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Autoantibodies of IgM and IgG classes show differences in recognition of multiple autoantigens in chronic obstructive pulmonary disease

Shindi, Reham; Al-Mehairi, Amna; Negm, Ola H.; Kalsheker, Noor; Gale, Nichola; Shale, Dennis J.; Harrison, Timothy W.; Bolton, Charlotte E.; John, Michelle; Todd, Ian; Tighe, Patrick J.; Fairclough, Lucy C.

Authors

Reham Shindi

Amna Al-Mehairi

OLA NEGM ola.negm@nottingham.ac.uk
Assistant Professor

Noor Kalsheker

Nichola Gale

Dennis J. Shale

TIM HARRISON tim.harrison@nottingham.ac.uk
Professor of Asthma and Respiratory Medicine

Michelle John

Ian Todd

PATRICK TIGHE paddy.tighe@nottingham.ac.uk
Professor of Molecular Immunology



Abstract

Autoimmunity occurs in chronic obstructive pulmonary disease (COPD). We describe an antigen microarray for detecting serum autoantibodies (AAbs) to determine how IgM, as well as IgG, AAbs distinguish patients with COPD from controls with a history of smoking without COPD. All COPD patients' sera contained elevated levels of AAbs to some of 30 autoantigens. There were significant differences in the autoantigenic specificities of IgM AAbs compared to IgG AAbs in the COPD sera: for example, AAbs to histone and scl-70 were mainly IgG, whereas AAbs to CENP-B and La/ssB were mainly IgM; by contrast, IgM and IgG AAbs to collagen-V were equally prevalent. Thus, a combination of IgM and IgG AAbs specific for multiple autoantigens are detected in all cases of COPD at a level at which all non-COPD controls are negative for AAbs. This highlights the importance of different classes of AAbs to a range of autoantigens in COPD.

Citation

Shindi, R., Al-Mehairi, A., Negm, O. H., Kalsheker, N., Gale, N., Shale, D. J., …Fairclough, L. C. (2017). Autoantibodies of IgM and IgG classes show differences in recognition of multiple autoantigens in chronic obstructive pulmonary disease. Clinical Immunology, 183, https://doi.org/10.1016/j.clim.2017.09.020

Journal Article Type Article
Acceptance Date Sep 22, 2017
Online Publication Date Sep 23, 2017
Publication Date Oct 1, 2017
Deposit Date Oct 3, 2017
Publicly Available Date Mar 29, 2024
Journal Clinical Immunology
Print ISSN 1521-6616
Electronic ISSN 1521-7035
Publisher Elsevier
Peer Reviewed Peer Reviewed
Volume 183
DOI https://doi.org/10.1016/j.clim.2017.09.020
Keywords Chronic obstructive pulmonary disease; Autoimmunity; Autoantibodies; Autoantigens; Protein microarray
Public URL https://nottingham-repository.worktribe.com/output/966264
Publisher URL http://www.sciencedirect.com/science/article/pii/S1521661617301808

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