The application of protein microarray assays in psychoneuroimmunology

Ayling, K.; Bowden, T.; Tighe, P.; Todd, I.; Dilnot, Elizabeth M.; Negm, O.H.; Fairclough, L.; Vedhara, K.

Abstract

Protein microarrays are miniaturized multiplex assays that exhibit many advantages over the commonly used enzyme-linked immunosorbent assay (ELISA). This article aims to introduce protein microarrays to readers of Brain, Behavior, and Immunity and demonstrate its utility and validity for use in psychoneuroimmunological research. As part of an ongoing investigation of psychological and behavioral influences on influenza vaccination responses, we optimized a novel protein microarray to quantify influenza-specific antibody levels in human sera. Reproducibility was assessed by calculating intra- and inter-assay coefficients of variance on serially diluted human IgG concentrations. A random selection of samples was analyzed by microarray and ELISA to establish validity of the assay. For IgG concentrations, intra-assay and inter-assay precision profiles demonstrated a mean coefficient of variance of 6.7% and 11.5% respectively. Significant correlations were observed between microarray and ELISA for all antigens, demonstrating the microarray is a valid alternative to ELISA. Protein microarrays are a highly robust, novel assay method that could be of significant benefit for researchers working in psychoneuroimmunology. They offer high throughput, fewer resources per analyte and can examine concurrent neuro-immune-endocrine mechanisms.

Citation

Ayling, K., Bowden, T., Tighe, P., Todd, I., Dilnot, E. M., Negm, O., …Vedhara, K. (2017). The application of protein microarray assays in psychoneuroimmunology. Brain, Behavior, and Immunity, 59, 62-66. https://doi.org/10.1016/j.bbi.2016.09.013

• Journal Article Type: Article
• Acceptance Date: Sep 13, 2016
• Online Publication Date: Sep 14, 2016
• Publication Date: 2017-01
• Deposit Date: Sep 23, 2016
• Publicly Available Date: Sep 23, 2016
• Journal: Brain, Behavior and Immunity
• Print ISSN: 0889-1591
• Electronic ISSN: 1090-2139
• Publisher: Elsevier
• Peer Reviewed: Peer Reviewed
• Volume: 59
• Pages: 62-66
• DOI: https://doi.org/10.1016/j.bbi.2016.09.013
• Keywords: Microarray; ELISA; Psychoneuroimmunology; Sera; Multiplex; Assay
• Public URL: https://nottingham-repository.worktribe.com/output/838044
• Publisher URL: http://www.sciencedirect.com/science/article/pii/S0889159116304226
• Additional Information: © 2016. This manuscript version is made available under the CC-BY-NC-ND 4.0 license https://creativecommons.org/licenses/by-nc-nd/4.0/
• Contract Date: Sep 23, 2016