andrus, Peter S; Joof, Ebrima; Wade, Christopher M

Abstract

Purpose: Accurate identification of medically important intermediate host and vector species is crucial for understanding disease transmission and control. Identifying Bulinus snails which act as intermediate host species for the transmission of schistosomiasis is typically undertaken using conchological and genital morphology as well as molecular methods. Methods: Here, a landmark-based morphometric analysis of shell morphology was undertaken to determine its utility to distinguish the closely related and morphologically similar sister species Bulinus senegalensis and Bulinus forskalii. The method was developed to increase the accuracy of conchological morphology methods to identify Bulinus species in the field. Both species are found in West Africa, but only B. senegalensis is implicated in the transmission of urogenital schistosomiasis. Results: We found when scaled down to the same length, 3-whorl and 4-whorl (juvenile) B. senegalensis shells had a longer spire, narrower body whorl and shorter aperture than B. forskalii. In contrast, 5-whorl (adult) B. senegalensis had a shorter spire, but still had a shorter aperture and narrower body whorl than B. forskalii. Canonical Variate Analysis (CVA) showed minimal overlap between B. senegalensis and B. forskalii for 3-whorl and 4-whorl shells, with a clear separation for 5-whorl shells. Overall, B. senegalensis had a consistently shorter aperture size and narrower body whorl than B. forskalii for all development stages. Spire length was variable depending on the stage of development, with 3-whorl and 4-whorl shells having the opposite trends of adult shells. Conclusions: Our study demonstrates the applicability of landmark-based morphometrics in distinguishing the medically important, Bulinus senegalensis from its morphologically similar sister species, Bulinus forskalii. We recommend using measurements based on spire length, penultimate whorl length, body whorl width and aperture size to differentiate B. senegalensis and B. forskalii, when used with the appropriate information for each shell’s development stage.

Citation

Andrus, P. S., Joof, E., & Wade, C. M. (2024). Differentiation of Bulinus senegalensis and Bulinus forskalii Snails in West Africa Using Morphometric Analysis. Acta Parasitologica, 69, 1016–1026. https://doi.org/10.1007/s11686-024-00830-1

• Journal Article Type: Article
• Acceptance Date: Feb 26, 2024
• Online Publication Date: Mar 19, 2024
• Publication Date: Mar 19, 2024
• Deposit Date: Mar 26, 2024
• Publicly Available Date: Mar 27, 2024
• Journal: Acta Parasitologica
• Print ISSN: 1230-2821
• Electronic ISSN: 1896-1851
• Publisher: De Gruyter
• Peer Reviewed: Peer Reviewed
• Volume: 69
• Pages: 1016–1026
• DOI: https://doi.org/10.1007/s11686-024-00830-1
• Keywords: Morphometrics, Shell morphology, Schistosomiasis, Bulinus snails
• Public URL: https://nottingham-repository.worktribe.com/output/33020695
• Publisher URL: https://link.springer.com/article/10.1007/s11686-024-00830-1
• Additional Information: Received: 24 August 2022; Accepted: 26 February 2024; First Online: 19 March 2024; : ; : Not applicable.; : The authors declare no competing interests.