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The application of protein microarray assays in psychoneuroimmunology

Ayling, K.; Bowden, T.; Tighe, P.; Todd, I.; Dilnot, Elizabeth M.; Negm, O.H.; Fairclough, L.; Vedhara, K.

Authors

T. Bowden

I. Todd

Elizabeth M. Dilnot liz.abbott@nottingham.ac.uk

OLA NEGM ola.negm@nottingham.ac.uk
Teaching Associate and Problembased Learning Facilitator

KAVITA VEDHARA kavita.vedhara@nottingham.ac.uk
Professor in Applied Psychology



Abstract

Protein microarrays are miniaturized multiplex assays that exhibit many advantages over the commonly used enzyme-linked immunosorbent assay (ELISA). This article aims to introduce protein microarrays to readers of Brain, Behavior, and Immunity and demonstrate its utility and validity for use in psychoneuroimmunological research. As part of an ongoing investigation of psychological and behavioral influences on influenza vaccination responses, we optimized a novel protein microarray to quantify influenza-specific antibody levels in human sera. Reproducibility was assessed by calculating intra- and inter-assay coefficients of variance on serially diluted human IgG concentrations. A random selection of samples was analyzed by microarray and ELISA to establish validity of the assay. For IgG concentrations, intra-assay and inter-assay precision profiles demonstrated a mean coefficient of variance of 6.7% and 11.5% respectively. Significant correlations were observed between microarray and ELISA for all antigens, demonstrating the microarray is a valid alternative to ELISA. Protein microarrays are a highly robust, novel assay method that could be of significant benefit for researchers working in psychoneuroimmunology. They offer high throughput, fewer resources per analyte and can examine concurrent neuro-immune-endocrine mechanisms.

Journal Article Type Article
Publication Date 2017-01
Journal Brain, Behavior and Immunity
Print ISSN 0889-1591
Electronic ISSN 1090-2139
Publisher Elsevier
Peer Reviewed Peer Reviewed
Volume 59
Pages 62-66
APA6 Citation Ayling, K., Bowden, T., Tighe, P., Todd, I., Dilnot, E. M., Negm, O., …Vedhara, K. (2017). The application of protein microarray assays in psychoneuroimmunology. Brain, Behavior, and Immunity, 59, 62-66. https://doi.org/10.1016/j.bbi.2016.09.013
DOI https://doi.org/10.1016/j.bbi.2016.09.013
Keywords Microarray; ELISA; Psychoneuroimmunology; Sera; Multiplex; Assay
Publisher URL http://www.sciencedirect.com/science/article/pii/S0889159116304226
Copyright Statement Copyright information regarding this work can be found at the following address: http://creativecommons.org/licenses/by-nc-nd/4.0
Additional Information © 2016. This manuscript version is made available under the CC-BY-NC-ND 4.0 license https://creativecommons.org/licenses/by-nc-nd/4.0/

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Copyright Statement
Copyright information regarding this work can be found at the following address: http://creativecommons.org/licenses/by-nc-nd/4.0





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