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3D microfabricated scaffolds and microfluidic devices for ocular surface replacement: a review (2017)
Journal Article
Prina, E., Mistry, P., Sidney, L. E., Yang, J., Wildman, R. D., Bertolin, M., …Rose, F. R. A. (2017). 3D microfabricated scaffolds and microfluidic devices for ocular surface replacement: a review. Stem Cell Reviews and Reports, 13(3), 430-441. https://doi.org/10.1007/s12015-017-9740-6

In recent years, there has been increased research interest in generating corneal substitutes, either for use in the clinic or as in vitro corneal models. The advancement of 3D microfabrication technologies has allowed the reconstruction of the nativ... Read More about 3D microfabricated scaffolds and microfluidic devices for ocular surface replacement: a review.

Cultivation and characterisation of the surface markers and carbohydrate profile of human corneal endothelial cells (2016)
Journal Article
Nagymihály, R., Veréb, Z., Albert, R., Sidney, L., Dua, H., Hopkinson, A., & Petrovski, G. (2017). Cultivation and characterisation of the surface markers and carbohydrate profile of human corneal endothelial cells. Clinical and Experimental Ophthalmology, 45(5), 509-519. https://doi.org/10.1111/ceo.12903

Background The study aims to characterise human corneal endothelial cell (HCEnC) cultures generated by the peel?and?digest method based on their surface protein/carbohydrate expression pattern. Methods Quantitative polymerase chain reaction was... Read More about Cultivation and characterisation of the surface markers and carbohydrate profile of human corneal endothelial cells.

Scrolling Characteristics of Pre-Descemet Endothelial Keratoplasty Tissue: An Ex Vivo Study (2016)
Journal Article
Dua, H., Termote, K., Kenawy, M., Said, D., Jayaswal, R., Nubile, M., …Holland, S. (2016). Scrolling Characteristics of Pre-Descemet Endothelial Keratoplasty Tissue: An Ex Vivo Study. American Journal of Ophthalmology, 166, 84-90. https://doi.org/10.1016/j.ajo.2016.03.034

Purpose To evaluate the scrolling propensity of pre-Descemet endothelial keratoplasty (PDEK) tissue and to compare it with each component of the PDEK tissue, namely the pre-Descemet layer (Dua's layer [PDL]) and the Descemet membrane (DM). Desi... Read More about Scrolling Characteristics of Pre-Descemet Endothelial Keratoplasty Tissue: An Ex Vivo Study.

Effect of culture medium on propagation and phenotype of corneal stroma-derived stem cells (2015)
Journal Article
Sidney, L. E., Branch, M. J., Dua, H. S., & Hopkinson, A. (2015). Effect of culture medium on propagation and phenotype of corneal stroma-derived stem cells. Cytotherapy, 17(12), https://doi.org/10.1016/j.jcyt.2015.08.003

Background: The limbal area of the corneal stroma has been identified as a source of mesenchymal-like stem cells, which have potential for exploitation as a cell therapy. However, the optimal culture conditions are disputed and few direct media compa... Read More about Effect of culture medium on propagation and phenotype of corneal stroma-derived stem cells.

Corneal Decellularization: A Method of Recycling Unsuitable Donor Tissue for Clinical Translation? (2015)
Journal Article
Wilson, S. L., Sidney, L. E., Dunphy, S. E., Dua, H. S., & Hopkinson, A. (2016). Corneal Decellularization: A Method of Recycling Unsuitable Donor Tissue for Clinical Translation?. Current Eye Research, 41(6), 769-782. https://doi.org/10.3109/02713683.2015.1062114

Background: There is a clinical need for biomimetic corneas that are as effective, preferably superior, to cadaveric donor tissue. Decellularized tissues are advantageous compared to synthetic or semi-synthetic engineered tissues in that the native m... Read More about Corneal Decellularization: A Method of Recycling Unsuitable Donor Tissue for Clinical Translation?.

Clinical evaluation and characterisation of corneal vascularisation (2015)
Journal Article
Faraj, L., Said, D. G., AL AQABA, M., Otri, A. M., & Dua, H. S. (2016). Clinical evaluation and characterisation of corneal vascularisation. British Journal of Ophthalmology, 100(3), 315-322. doi:10.1136/bjophthalmol-2015-306686

Background/aims: To clinically characterise corneal neovascularisation (CVas) with a view to elaborate clinical presentations and to standardise descriptors for clinical evaluation and research. Methods: Corneas of 165 patients with CVas due to a va... Read More about Clinical evaluation and characterisation of corneal vascularisation.

Calcified corneal nerves (2015)
Journal Article
Faraj, L., Messina, M., AL AQABA, M., Elalfy, M. S., Ghoz, N., & Dua, H. S. (2015). Calcified corneal nerves. Cornea, 34(6), 707-709. doi:10.1097/ICO.0000000000000430

In vitro studies on the antimicrobial peptide human beta-defensin 9 (HBD9): signalling pathways and pathogen-related response (an American Ophthalmological Society thesis) (2014)
Journal Article
Dua, H. S., Otri, A. M., Hopkinson, A., & Mohammed, I. (2014). In vitro studies on the antimicrobial peptide human beta-defensin 9 (HBD9): signalling pathways and pathogen-related response (an American Ophthalmological Society thesis). Transactions of the American Ophthalmological Society, 112, 50-73

Purpose: Human β-defensins (HBDs) are an important part of the innate immune host defense at the ocular surface. Unlike other defensins, expression of HBD9 at the ocular surface is reduced during microbial infection, but activation of toll-like recep... Read More about In vitro studies on the antimicrobial peptide human beta-defensin 9 (HBD9): signalling pathways and pathogen-related response (an American Ophthalmological Society thesis).

Pre-Descemet's endothelial keratoplasty (PDEK) (2014)
Journal Article
Dua, H., Agarwal, A., Narang, P., Kumar, D. A., Agarwal, A., Jacob, S., …Gupta, A. (2014). Pre-Descemet's endothelial keratoplasty (PDEK). British Journal of Ophthalmology, 98(9), 1181-1185. https://doi.org/10.1136/bjophthalmol-2013-304639

A new surgical technique for endothelial keratoplasty is described, in which the composite of pre-Descemet's layer (Dua's layer) with Descemet's membrane and endothelium is transplanted subsequent to the removal of the recipient's Descemet's membrane... Read More about Pre-Descemet's endothelial keratoplasty (PDEK).

Anaesthetic corneas with intact sub-basal nerve plexus (2014)
Journal Article
Al Aqaba, M., Dhillon, V. K., Elalfy, M. S., & Dua, H. S. (2014). Anaesthetic corneas with intact sub-basal nerve plexus. British Journal of Ophthalmology, 98(3), 417-418. doi:10.1136/bjophthalmol-2013-303890

Collagen cross-linking with photoactivated riboflavin (PACK-CXL) for the treatment of advanced infectious keratitis with corneal melting (2014)
Journal Article
Dua, H., Said, D., Elalfy, M., Gatzioufas, Z., El-Zakzouk, E., Hassan, M., …Hafezi, F. (2014). Collagen cross-linking with photoactivated riboflavin (PACK-CXL) for the treatment of advanced infectious keratitis with corneal melting. Ophthalmology: Journal of The American Academy of Ophthalmology, 121(7), 1377-1382. https://doi.org/10.1016/j.ophtha.2014.01.011

Concise review: evidence for CD34 as a common marker for diverse progenitors (2014)
Journal Article
Sidney, L. E., Branch, M. J., Dunphy, S. E., Dua, H. S., & Hopkinson, A. (2014). Concise review: evidence for CD34 as a common marker for diverse progenitors. STEM CELLS, 32(6), 1380-1389. https://doi.org/10.1002/stem.1661

CD34 is a transmembrane phosphoglycoprotein, first identified on hematopoietic stem and progenitor cells. Clinically, it is associated with the selection and enrichment of hematopoietic stem cells for bone marrow transplants. Due to these historical... Read More about Concise review: evidence for CD34 as a common marker for diverse progenitors.

Optimised laser microdissection of the human ocular surface epithelial regions for microarray studies (2013)
Journal Article
Kulkarn, B. B. .., Powe, D. G., Hopkinson, A., & Dua, H. S. (2013). Optimised laser microdissection of the human ocular surface epithelial regions for microarray studies. BMC Ophthalmology, 13, Article 62. https://doi.org/10.1186/1471-2415-13-62

Background The most important challenge of performing insitu transcriptional profiling of the human ocular surface epithelial regions is obtaining samples in sufficient amounts, without contamination from adjacent tissue, as the region of interest i... Read More about Optimised laser microdissection of the human ocular surface epithelial regions for microarray studies.

Corneal Densitometry as an Indicator of Corneal Health (2011)
Journal Article
Otri, A. M., Fares, U., Al-Aqaba, M. A., & Dua, H. S. (2012). Corneal Densitometry as an Indicator of Corneal Health. Ophthalmology: Journal of The American Academy of Ophthalmology, 119(3), 501-508. doi:10.1016/j.ophtha.2011.08.024

Purpose To establish prospectively the normal values of corneal density of healthy subjects using the Pentacam Scheimpflug system (Oculus, Inc., Wetzlar, Germany) and to investigate alteration in corneal density during active and healed stages of ba... Read More about Corneal Densitometry as an Indicator of Corneal Health.

Increased Expression of Hepcidin and Toll-Like Receptors 8 and 10 in Viral Keratitis (2011)
Journal Article
Mohammed, I., Abedin, A., Tsintzas, K., Abedin, S. A., Otri, A. M., Hopkinson, A., …Dua, H. S. (2011). Increased Expression of Hepcidin and Toll-Like Receptors 8 and 10 in Viral Keratitis. Cornea, 30(8), 899-904. https://doi.org/10.1097/ICO.0b013e31820126e5

Purpose: Antimicrobial peptides (AMPs) and toll-like receptors (TLRs) form part of the “chemical barrier” of the ocular surface to microbes. Evidence suggests that pathogen recognition by TLR releases AMPs, altering AMP-TLR profiles in pathologic... Read More about Increased Expression of Hepcidin and Toll-Like Receptors 8 and 10 in Viral Keratitis.

Signalling pathways involved in ribonuclease-7 expression (2010)
Journal Article
Mohammed, I., Yeung, A., Abedin, A., Hopkinson, A., & Dua, H. S. (2011). Signalling pathways involved in ribonuclease-7 expression. Cellular and Molecular Life Sciences, 68(11), 1941-1952. https://doi.org/10.1007/s00018-010-0540-2

Antimicrobial peptides are host defence molecules that play a potential role in preventing infection at the epithelial surfaces. Ribonuclease (RNase)-7 has been shown to possess a broad spectrum of microbicidal activity against various pathogens. Her... Read More about Signalling pathways involved in ribonuclease-7 expression.