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Outputs (21)

Label-free Brillouin endo-microscopy for the quantitative 3D imaging of sub-micrometre biology (2024)
Journal Article
La Cavera, S., Chauhan, V. M., Hardiman, W., Yao, M., Fuentes-Domínguez, R., Setchfield, K., …Clark, M. (2024). Label-free Brillouin endo-microscopy for the quantitative 3D imaging of sub-micrometre biology. Communications Biology, 7(1), Article 451. https://doi.org/10.1038/s42003-024-06126-4

This report presents an optical fibre-based endo-microscopic imaging tool that simultaneously measures the topographic profile and 3D viscoelastic properties of biological specimens through the phenomenon of time-resolved Brillouin scattering. This u... Read More about Label-free Brillouin endo-microscopy for the quantitative 3D imaging of sub-micrometre biology.

Glycerol- and diglycerol-based polyesters: Evaluation of backbone alterations upon nano-formulation performance (2024)
Journal Article
Axioti, E., Dixon, E. G., Reynolds-Green, M., Alexander, E. C., Brugnoli, B., Keddie, D. J., …Taresco, V. (2024). Glycerol- and diglycerol-based polyesters: Evaluation of backbone alterations upon nano-formulation performance. Colloids and Surfaces B: Biointerfaces, 236, Article 113828. https://doi.org/10.1016/j.colsurfb.2024.113828

Despite the success of polyethylene glycol-based (PEGylated) polyesters in the drug delivery and biomedical fields, concerns have arisen regarding PEG's immunogenicity and limited biodegradability. In addition, inherent limitations, including limited... Read More about Glycerol- and diglycerol-based polyesters: Evaluation of backbone alterations upon nano-formulation performance.

Self-assembled chitosan-sodium usnate drug delivery nanosystems: Synthesis, characterization, stability studies, in vitro cytotoxicity and in vivo biocompatibility against 143 B cells (2023)
Journal Article
Brugnoli, B., Mariano, A., Simonis, B., Bombelli, C., Sennato, S., Piozzi, A., …Francolini, I. (2023). Self-assembled chitosan-sodium usnate drug delivery nanosystems: Synthesis, characterization, stability studies, in vitro cytotoxicity and in vivo biocompatibility against 143 B cells. Carbohydrate Polymer Technologies and Applications, 6, Article 100373. https://doi.org/10.1016/j.carpta.2023.100373

Polymers are among the most studied materials as drug carriers, due to their tunable chemical structure and ability to self-assemble to give different types of nanostructures. In this study, chitosan (CS) nanoparticles (NPs) were investigated as carr... Read More about Self-assembled chitosan-sodium usnate drug delivery nanosystems: Synthesis, characterization, stability studies, in vitro cytotoxicity and in vivo biocompatibility against 143 B cells.

Poly (diglycerol adipate) variants as enhanced nanocarrier replacements in drug delivery applications (2023)
Journal Article
Jacob, P. L., Brugnoli, B., Del Giudice, A., Phan, H., Chauhan, V. M., Beckett, L., …Taresco, V. (2023). Poly (diglycerol adipate) variants as enhanced nanocarrier replacements in drug delivery applications. Journal of Colloid and Interface Science, 641, 1043-1057. https://doi.org/10.1016/j.jcis.2023.03.124

Sustainably derived poly(glycerol adipate) (PGA) has been deemed to deliver all the desirable features expected in a polymeric scaffold for drug-delivery, including biodegradability, biocompatibility, self-assembly into nanoparticles (NPs) and a func... Read More about Poly (diglycerol adipate) variants as enhanced nanocarrier replacements in drug delivery applications.

Correction to “Facile Dye-Initiated Polymerization of Lactide−Glycolide Generates Highly Fluorescent Poly(lactic-coglycolic Acid) for Enhanced Characterization of Cellular Delivery” (2022)
Journal Article
Yousif, M. D., Al-Natour, M. A., Cavanagh, R., Abouselo, A., Apebende, E. A., Ghaemmaghami, A., …Alexander, C. (2022). Correction to “Facile Dye-Initiated Polymerization of Lactide−Glycolide Generates Highly Fluorescent Poly(lactic-coglycolic Acid) for Enhanced Characterization of Cellular Delivery”. ACS Macro Letters, 11, 1224. https://doi.org/10.1021/acsmacrolett.2c00570

There is a change to the order of authorship for this published Letter. The author list should appear as it does in this Addition and Correction. All authors have agreed to this change.

Gold–Oligonucleotide Nanoconstructs Engineered to Detect Conserved Enteroviral Nucleic Acid Sequences (2021)
Journal Article
Chauhan, V. M., Elsutohy, M. M., McClure, C. P., Irving, W. L., Roddis, N., & Aylott, J. W. (2021). Gold–Oligonucleotide Nanoconstructs Engineered to Detect Conserved Enteroviral Nucleic Acid Sequences. Biosensors, 11(7), Article 238. https://doi.org/10.3390/bios11070238

Enteroviruses are ubiquitous mammalian pathogens that can produce mild to life-threatening disease. We developed a multimodal, rapid, accurate and economical point-of-care biosensor that can detect nucleic acid sequences conserved amongst 96% of all... Read More about Gold–Oligonucleotide Nanoconstructs Engineered to Detect Conserved Enteroviral Nucleic Acid Sequences.

Fluorescent Nanosensors: Real-Time Biochemical Measurement for Cell and Gene Therapies (2020)
Journal Article
CHAUHAN, V. (2020). Fluorescent Nanosensors: Real-Time Biochemical Measurement for Cell and Gene Therapies. BioProcess International, 18(3), 4-8

Biochemical changes essential for cell growth occur within sub-cellular spaces, for which there are no readily available technologies to map their important ephemeral fluctuations. Fluorescent nanosensors, have the potential to address this challenge... Read More about Fluorescent Nanosensors: Real-Time Biochemical Measurement for Cell and Gene Therapies.

Facile Dye-Initiated Polymerization of Lactide–Glycolide Generates Highly Fluorescent Poly(lactic-co-glycolic Acid) for Enhanced Characterization of Cellular Delivery (2020)
Journal Article
Al-Natour, M. A., Yousif, M. D., Cavanagh, R., Abouselo, A., Apebende, E. A., Ghaemmaghami, A., …Alexander, C. (2020). Facile Dye-Initiated Polymerization of Lactide–Glycolide Generates Highly Fluorescent Poly(lactic-co-glycolic Acid) for Enhanced Characterization of Cellular Delivery. ACS Macro Letters, 9(3), 431-437. https://doi.org/10.1021/acsmacrolett.9b01014

Copyright © 2020 American Chemical Society. Poly(lactic-co-glycolic acid) (PLGA) is a versatile synthetic copolymer that is widely used in pharmaceutical applications. This is because it is well-tolerated in the body, and copolymers of varying physic... Read More about Facile Dye-Initiated Polymerization of Lactide–Glycolide Generates Highly Fluorescent Poly(lactic-co-glycolic Acid) for Enhanced Characterization of Cellular Delivery.

Intracellular processing of silica-coated superparamagnetic iron nanoparticles in human mesenchymal stem cells (2019)
Journal Article
Harrison, R. P., Chauhan, V. M., Onion, D., Aylott, J. W., & Sottile, V. (2019). Intracellular processing of silica-coated superparamagnetic iron nanoparticles in human mesenchymal stem cells. RSC Advances, 9(6), 3176-3184. https://doi.org/10.1039/C8RA09089K

Silica-coated Superparamagnetic Iron Nanoparticles (SiMAGs) are an exciting biomedical technology capable of targeted delivery of cell-based therapeutics and disease diagnosis. However, in order to realise their full clinical potential, their intrace... Read More about Intracellular processing of silica-coated superparamagnetic iron nanoparticles in human mesenchymal stem cells.

Augmenting automated analytics using fluorescent nanosensors (2018)
Journal Article
Chauhan, V. M. (2018). Augmenting automated analytics using fluorescent nanosensors. Cell & Gene Therapy Insights, 4(9), 837-850. https://doi.org/10.18609/cgti.2018.085

Cell and gene therapies (CGTs) are projected to transform healthcare precision in the biotherapeutics sector. However, for their true potential to be realised, advancements must be made to optimising their manufacture , such that CGT production is pr... Read More about Augmenting automated analytics using fluorescent nanosensors.

New generation of bioreactors that advance extracellular matrix modelling and tissue engineering (2018)
Journal Article
Ahmed, S., Chauhan, V. M., Ghaemmaghami, A. M., & Aylott, J. W. (2019). New generation of bioreactors that advance extracellular matrix modelling and tissue engineering. Biotechnology Letters, 41(1), 1–25. https://doi.org/10.1007/s10529-018-2611-7

Bioreactors hold a lot of promise for tissue engineering and regenerative medicine applications. They have multiple uses including cell cultivation for therapeutic production and for in vitro organ modelling to provide a more physiologically relevant... Read More about New generation of bioreactors that advance extracellular matrix modelling and tissue engineering.

Haematophagic Caenorhabditis elegans (2018)
Journal Article
Chauhan, V. M., & Pritchard, D. I. (2019). Haematophagic Caenorhabditis elegans. Parasitology, 146(3), 314-320. https://doi.org/10.1017/s0031182018001518

Caenorhabditis elegans is a free-living nematode that resides in soil and typically feeds on bacteria. We postulate that haematophagic C. elegans could provide a model to evaluate vaccine responses to intestinal proteins from hematophagous nematode p... Read More about Haematophagic Caenorhabditis elegans.

Enhanced distance-dependent fluorescence quenching using size tuneable core shell silica nanoparticles (2018)
Journal Article
Elsutohy, M. M., Selo, A., Chauhan, V. M., Tendler, S. J., & Aylott, J. W. (2018). Enhanced distance-dependent fluorescence quenching using size tuneable core shell silica nanoparticles. RSC Advances, 8(62), 35840-35848. https://doi.org/10.1039/C8RA05929B

Silica nanoparticles (SNPs) have been used as favoured platforms for sensor, drug delivery and biological imaging applications, due to their ease of synthesis, size-control and bespoke physico-chemical properties. In this study, we have developed a p... Read More about Enhanced distance-dependent fluorescence quenching using size tuneable core shell silica nanoparticles.

Enhancing cell and gene therapy manufacture through the application of advanced fluorescent optical sensors (Review) (2017)
Journal Article
Harrison, R. P., & Chauhan, V. M. (2018). Enhancing cell and gene therapy manufacture through the application of advanced fluorescent optical sensors (Review). Biointerphases, 13(1), Article 01A301-1-01A301-8. https://doi.org/10.1116/1.5013335

Cell and gene therapies (CGTs) are examples of future therapeutics that can be used to cure or alleviate the symptoms of disease, by repairing damaged tissue or reprogramming defective genetic information. However, despite the recent advancements in... Read More about Enhancing cell and gene therapy manufacture through the application of advanced fluorescent optical sensors (Review).

The physicochemical fingerprint of Necator americanus (2017)
Journal Article
Chauhan, V. M., Scurr, D. J., Christie, T., Telford, G., Aylott, J. W., & Pritchard, D. (in press). The physicochemical fingerprint of Necator americanus. PLoS Neglected Tropical Diseases, 11(12), Article e0005971. https://doi.org/10.1371/journal.pntd.0005971

Necator americanus, a haematophagous hookworm parasite, infects ~10% of the world’s population and is considered to be a significant public health risk. Its lifecycle has distinct stages, permitting its successful transit from the skin via the lungs... Read More about The physicochemical fingerprint of Necator americanus.

Real-time measurement of the intracellular pH of yeast cells during glucose metabolism using ratiometric fluorescent nanosensors (2017)
Journal Article
Elsutohy, M. M., Chauhan, V. M., Markus, R., Kyyaly, M. A., Tendler, S. J., & Aylott, J. W. (2017). Real-time measurement of the intracellular pH of yeast cells during glucose metabolism using ratiometric fluorescent nanosensors. Nanoscale, 9(18), 5904-5911. https://doi.org/10.1039/c7nr00906b

Intracellular pH is a key parameter that influences many biochemical and metabolic pathways that can also be used as an indirect marker to monitor metabolic and intracellular processes. Herein, we utilise ratiometric fluorescent pH-sensitive nanosens... Read More about Real-time measurement of the intracellular pH of yeast cells during glucose metabolism using ratiometric fluorescent nanosensors.

Quadruple labelled dual oxygen and pH-sensitive ratiometric nanosensors (2016)
Journal Article
Chauhan, V. M., Giuntini, F., & Aylott, J. W. (2016). Quadruple labelled dual oxygen and pH-sensitive ratiometric nanosensors. Sensing and Bio-Sensing Research, 8, https://doi.org/10.1016/j.sbsr.2016.03.007

Nanosensors capable of simultaneously measuring dissolved oxygen concentrations from 0 to 100% saturation and pH over the full physiological range, from pH 3.5 to 7.5, that advance the methods towards understanding of key biological gradients, were s... Read More about Quadruple labelled dual oxygen and pH-sensitive ratiometric nanosensors.

Controlled intracellular generation of reactive oxygen species in human mesenchymal stem cells using porphyrin conjugated nanoparticles (2015)
Journal Article
Lavado, A. S., Chauhan, V. M., Alhaj Zen, A., Giuntini, F., Jones, D. R. E., Boyle, R. W., …Aylott, J. W. (2015). Controlled intracellular generation of reactive oxygen species in human mesenchymal stem cells using porphyrin conjugated nanoparticles. Nanoscale, 7(34), https://doi.org/10.1039/c5nr00795j

Nanoparticles capable of generating controlled amounts of intracellular reactive oxygen species (ROS), that advance the study of oxidative stress and cellular communication, were synthesized by functionalizing polyacrylamide nanoparticles with zinc(I... Read More about Controlled intracellular generation of reactive oxygen species in human mesenchymal stem cells using porphyrin conjugated nanoparticles.