Mutations in the binding site of TNFR1 PLAD reduce homologous interactions but can enhance antagonism of wild-type TNFR1 activity (2021)
Journal Article
Albogami, S., Todd, I., Negm, O., Fairclough, L. C., & Tighe, P. J. (2021). Mutations in the binding site of TNFR1 PLAD reduce homologous interactions but can enhance antagonism of wild-type TNFR1 activity. Immunology, https://doi.org/10.1111/imm.13400

The tumour necrosis factor receptor superfamily (TNFRSF) members contain cysteine-rich domains (CRD) in their extracellular regions, and the membrane-distal CRD1 forms homologous interactions in the absence of ligand. The CRD1 is therefore termed a p... Read More about Mutations in the binding site of TNFR1 PLAD reduce homologous interactions but can enhance antagonism of wild-type TNFR1 activity.

A Hybrid Evolutionary Strategy to Optimise Early-Stage Cancer Screening (2019)
Conference Proceeding
Figueredo, G. P., Shi, P., Parkes, A. J., Evans, K., Garibaldi, J. M., Negm, O., …Robertson, J. (2019). A Hybrid Evolutionary Strategy to Optimise Early-Stage Cancer Screening. In 2019 IEEE Congress on Evolutionary Computation (CEC) (95-102). https://doi.org/10.1109/CEC.2019.8790316

Current methods to identify cutoff values for tumour-associated molecules (antigens) discrimination are based on statistics and brute force. These methods applied to cancer screening problems are very inefficient, especially with large data sets with... Read More about A Hybrid Evolutionary Strategy to Optimise Early-Stage Cancer Screening.

Patients with TNF Receptor Associated Periodic Syndrome (TRAPS) are hypersensitive to Toll?like receptor 9 stimulation (2019)
Journal Article
Negm, O. H., Singh, S., Abduljabbar, W., Hamed, M. R., Radford, P., McDermott, E. M., …Tighe, P. J. (2019). Patients with TNF Receptor Associated Periodic Syndrome (TRAPS) are hypersensitive to Toll‐like receptor 9 stimulation. Clinical and Experimental Immunology, 197(3), 352-360. https://doi.org/10.1111/cei.13306

Tumour necrosis factor receptor‐associated periodic syndrome (TRAPS) is an hereditary autoinflammatory disorder characterised by recurrent episodes of fever and inflammation. It is associated with autosomal dominant mutations in TNFRSF1A, which encod... Read More about Patients with TNF Receptor Associated Periodic Syndrome (TRAPS) are hypersensitive to Toll?like receptor 9 stimulation.

Contribution of the alkylquinolone quorum sensing system to the interaction of Pseudomonas aeruginosa with bronchial epithelial cells. (2018)
Journal Article
Liu, Y., Hussain, F., Negm, O., Pavia, A., Halliday, N., Frédéric Dubern, J., …Martínez-Pomares, L. (2018). Contribution of the alkylquinolone quorum sensing system to the interaction of Pseudomonas aeruginosa with bronchial epithelial cells. Frontiers in Microbiology, 9, Article 3018. https://doi.org/10.3389/fmicb.2018.03018

Pseudomonas aeruginosa causes infections in patients with compromised epithelial 32 barrier function. Multiple virulence factors produced by P. aeruginosa are controlled 33 by quorum sensing (QS) via 2-alkyl-4(1H)-quinolone (AQ) signal molecules. Her... Read More about Contribution of the alkylquinolone quorum sensing system to the interaction of Pseudomonas aeruginosa with bronchial epithelial cells..

Tobacco smoke and nicotine suppress expression of activating signaling molecules in human dendritic cells (2018)
Journal Article
Alkhattabi, N., Todd, I., Negm, O., Tighe, P. J., & Fairclough, L. C. (2018). Tobacco smoke and nicotine suppress expression of activating signaling molecules in human dendritic cells. Toxicology Letters, 299, 40-46. https://doi.org/10.1016/j.toxlet.2018.09.002

Cigarette smoke has significant toxic effects on the immune system, and increases the risk of developing autoimmune diseases; one immunosuppressive effect of cigarette smoke is that it inhibits the T cell-stimulating, immunogenic properties of myeloi... Read More about Tobacco smoke and nicotine suppress expression of activating signaling molecules in human dendritic cells.

A protein microarray assay for serological determination of antigen-specific antibody responses following Clostridium difficile infection (2018)
Journal Article
Negm, O. H., Hamed, M. R., & Monaghan, T. M. (in press). A protein microarray assay for serological determination of antigen-specific antibody responses following Clostridium difficile infection. Journal of Visualized Experiments, 136, Article e57399. https://doi.org/10.3791/57399

We provide a detailed overview of a novel high-throughput protein microarray assay for the determination of anti-C. difficile antibody levels in human sera and in separate preparations of polyclonal IVIg. The protocol describes the methodological ste... Read More about A protein microarray assay for serological determination of antigen-specific antibody responses following Clostridium difficile infection.

Characterization of behavioral, signaling and cytokine alterations in a rat neurodevelopmental model for schizophrenia, and their reversal by the 5-HT6 receptor antagonist SB-399885 (2018)
Journal Article
Shortall, S. E., Negm, O. H., Fowler, M., Fairclough, L. C., Tighe, P. J., Wigmore, P. M., & King, M. (2018). Characterization of behavioral, signaling and cytokine alterations in a rat neurodevelopmental model for schizophrenia, and their reversal by the 5-HT6 receptor antagonist SB-399885. Molecular Neurobiology, 55(9), https://doi.org/10.1007/s12035-018-0940-0

Post-weaning social isolation of rats produces neuroanatomical, neurochemical and behavioral alterations resembling some core features of schizophrenia. This study examined the ability of the 5-HT? receptor antagonist SB-399885 to reverse isolation-i... Read More about Characterization of behavioral, signaling and cytokine alterations in a rat neurodevelopmental model for schizophrenia, and their reversal by the 5-HT6 receptor antagonist SB-399885.

Autoantibodies of IgM and IgG classes show differences in recognition of multiple autoantigens in chronic obstructive pulmonary disease (2017)
Journal Article
Shindi, R., Al-Mehairi, A., Negm, O. H., Kalsheker, N., Gale, N., Shale, D. J., …Fairclough, L. C. (2017). Autoantibodies of IgM and IgG classes show differences in recognition of multiple autoantigens in chronic obstructive pulmonary disease. Clinical Immunology, 183, https://doi.org/10.1016/j.clim.2017.09.020

Autoimmunity occurs in chronic obstructive pulmonary disease (COPD). We describe an antigen microarray for detecting serum autoantibodies (AAbs) to determine how IgM, as well as IgG, AAbs distinguish patients with COPD from controls with a history of... Read More about Autoantibodies of IgM and IgG classes show differences in recognition of multiple autoantigens in chronic obstructive pulmonary disease.

A signalome screening approach in the autoinflammatory disease TNF receptor associated periodic syndrome (TRAPS) highlights the anti-inflammatory properties of drugs for repurposing (2017)
Journal Article
Figueredo, G., Todd, I., Negm, O. H., Reps, J., Radford, P., Figueredo, G. P., …Tighe, P. J. (2017). A signalome screening approach in the autoinflammatory disease TNF receptor associated periodic syndrome (TRAPS) highlights the anti-inflammatory properties of drugs for repurposing. Pharmacological Research, 125, 188-200. https://doi.org/10.1016/j.phrs.2017.08.012

© 2017 Elsevier Ltd TNF receptor associated periodic syndrome (TRAPS) is an autoinflammatory disease caused by mutations in TNF Receptor 1 (TNFR1). Current therapies for TRAPS are limited and do not target the pro-inflammatory signalling pathways tha... Read More about A signalome screening approach in the autoinflammatory disease TNF receptor associated periodic syndrome (TRAPS) highlights the anti-inflammatory properties of drugs for repurposing.

High prevalence of subclass-specific binding and neutralizing antibodies against Clostridium difficile toxins in adult cystic fibrosis sera: possible mode of immunoprotection against symptomatic C. difficile infection (2017)
Journal Article
Monaghan, T. M., Negm, O. H., MacKenzie, B., Hamed, M. R., Shone, C. C., Humphreys, D., …Wilcox, M. H. (2017). High prevalence of subclass-specific binding and neutralizing antibodies against Clostridium difficile toxins in adult cystic fibrosis sera: possible mode of immunoprotection against symptomatic C. difficile infection. Clinical and Experimental Gastroenterology, 10, https://doi.org/10.2147/CEG.S133939

Objectives: Despite multiple risk factors and a high rate of colonization for Clostridium difficile, the occurrence of C. difficile infection in patients with cystic fibrosis is rare. The aim of this study was to compare the prevalence of binding C.... Read More about High prevalence of subclass-specific binding and neutralizing antibodies against Clostridium difficile toxins in adult cystic fibrosis sera: possible mode of immunoprotection against symptomatic C. difficile infection.