Outputs (29)

Synthetic macromolecular peptide-mimetics with amino acid substructure residues as protein stabilising excipients (2023)
Journal Article
Foralosso, R., Kopiasz, R. J., Mantovani, G., Stolnik, S., & Alexander, C. (2024). Synthetic macromolecular peptide-mimetics with amino acid substructure residues as protein stabilising excipients. Journal of Materials Chemistry B, 12, 1022-1030. https://doi.org/10.1039/d3tb02102e

The clinical use of protein and peptide biotherapeutics requires fabrication of stable products. This particularly concerns stability towards aggregation of proteins or peptides. Here, we tested a hypothesis that interactions between a synthetic pept... Read More about Synthetic macromolecular peptide-mimetics with amino acid substructure residues as protein stabilising excipients.

Sulfation at Glycopolymer Side Chains Switches Activity at the Macrophage Mannose Receptor (CD206) In Vitro and In Vivo (2022)
Journal Article
Mastrotto, F., Pirazzini, M., Negro, S., Salama, A., Martinez-Pomares, L., & Mantovani, G. (2022). Sulfation at Glycopolymer Side Chains Switches Activity at the Macrophage Mannose Receptor (CD206) In Vitro and In Vivo. Journal of the American Chemical Society, 144(50), 23134-23147. https://doi.org/10.1021/jacs.2c10757

The mannose receptor (CD206) is an endocytic receptor expressed by selected innate immune cells and nonvascular endothelium, which plays a critical role in both homeostasis and pathogen recognition. Although its involvement in the development of seve... Read More about Sulfation at Glycopolymer Side Chains Switches Activity at the Macrophage Mannose Receptor (CD206) In Vitro and In Vivo.

Sulfation at Glycopolymer Side Chains Switches Activity at the Macrophage Mannose Receptor (CD206) In Vitro and In Vivo (2022)
Journal Article
Mastrotto, F., Pirazzini, M., Negro, S., Salama, A., Martinez-Pomares, L., & Mantovani, G. (2022). Sulfation at Glycopolymer Side Chains Switches Activity at the Macrophage Mannose Receptor (CD206) In Vitro and In Vivo. Journal of the American Chemical Society, 144(50), 23134–23147. https://doi.org/10.1021/jacs.2c10757

The mannose receptor (CD206) is an endocytic receptor expressed by selected innate immune cells and nonvascular endothelium, which plays a critical role in both homeostasis and pathogen recognition. Although its involvement in the development of seve... Read More about Sulfation at Glycopolymer Side Chains Switches Activity at the Macrophage Mannose Receptor (CD206) In Vitro and In Vivo.

Direct routes to functional RAFT agents from substituted N-alkyl maleimides (2022)
Journal Article
Catania, R., Foralosso, R., Spanos, L., Russo, E., Mastrotto, F., Gurnani, P., …Mantovani, G. (2022). Direct routes to functional RAFT agents from substituted N-alkyl maleimides. Polymer Chemistry, 13(45), 6261-6267. https://doi.org/10.1039/d1py01565f

N-substituted maleimides have become an indispensable tool for the synthesis of bioconjugates and functional materials. Herein, we present three strategies for the incorporation of N-alkyl substituted maleimides into RAFT agents and show that these m... Read More about Direct routes to functional RAFT agents from substituted N-alkyl maleimides.

A mechanoresponsive nano-sized carrier achieves intracellular release of drug on external ultrasound stimulus (2022)
Journal Article
Catania, R., Onion, D., Russo, E., Zelzer, M., Mantovani, G., Huett, A., & Stolnik, S. (2022). A mechanoresponsive nano-sized carrier achieves intracellular release of drug on external ultrasound stimulus. RSC Advances, 12(26), 16561-16569. https://doi.org/10.1039/d2ra02307e

Control over intracellular release of therapeutic compounds incorporated into nano-carriers will open new possibilities for targeted treatments of various diseases including cancer, and viral and bacterial infections. Here we report our study on mech... Read More about A mechanoresponsive nano-sized carrier achieves intracellular release of drug on external ultrasound stimulus.

Combining Inducible Lectin Expression and Magnetic Glyconanoparticles for the Selective Isolation of Bacteria from Mixed Populations (2021)
Journal Article
Petch, J. E., Gurnani, P., Yilmaz, G., Mastrotto, F., Alexander, C., Heeb, S., …Mantovani, G. (2021). Combining Inducible Lectin Expression and Magnetic Glyconanoparticles for the Selective Isolation of Bacteria from Mixed Populations. ACS Applied Materials and Interfaces, 13(16), 19230-19243. https://doi.org/10.1021/acsami.1c00907

The selective isolation of bacteria from mixed populations has been investigated in varied applications ranging from differential pathogen identification in medical diagnostics and food safety to the monitoring of microbial stress dynamics in industr... Read More about Combining Inducible Lectin Expression and Magnetic Glyconanoparticles for the Selective Isolation of Bacteria from Mixed Populations.

A Reactive Prodrug Ink Formulation Strategy for Inkjet 3D Printing of Controlled Release Dosage Forms and Implants (2020)
Journal Article
He, Y., Foralosso, R., Ferraz Trindade, G., Ilchev, A., Cantu, L. R., Clark, E., …Wildman, R. D. (2020). A Reactive Prodrug Ink Formulation Strategy for Inkjet 3D Printing of Controlled Release Dosage Forms and Implants. Advanced Therapeutics, 3(6), Article 1900187. https://doi.org/10.1002/adtp.201900187

We propose a strategy for creating tuneable 3D printed drug delivery devices. 3D printing offers the opportunity for improved compliance and patient treatment outcomes through personalisation, but bottlenecks include finding formulations that provide... Read More about A Reactive Prodrug Ink Formulation Strategy for Inkjet 3D Printing of Controlled Release Dosage Forms and Implants.

Targeted PEG-poly(glutamic acid) complexes for inhalation protein delivery to the lung (2019)
Journal Article
Nieto-Orellana, A., Li, H., Rosiere, R., Wauthoz, N., Williams, H., Monteiro, C., …Stolnik, S. (2019). Targeted PEG-poly(glutamic acid) complexes for inhalation protein delivery to the lung. Journal of Controlled Release, 316, 250-262. https://doi.org/10.1016/j.jconrel.2019.10.012

Pulmonary delivery is increasingly seen as an attractive, non-invasive route for the delivery of forthcoming protein therapeutics. In this context, here we describe protein complexes with a new ‘complexing excipient’ - vitamin B12-targeted poly(ethyl... Read More about Targeted PEG-poly(glutamic acid) complexes for inhalation protein delivery to the lung.

Poly(triazolyl methacrylate) glycopolymers as potential targeted unimolecular nanocarriers (2019)
Journal Article
Madeira Do O, J., Foralosso, R., Yilmaz, G., Mastrotto, F., King, P. J. S., Xerri, R. M., …Mantovani, G. (2019). Poly(triazolyl methacrylate) glycopolymers as potential targeted unimolecular nanocarriers. Nanoscale, 11(44), 21155-21166. https://doi.org/10.1039/C9NR05836B

Synthetic glycopolymers are increasingly investigated as multivalent ligands for a range of biological and biomedical applications. This study indicates that glycopolymers with fine-tuned balance between hydrophilic sugar pendant units and relatively... Read More about Poly(triazolyl methacrylate) glycopolymers as potential targeted unimolecular nanocarriers.

Polyvalent diazonium polymers provide efficient protection of oncolytic adenovirus Enadenotucirev from neutralizing antibodies while maintaining biological activity in vitro and in vivo (2019)
Journal Article
Francini, N., Cochrane, D., Illingworth, S., Purdie, L., Mantovani, G., Fisher, K., …Alexander, C. (2019). Polyvalent diazonium polymers provide efficient protection of oncolytic adenovirus Enadenotucirev from neutralizing antibodies while maintaining biological activity in vitro and in vivo. Bioconjugate Chemistry, 30(4), 1244-1257. https://doi.org/10.1021/acs.bioconjchem.9b00189

Oncolytic viruses offer many advantages for cancer therapy when administered directly to confined solid tumors. However, the systemic delivery of these viruses is problematic because of the host immune response, undesired interactions with blood comp... Read More about Polyvalent diazonium polymers provide efficient protection of oncolytic adenovirus Enadenotucirev from neutralizing antibodies while maintaining biological activity in vitro and in vivo.

Synthesis of nucleoside-boronic esters hydrophobic pro-drugs: a possible route to improve hydrophilic nucleoside drug loading into polymer nanoparticles (2018)
Journal Article
Abo-zeid, Y., Mantovani, G., Irving, W. L., & Garnett, M. (2018). Synthesis of nucleoside-boronic esters hydrophobic pro-drugs: a possible route to improve hydrophilic nucleoside drug loading into polymer nanoparticles. Journal of Drug Delivery Science and Technology, 46, https://doi.org/10.1016/j.jddst.2018.05.027

Nucleoside analogues are active therapeutic agents for different types of diseases e.g. Cancer and virus infections. However, they are associated with several side effects due to off-target accumulation. Particulate delivery systems such as nanoparti... Read More about Synthesis of nucleoside-boronic esters hydrophobic pro-drugs: a possible route to improve hydrophilic nucleoside drug loading into polymer nanoparticles.

Water-soluble substituted chitosan derivatives as technology platform for inhalation delivery of siRNA (2018)
Journal Article
Capel, V., Vllasaliu, D., Watts, P., Clarke, P. A., Luxton, D., Grabowska, A. M., …Stolnik, S. (in press). Water-soluble substituted chitosan derivatives as technology platform for inhalation delivery of siRNA. Drug Delivery, 25(1), https://doi.org/10.1080/10717544.2018.1440668

Despite research efforts full potential of siRNA-based therapeutics has not yet been fully realized due to a need for suitable, effective delivery formulations. Here, we examine a potential of a new class of water-soluble chitosans as siRNA platform... Read More about Water-soluble substituted chitosan derivatives as technology platform for inhalation delivery of siRNA.

Switching of Macromolecular Ligand Display by Thermoresponsive Polymers Mediates Endocytosis of Multiconjugate Nanoparticles (2018)
Journal Article
Sayers, E., Magnusson, J. P., Moody, P., Mastrotto, F., Conte, C., Brazzale, C., …Alexander, C. (2018). Switching of Macromolecular Ligand Display by Thermoresponsive Polymers Mediates Endocytosis of Multiconjugate Nanoparticles. Bioconjugate Chemistry, 29(4), 1030-1046. https://doi.org/10.1021/acs.bioconjchem.7b00704

© 2018 American Chemical Society. Ligand-mediated targeting and internalization of plasma membrane receptors is central to cellular function. These types of receptors have accordingly been investigated as targets to facilitate entry of diagnostic and... Read More about Switching of Macromolecular Ligand Display by Thermoresponsive Polymers Mediates Endocytosis of Multiconjugate Nanoparticles.

Dry-powder formulations of non-covalent protein complexes with linear or miktoarm copolymers for pulmonary delivery (2018)
Journal Article
Nieto-Orellana, A., Coghlan, D., Rothery, M., Falcone, F. H., Bosquillon, C., Childerhouse, N., …Stolnik, S. (2018). Dry-powder formulations of non-covalent protein complexes with linear or miktoarm copolymers for pulmonary delivery. International Journal of Pharmaceutics, 540(1-2), https://doi.org/10.1016/j.ijpharm.2018.02.008

Pulmonary delivery of protein therapeutics has considerable clinical potential for treating both local and systemic diseases. However, poor protein conformational stability, immunogenicity and protein degradation by proteolytic enzymes in the lung ar... Read More about Dry-powder formulations of non-covalent protein complexes with linear or miktoarm copolymers for pulmonary delivery.

Synthetic glycopolymers as modulators of protein aggregation: influence of chemical composition, topology and concentration (2018)
Journal Article
Madeira do Ó, J., Mastrotto, F., Francini, N., Allen, S., van der Walle, C., Stolnik, S., & Mantovani, G. (in press). Synthetic glycopolymers as modulators of protein aggregation: influence of chemical composition, topology and concentration. Journal of Materials Chemistry B, 7(6), https://doi.org/10.1039/c7tb02720f

Novel drug excipients are required to achieve stable formulations of protein drug candidates. Synthetic glycopolymers have been shown in some cases to improve protein formulation stability, although their structure function relationship remains unkno... Read More about Synthetic glycopolymers as modulators of protein aggregation: influence of chemical composition, topology and concentration.

Control of targeting ligand display by pH-responsive polymers on gold nanoparticles mediates selective entry into cancer cells (2017)
Journal Article
Brazzale, C., Mastrotto, F., Moody, P., Watson, P., Balasso, A., Malfanti, A., …Salmaso, S. (in press). Control of targeting ligand display by pH-responsive polymers on gold nanoparticles mediates selective entry into cancer cells. Nanoscale, 9(31), https://doi.org/10.1039/C7NR02595E

Selective targeting of cells for intracellular delivery of therapeutics represents a major challenge for pharmaceutical intervention in disease. Here we show pH triggered receptor-mediated endocytosis of nanoparticles via surface ligand exposure. Gol... Read More about Control of targeting ligand display by pH-responsive polymers on gold nanoparticles mediates selective entry into cancer cells.

Polymers for binding of the gram-positive oral pathogen Streptococcus mutans (2017)
Journal Article
Magennis, E. P., Francini, N., Mastrotto, F., Catania, R., Redhead, M., Fernandez-Trillo, F., …Mantovani, G. (2017). Polymers for binding of the gram-positive oral pathogen Streptococcus mutans. PLoS ONE, 12(7), Article e018008. https://doi.org/10.1371/journal.pone.0180087

Streptococcus mutans is the most significant pathogenic bacterium implicated in the formation of dental caries and, both directly and indirectly, has been associated with severe conditions such as multiple sclerosis, cerebrovascular and peripheral ar... Read More about Polymers for binding of the gram-positive oral pathogen Streptococcus mutans.

Effect of polymer topology on non-covalent polymer-protein complexation: miktoarm versus linear mPEG-poly(glutamic acid) copolymers (2017)
Journal Article
Nieto-Orellana, A., Di Antonio, M., Conte, C., Falcone, F. H., Bosquillon, C., Childerhouse, N., …Stolnik, S. (in press). Effect of polymer topology on non-covalent polymer-protein complexation: miktoarm versus linear mPEG-poly(glutamic acid) copolymers. Polymer Chemistry, 8(14), https://doi.org/10.1039/C7PY00169J

Non-covalent polymer-protein conjugation is emerging as a potential route to improve pharmacokinetics and pharmacodynamics of protein therapeutics. In this study, a family of structurally related block copolymers of mPEG2k - poly(glutamic acid) with... Read More about Effect of polymer topology on non-covalent polymer-protein complexation: miktoarm versus linear mPEG-poly(glutamic acid) copolymers.

Phosphonium polymers for gene delivery (2016)
Journal Article
Loczenski Rose, V., Mastrotto, F., & Mantovani, G. (in press). Phosphonium polymers for gene delivery. Polymer Chemistry, 8, https://doi.org/10.1039/C6PY01855F

Phosphonium salt-containing polymers have very recently started to emerge as attractive materials for the engineering non-viral gene delivery systems. Compared to more frequently utilised ammonium-based polymers, some of these materials can enhance b... Read More about Phosphonium polymers for gene delivery.

Amphiphilic block copolymers from a renewable ?-decalactone monomer: prediction and characterization of micellar core effects on drug encapsulation and release (2016)
Journal Article
Kakde, D., Taresco, V., Bansal, K. K., Magennis, E. P., Howdle, S. M., Mantovani, G., …Alexander, C. (in press). Amphiphilic block copolymers from a renewable Ɛ-decalactone monomer: prediction and characterization of micellar core effects on drug encapsulation and release. Journal of Materials Chemistry B, 44, https://doi.org/10.1039/C6TB01839D

Here we describe a methoxy poly(ethyleneglycol)-b-poly(ε-decalactone) (mPEG-b-PεDL) copolymer and investigate the potential of the copolymer as a vehicle for solubilisation and sustained release of indomethacin (IND). The indomethacin loading and rel... Read More about Amphiphilic block copolymers from a renewable ?-decalactone monomer: prediction and characterization of micellar core effects on drug encapsulation and release.

One-pot RAFT and fast polymersomes assembly: a ‘beeline’ from monomers to drug-loaded nanovectors (2016)
Journal Article
Mastrotto, F., Breen, A. F., Sicilia, G., Murdan, S., Johnstone, A. D., Marsh, G. E., …Mantovani, G. (2016). One-pot RAFT and fast polymersomes assembly: a ‘beeline’ from monomers to drug-loaded nanovectors. Polymer Chemistry, 7(44), 6714-6724. https://doi.org/10.1039/C6PY01292B

Rapid and simple routes to functional polymersomes are increasingly needed to expand their clinical or industrial applications. Here we describe a novel strategy where polymersomes are prepared through an in-line process in just a few hours, starting... Read More about One-pot RAFT and fast polymersomes assembly: a ‘beeline’ from monomers to drug-loaded nanovectors.

Synthesis, characterization and evaluation of in vitro toxicity in hepatocytes of linear polyesters with varied aromatic and aliphatic co-monomers (2016)
Journal Article
Kakde, D., Powell, L. G., Bansal, K. K., Howdle, S., Irvine, D., Mantovani, G., …Alexander, C. (2016). Synthesis, characterization and evaluation of in vitro toxicity in hepatocytes of linear polyesters with varied aromatic and aliphatic co-monomers. Journal of Controlled Release, 244(B), https://doi.org/10.1016/j.jconrel.2016.08.003

Polyesters are extensively used in drug delivery because of their controllable biodegradation properties and perceived favorable cytocompatibility. However, new ester-based materials are continually being sought which can be produced from readily acc... Read More about Synthesis, characterization and evaluation of in vitro toxicity in hepatocytes of linear polyesters with varied aromatic and aliphatic co-monomers.

Properties of acyl modified poly(glycerol-adipate) comb-like polymers and their self-assembly into nanoparticles (2016)
Journal Article
Taresco, V., Suksiriworapong, J., Creasey, R., Burley, J. C., Mantovani, G., Alexander, C., & Garnett, M. (2016). Properties of acyl modified poly(glycerol-adipate) comb-like polymers and their self-assembly into nanoparticles. Journal of Polymer Science Part A: Polymer Chemistry, 54(20), 3267-3278. https://doi.org/10.1002/pola.28215

There is an increasing need to develop bio-compatible polymers with an increased range of different physicochemical properties. Poly(glycerol-adipate) (PGA) is a biocompatible, biodegradable amphiphilic polyester routinely produced from divinyl adipa... Read More about Properties of acyl modified poly(glycerol-adipate) comb-like polymers and their self-assembly into nanoparticles.

Variation in structure and properties of poly(glycerol adipate) via control of chain branching during enzymatic synthesis (2016)
Journal Article
Taresco, V., Creasey, R., Kennon, J., Mantovani, G., Alexander, C., Burley, J. C., & Garnett, M. C. (2016). Variation in structure and properties of poly(glycerol adipate) via control of chain branching during enzymatic synthesis. Polymer, 89, https://doi.org/10.1016/j.polymer.2016.02.036

Poly (glycerol adipate) (PGA) can be produced from divinyl adipate and unprotected glycerol by an enzymatic route to generate a polymer with relatively low molar mass (12 kDa). PGA bears a pendant hydroxyl group which imparts a hydrophilic character... Read More about Variation in structure and properties of poly(glycerol adipate) via control of chain branching during enzymatic synthesis.

Phosphonium polymethacrylates for siRNA delivery: effect of polymer and RNA structural parameters on polyplex assembly and gene knockdown (2015)
Journal Article
Loczenski Rose, V., Shubber, S., Sajeesh, S., Spain, S. G., Puri, S., Allen, S., …Mantovani, G. (2015). Phosphonium polymethacrylates for siRNA delivery: effect of polymer and RNA structural parameters on polyplex assembly and gene knockdown. Biomacromolecules, 16(11), https://doi.org/10.1021/acs.biomac.5b00898

Synthetic polymers containing quaternary phosphonium salts are an emerging class of materials for the delivery of oligo/polynucleotides. In this work, cationic phosphonium salt-containing polymethacrylates –and their corresponding ammonium analogues–... Read More about Phosphonium polymethacrylates for siRNA delivery: effect of polymer and RNA structural parameters on polyplex assembly and gene knockdown.

New biomaterials from renewable resources – amphiphilic block copolymers from δ-decalactone (2015)
Journal Article
Bansal, K. K., Kakde, D., Purdie, L., Irvine, D. J., Howdle, S. M., Mantovani, G., & Alexander, C. (2015). New biomaterials from renewable resources – amphiphilic block copolymers from δ-decalactone. Polymer Chemistry, 6(40), https://doi.org/10.1039/C5PY01203A

Triblock copolymer nanovesicles for pH-responsive targeted delivery and controlled release of siRNA to cancer cells (2015)
Journal Article
Gallon, E., Matini, T., Sasso, L., Mantovani, G., Armiñan de Benito, A., Sanchis, J., …Salmaso, S. (2015). Triblock copolymer nanovesicles for pH-responsive targeted delivery and controlled release of siRNA to cancer cells. Biomacromolecules, 16(7), https://doi.org/10.1021/acs.biomac.5b00286

New pH-responsive polymersomes for active anticancer oligonucleotide delivery were prepared from triblock copolymers. The delivery systems were formed by two terminal hydrophilic blocks, PEG and polyglycerolmethacrylate (poly-GMA), and a central weak... Read More about Triblock copolymer nanovesicles for pH-responsive targeted delivery and controlled release of siRNA to cancer cells.

Multifunctional poly[N-(2-hydroxypropyl)methacrylamide] copolymers via postpolymerization modification and sequential thiol–ene chemistry (2015)
Journal Article
Francini, N., Purdie, L., Alexander, C., Mantovani, G., & Spain, S. G. (2015). Multifunctional poly[N-(2-hydroxypropyl)methacrylamide] copolymers via postpolymerization modification and sequential thiol–ene chemistry. Macromolecules, 48(9), https://doi.org/10.1021/acs.macromol.5b00447

Poly[N-(2-hydroxypropyl)methacrylamide] is a promising candidate material for biomedical applications. However, synthesis of functional pHPMA via compolymerization results can lead to variations in monomer composition, molar mass, and dispersity maki... Read More about Multifunctional poly[N-(2-hydroxypropyl)methacrylamide] copolymers via postpolymerization modification and sequential thiol–ene chemistry.

Bacteria-instructed synthesis of polymers for self-selective microbial binding and labelling (2014)
Journal Article
Magennis, E. P., Fernandez-Trillo, F., Sui, C., Spain, S. G., Bradshaw, D., Churchley, D., …Alexander, C. (2014). Bacteria-instructed synthesis of polymers for self-selective microbial binding and labelling. Nature Materials, 13(7), 748-755. https://doi.org/10.1038/nmat3949

The detection and inactivation of pathogenic strains of bacteria continues to be an important therapeutic goal. Hence, there is a need for materials that can bind selectively to specific microorganisms for diagnostic or anti-infective applications, b... Read More about Bacteria-instructed synthesis of polymers for self-selective microbial binding and labelling.