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Serial small- and wide-angle X-ray scattering with laboratory sources

Levenstein, Mark A.; Robertson, Karen; Turner, Thomas D.; Hunter, Liam; O'Brien, Cate; O'Shaughnessy, Cedrick; Kulak, Alexander N.; Le Magueres, Pierre; Wojciechowski, Jakub; Mykhaylyk, Oleksandr O.; Kapur, Nikil; Meldrum, Fiona C.

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Authors

Mark A. Levenstein

Thomas D. Turner

Liam Hunter

Cate O'Brien

Cedrick O'Shaughnessy

Alexander N. Kulak

Pierre Le Magueres

Jakub Wojciechowski

Oleksandr O. Mykhaylyk

Nikil Kapur

Fiona C. Meldrum



Abstract

Recent advances in X-ray instrumentation and sample injection systems have enabled serial crystallography of protein nanocrystals and the rapid structural analysis of dynamic processes. However, this progress has been restricted to large-scale X-ray free-electron laser (XFEL) and synchrotron facilities, which are often oversubscribed and have long waiting times. Here, we explore the potential of state-of-the-art laboratory X-ray systems to perform comparable analyses when coupled to micro- and millifluidic sample environments. Our results demonstrate that commercial small- and wide-angle X-ray scattering (SAXS/WAXS) instruments and X-ray diffractometers are ready to access samples and timescales (⪆5 ms) relevant to many processes in materials science including the preparation of pharmaceuticals, nanoparticles and functional crystalline materials. Tests of different X-ray instruments highlighted the importance of the optical configuration and revealed that serial WAXS/XRD analysis of the investigated samples was only possible with the higher flux of a microfocus setup. We expect that these results will also stimulate similar developments for structural biology.

Citation

Levenstein, M. A., Robertson, K., Turner, T. D., Hunter, L., O'Brien, C., O'Shaughnessy, C., …Meldrum, F. C. (2022). Serial small- and wide-angle X-ray scattering with laboratory sources. IUCrJ, 9(5), 538-543. https://doi.org/10.1107/S2052252522007631

Journal Article Type Article
Acceptance Date Jul 7, 2022
Online Publication Date Aug 13, 2022
Publication Date Sep 1, 2022
Deposit Date Oct 26, 2022
Publicly Available Date Oct 26, 2022
Journal IUCrJ
Electronic ISSN 2052-2525
Publisher International Union of Crystallography (IUCr)
Peer Reviewed Peer Reviewed
Volume 9
Issue 5
Pages 538-543
DOI https://doi.org/10.1107/S2052252522007631
Keywords Condensed Matter Physics; General Materials Science; Biochemistry; General Chemistry
Public URL https://nottingham-repository.worktribe.com/output/10082859
Publisher URL https://journals.iucr.org/m/issues/2022/05/00/lq5047/index.html
Additional Information Publication: IUCrJ; Content type: research letters; Article metrics: Available; Peer reviewed: Yes; Review process: Single blind; Received: 14 April 2022; Accepted: 26 July 2022; Published online: 13 August 2022; Supplementary materials: This article has supporting information; Copyright: © 2022 Mark A. Levenstein et al.; Licence: Creative Commons Attribution (CC-BY)

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