All Outputs (171)

Comparative hydrodynamic and nanoscale imaging study on the interactions of teicoplanin-A2 and bovine submaxillary mucin as a model ocular mucin (2023)
Journal Article
Chun, T., Pattem, J., Gillis, R. B., Dinu, V. T., Yakubov, G. E., Corfield, A. P., & Harding, S. E. (2023). Comparative hydrodynamic and nanoscale imaging study on the interactions of teicoplanin-A2 and bovine submaxillary mucin as a model ocular mucin. Scientific Reports, 13, Article 11367. https://doi.org/10.1038/s41598-023-38036-6

Glycopeptide antibiotics are regularly used in ophthalmology to treat infections of Gram-positive bacteria. Aggregative interactions of antibiotics with mucins however can lead to long exposure and increases the risk of resistant species. This study... Read More about Comparative hydrodynamic and nanoscale imaging study on the interactions of teicoplanin-A2 and bovine submaxillary mucin as a model ocular mucin.

Bayesian and ultrasonic sensor aided multi-objective optimisation for sustainable clean-in-place processes (2023)
Journal Article
Bowler, A. L., Rodgers, S., Cook, D. J., & Watson, N. J. (2023). Bayesian and ultrasonic sensor aided multi-objective optimisation for sustainable clean-in-place processes. Food and Bioproducts Processing, 141, 23-35. https://doi.org/10.1016/j.fbp.2023.06.010

In food and drink manufacturing, clean-in-place procedures are essential for hygienic and efficient operations but often over-clean process equipment leading to unnecessary use of energy, water, and chemicals. Previous attempts in the literature to o... Read More about Bayesian and ultrasonic sensor aided multi-objective optimisation for sustainable clean-in-place processes.

Engineering nanowires in bacteria to elucidate electron transport structural–functional relationships (2023)
Journal Article
Myers, B., Catrambone, F., Allen, S., Hill, P. J., Kovacs, K., & Rawson, F. J. (2023). Engineering nanowires in bacteria to elucidate electron transport structural–functional relationships. Scientific Reports, 13(1), Article 8843. https://doi.org/10.1038/s41598-023-35553-2

Bacterial pilin nanowires are protein complexes, suggested to possess electroactive capabilities forming part of the cells’ bioenergetic programming. Their role is thought to be linked to facilitating electron transfer between cells and the external... Read More about Engineering nanowires in bacteria to elucidate electron transport structural–functional relationships.

Predicting bioactivity of antibiotic metabolites by molecular docking and dynamics (2023)
Journal Article
Chio, H., Guest, E. E., Hobman, J. L., Dottorini, T., Hirst, J. D., & Stekel, D. J. (2023). Predicting bioactivity of antibiotic metabolites by molecular docking and dynamics. Journal of Molecular Graphics and Modelling, 123, Article 108508. https://doi.org/10.1016/j.jmgm.2023.108508

Antibiotics enter the environment through waste streams, where they can exert selective pressure for antimicrobial resistance in bacteria. However, many antibiotics are excreted as partly metabolized forms, or can be subject to partial breakdown in w... Read More about Predicting bioactivity of antibiotic metabolites by molecular docking and dynamics.

Protein-to-structure pipeline for ambient-temperature in situ crystallography at VMXi (2023)
Journal Article
Mikolajek, H., Sanchez-Weatherby, J., Sandy, J., Gildea, R. J., Campeotto, I., Cheruvara, H., …Hough, M. A. (2023). Protein-to-structure pipeline for ambient-temperature in situ crystallography at VMXi. IUCrJ, 10(4), 420-429. https://doi.org/10.1107/s2052252523003810

The utility of X-ray crystal structures determined under ambient-temperature conditions is becoming increasingly recognized. Such experiments can allow protein dynamics to be characterized and are particularly well suited to challenging protein targe... Read More about Protein-to-structure pipeline for ambient-temperature in situ crystallography at VMXi.

Evolution: The great photosynthesis heist (2023)
Journal Article
Howe, C. J., & Nisbet, R. E. R. (2023). Evolution: The great photosynthesis heist. Current Biology, 33(5), R185-R187. https://doi.org/10.1016/j.cub.2023.01.030

Many eukaryotes acquired chloroplasts by endosymbiotic acquisition of photosynthetic bacteria or already-domesticated chloroplasts from other eukaryotes. However, the ciliate Mesodinium rubrum acquires the nucleus of a photosynthetic eukaryote, as we... Read More about Evolution: The great photosynthesis heist.

Evaluation of two terpene-derived polymers as consolidants for archaeological wood (2023)
Journal Article
Cutajar, M., Braovac, S., Stockman, R. A., Howdle, S. M., & Harding, S. E. (2023). Evaluation of two terpene-derived polymers as consolidants for archaeological wood. Scientific Reports, 13(1), Article 3664. https://doi.org/10.1038/s41598-023-29785-5

The evaluation of two terpene-derived polymers, termed TPA6 and TPA7, as possible consolidants for archaeological wood was carried out. The overall objective of this work was to expand the non-aqueous treatment toolkit which is available for the cons... Read More about Evaluation of two terpene-derived polymers as consolidants for archaeological wood.

25 years of Protist: A thank you to Editor-in-Chief Michael Melkonian (2023)
Journal Article
Nisbet, R. E. R., & Howe, C. J. (2023). 25 years of Protist: A thank you to Editor-in-Chief Michael Melkonian. Protist, 174(2), Article 125947. https://doi.org/10.1016/j.protis.2023.125947

Understanding Early Modern Beer: An Interdisciplinary Case-Study (2023)
Journal Article
Flavin, S., Meltonville, M., Taverner, C., Reid, J., Lawrence, S., Belloch-Molina, C., & Morrissey, J. (2023). Understanding Early Modern Beer: An Interdisciplinary Case-Study. Historical Journal, 66(3), 516-549. https://doi.org/10.1017/S0018246X23000043

Beer was a staple of early modern diets across northern Europe and the Atlantic World. While its profound social, economic, and cultural significance is well established, little is known about the nature and quality of the drink itself, particularly... Read More about Understanding Early Modern Beer: An Interdisciplinary Case-Study.

Galacto-oligosaccharides fed during gestation increase Rotavirus A specific antibodies in sow colostrum, modulate the microbiome, and reduce infectivity in neonatal piglets in a commercial farm setting (2023)
Journal Article
Lee, A., Liang, L., Connerton, P. L., Connerton, I. F., & Mellits, K. H. (2023). Galacto-oligosaccharides fed during gestation increase Rotavirus A specific antibodies in sow colostrum, modulate the microbiome, and reduce infectivity in neonatal piglets in a commercial farm setting. Frontiers in Veterinary Science, 10, Article 1118302. https://doi.org/10.3389/fvets.2023.1118302

Introduction: Rotavirus A is a major cause of acute dehydrating diarrhea in neonatal pigs resulting in significant mortality, morbidity, reduced performance and economic loss. Commercially available prebiotic galacto-oligosaccharides are similar to t... Read More about Galacto-oligosaccharides fed during gestation increase Rotavirus A specific antibodies in sow colostrum, modulate the microbiome, and reduce infectivity in neonatal piglets in a commercial farm setting.

eIF4A1-dependent mRNAs employ purine-rich 5'UTR sequences to activate localised eIF4A1-unwinding through eIF4A1-multimerisation to facilitate translation (2023)
Journal Article
Schmidt, T., Dabrowska, A., Waldron, J. A., Hodge, K., Koulouras, G., Gabrielsen, M., …Bushell, M. (2023). eIF4A1-dependent mRNAs employ purine-rich 5'UTR sequences to activate localised eIF4A1-unwinding through eIF4A1-multimerisation to facilitate translation. Nucleic Acids Research, 51(4), 1859-1879. https://doi.org/10.1093/nar/gkad030

Altered eIF4A1 activity promotes translation of highly structured, eIF4A1-dependent oncogene mRNAs at root of oncogenic translational programmes. It remains unclear how these mRNAs recruit and activate eIF4A1 unwinding specifically to facilitate thei... Read More about eIF4A1-dependent mRNAs employ purine-rich 5'UTR sequences to activate localised eIF4A1-unwinding through eIF4A1-multimerisation to facilitate translation.

Multidrug-Resistant ESBL-Producing E. coli in Clinical Samples from the UK (2023)
Journal Article
Ibrahim, D. R., Dodd, C. E. R., Stekel, D. J., Meshioye, R. T., Diggle, M., Lister, M., & Hobman, J. L. (2023). Multidrug-Resistant ESBL-Producing E. coli in Clinical Samples from the UK. Antibiotics, 12(1), Article 169. https://doi.org/10.3390/antibiotics12010169

Globally, cephalosporin therapy failure is a serious problem for infection control. One causative agent of cephalosporin-resistant infections is multidrug-resistant (MDR) E. coli producing extended-spectrum β-lactamases (ESBLs) and/or plasmid-encoded... Read More about Multidrug-Resistant ESBL-Producing E. coli in Clinical Samples from the UK.

Establishment of a novel set of vectors for transformation of the dinoflagellate Amphidinium carterae (2022)
Preprint / Working Paper
Nimmo, I., Geisler, K., Barbrook, A., Kleiner, F., Scarampi, A., Kosmützky, D., …Howe, C. Establishment of a novel set of vectors for transformation of the dinoflagellate Amphidinium carterae

Peridinin-containing dinoflagellate algae have a chloroplast genome formed from plasmid-like minicircles. This fragmented genome has allowed us to develop a genetic modification methodology involving the use of biolistics to introduce artificial mini... Read More about Establishment of a novel set of vectors for transformation of the dinoflagellate Amphidinium carterae.

Comparative hydrodynamic characterisation of two hydroxylated polymers based on α-pinene- or oleic acid-derived monomers for potential use as archaeological consolidants (2022)
Journal Article
Cutajar, M., Machado, F., Cuzzucoli Crucitti, V., Braovac, S., Stockman, R. A., Howdle, S. M., & Harding, S. E. (2022). Comparative hydrodynamic characterisation of two hydroxylated polymers based on α-pinene- or oleic acid-derived monomers for potential use as archaeological consolidants. Scientific Reports, 12(1), Article 18411. https://doi.org/10.1038/s41598-022-21027-4

The Oseberg Viking ship burial is one of the most extensive collections of Viking wooden artefacts ever excavated in Norway. In the early twentieth century, many of these artefacts were treated with alum in order to preserve them, inadvertently leadi... Read More about Comparative hydrodynamic characterisation of two hydroxylated polymers based on α-pinene- or oleic acid-derived monomers for potential use as archaeological consolidants.

Synthetic Biology Toolbox, Including a Single-Plasmid CRISPR-Cas9 System to Biologically Engineer the Electrogenic, Metal-Resistant Bacterium Cupriavidus metallidurans CH34 (2022)
Journal Article
Turco, F., Garavaglia, M., Van Houdt, R., Hill, P., Rawson, F. J., & Kovacs, K. (2022). Synthetic Biology Toolbox, Including a Single-Plasmid CRISPR-Cas9 System to Biologically Engineer the Electrogenic, Metal-Resistant Bacterium Cupriavidus metallidurans CH34. ACS Synthetic Biology, https://doi.org/10.1021/acssynbio.2c00130

Cupriavidus metallidurans CH34 exhibits extraordinary metabolic versatility, including chemolithoautotrophic growth; degradation of BTEX (benzene, toluene, ethylbenzene, xylene); high resistance to numerous metals; biomineralization of gold, platinum... Read More about Synthetic Biology Toolbox, Including a Single-Plasmid CRISPR-Cas9 System to Biologically Engineer the Electrogenic, Metal-Resistant Bacterium Cupriavidus metallidurans CH34.

Gut transcriptome reveals differential gene expression and enriched pathways linked to immune activation in response to weaning in pigs (2022)
Journal Article
Le Bon, M., Tötemeyer, S., Emes, R. D., & Mellits, K. H. (2022). Gut transcriptome reveals differential gene expression and enriched pathways linked to immune activation in response to weaning in pigs. Frontiers in Genetics, 13, Article 961474. https://doi.org/10.3389/fgene.2022.961474

Weaning represents one of the most critical periods in pig production associated with increase in disease risk, reduction in performance and economic loss. Physiological changes faced by piglets during the weaning period have been well characterised,... Read More about Gut transcriptome reveals differential gene expression and enriched pathways linked to immune activation in response to weaning in pigs.

The role of inorganic-phosphate, potassium and magnesium in yeast-flavour formation (2022)
Journal Article
Ribeiro-Filho, N., Linforth, R., Bora, N., Powell, C. D., & Fisk, I. D. (2022). The role of inorganic-phosphate, potassium and magnesium in yeast-flavour formation. Food Research International, 162, Article 112044. https://doi.org/10.1016/j.foodres.2022.112044

Inorganic-phosphate, potassium, and magnesium are key-minerals required for yeast growth, metabolism, and survival, the present work investigated its impact in yeast-flavour formation using a multi-factor experimental design, which was used to genera... Read More about The role of inorganic-phosphate, potassium and magnesium in yeast-flavour formation.

Enhancing Microbial Electron Transfer through Synthetic Biology and Biohybrid Approaches: Part I: Bioelectrochemistry for sustainable energy conversion (2022)
Journal Article
Myers, B., Hill, P., Rawson, F., & Kovacs, K. (2022). Enhancing Microbial Electron Transfer through Synthetic Biology and Biohybrid Approaches: Part I: Bioelectrochemistry for sustainable energy conversion. Johnson Matthey Technology Review, 66(4), 443-454. https://doi.org/10.1595/205651322X16548607638938

Traditional microbial synthesis of chemicals and fuels often rely on energy-rich feedstocks such as glucose, raising ethical concerns as they are directly competing with the food supply. Therefore, it is imperative to develop novel processes that rel... Read More about Enhancing Microbial Electron Transfer through Synthetic Biology and Biohybrid Approaches: Part I: Bioelectrochemistry for sustainable energy conversion.

Non-starch polysaccharides in beer and brewing: A review of their occurrence and significance (2022)
Journal Article
Reid, J. E. S. J., Yakubov, G. E., & Lawrence, S. J. (2024). Non-starch polysaccharides in beer and brewing: A review of their occurrence and significance. Critical Reviews in Food Science and Nutrition, 64, 837-851. https://doi.org/10.1080/10408398.2022.2109585

It has become apparent that beer (both alcoholic and nonalcoholic) contains appreciable amounts of non-starch polysaccharides, a broad subgroup of dietary fiber. It is worth noting that the occurrence of non-starch polysaccharides in alcoholic beer d... Read More about Non-starch polysaccharides in beer and brewing: A review of their occurrence and significance.

The effects of exogenous fibrolytic enzymes on the in vitro generation of xylooligosaccharides and monosaccharides is dependent upon cereal type (2022)
Journal Article
Dale, T., Bedford, M. R., Tucker, G. A., Brameld, J. M., & Parr, T. (2022). The effects of exogenous fibrolytic enzymes on the in vitro generation of xylooligosaccharides and monosaccharides is dependent upon cereal type. Animal Feed Science and Technology, 292, Article 115406. https://doi.org/10.1016/j.anifeedsci.2022.115406

Fibrolytic enzymes are routinely added to non-ruminant livestock feeds to help degrade the non-starch polysaccharide (NSP) contents and thereby improve feed efficiency. This study investigated the range of xylooligosaccharides (XOS) and monosaccharid... Read More about The effects of exogenous fibrolytic enzymes on the in vitro generation of xylooligosaccharides and monosaccharides is dependent upon cereal type.