Biochemical changes essential for cell growth occur within sub-cellular spaces, for which there are no readily available technologies to map their important ephemeral fluctuations. Fluorescent nanosensors, have the potential to address this challenge, due to their biocompatibility, ratiometric accuracy and real-time measurement capabilities. They have already demonstrated their potential to quantify pH, molecular oxygen and temperature in complex model systems, which include nematodes, fungi, stem cells and electronic devices. Therefore, these exciting findings could be readily translated to augment biomanufacturing for cell and gene therapy future pharmaceuticals.
CHAUHAN, V. (2020). Fluorescent Nanosensors: Real-Time Biochemical Measurement for Cell and Gene Therapies. BioProcess International, 18(3), 4-8