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The application of protein microarray assays in psychoneuroimmunology

Ayling, K.; Bowden, T.; Tighe, P.; Todd, I.; Dilnot, Elizabeth M.; Negm, O.H.; Fairclough, L.; Vedhara, K.

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T. Bowden

Professor of Molecular Immunology

I. Todd

Elizabeth M. Dilnot

Assistant Professor

K. Vedhara


Protein microarrays are miniaturized multiplex assays that exhibit many advantages over the commonly used enzyme-linked immunosorbent assay (ELISA). This article aims to introduce protein microarrays to readers of Brain, Behavior, and Immunity and demonstrate its utility and validity for use in psychoneuroimmunological research. As part of an ongoing investigation of psychological and behavioral influences on influenza vaccination responses, we optimized a novel protein microarray to quantify influenza-specific antibody levels in human sera. Reproducibility was assessed by calculating intra- and inter-assay coefficients of variance on serially diluted human IgG concentrations. A random selection of samples was analyzed by microarray and ELISA to establish validity of the assay. For IgG concentrations, intra-assay and inter-assay precision profiles demonstrated a mean coefficient of variance of 6.7% and 11.5% respectively. Significant correlations were observed between microarray and ELISA for all antigens, demonstrating the microarray is a valid alternative to ELISA. Protein microarrays are a highly robust, novel assay method that could be of significant benefit for researchers working in psychoneuroimmunology. They offer high throughput, fewer resources per analyte and can examine concurrent neuro-immune-endocrine mechanisms.

Journal Article Type Article
Acceptance Date Sep 13, 2016
Online Publication Date Sep 14, 2016
Publication Date 2017-01
Deposit Date Sep 23, 2016
Publicly Available Date Sep 23, 2016
Journal Brain, Behavior and Immunity
Print ISSN 0889-1591
Electronic ISSN 1090-2139
Publisher Elsevier
Peer Reviewed Peer Reviewed
Volume 59
Pages 62-66
Keywords Microarray; ELISA; Psychoneuroimmunology; Sera; Multiplex; Assay
Public URL
Publisher URL
Additional Information © 2016. This manuscript version is made available under the CC-BY-NC-ND 4.0 license


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