All Outputs (36)

Stable Platform for Mevalonate Bioproduction from CO2 (2024)
Journal Article
Garavaglia, M., McGregor, C., Bommareddy, R. R., Irorere, V., Arenas, C., Robazza, A., Minton, N. P., & Kovacs, K. (2024). Stable Platform for Mevalonate Bioproduction from CO2. ACS Sustainable Chemistry and Engineering, https://doi.org/10.1021/acssuschemeng.4c03561

Stable production of value-added products using a microbial chassis is pivotal for determining the industrial suitability of the engineered biocatalyst. Microbial cells often lose the multicopy expression plasmids during long-term cultivations. Owing... Read More about Stable Platform for Mevalonate Bioproduction from CO2.

Chromosomal integration of the pSOL1 megaplasmid of Clostridium acetobutylicum for continuous and stable advanced biofuels production (2024)
Journal Article
Ehsaan, M., Yoo, M., Kuit, W., Foulquier, C., Soucaille, P., & Minton, N. P. (2024). Chromosomal integration of the pSOL1 megaplasmid of Clostridium acetobutylicum for continuous and stable advanced biofuels production. Nature Microbiology, 9(7), 1655-1660. https://doi.org/10.1038/s41564-024-01714-w

Biofuel production by Clostridium acetobutylicum is compromised by strain degeneration due to loss of its pSOL1 megaplasmid. Here we used engineering biology to stably integrate pSOL1 into the chromosome together with a synthetic isopropanol pathway.... Read More about Chromosomal integration of the pSOL1 megaplasmid of Clostridium acetobutylicum for continuous and stable advanced biofuels production.

Metabolic engineering of Cupriavidus necator H16 for heterotrophic and autotrophic production of 3-hydroxypropionic acid (2022)
Journal Article
Salinas, A., McGregor, C., Irorere, V., Arenas-López, C., Bommareddy, R. R., Winzer, K., …Kovács, K. (2022). Metabolic engineering of Cupriavidus necator H16 for heterotrophic and autotrophic production of 3-hydroxypropionic acid. Metabolic Engineering, 74, 178-190. https://doi.org/10.1016/j.ymben.2022.10.014

3-Hydroxypropionate (3-HP) is a versatile compound for chemical synthesis and a potential building block for biodegradable polymers. Cupriavidus necator H16, a facultative chemolithoautotroph, is an attractive production chassis and has been extensiv... Read More about Metabolic engineering of Cupriavidus necator H16 for heterotrophic and autotrophic production of 3-hydroxypropionic acid.

Colonisation factor cd0873, an attractive oral vaccine candidate against clostridioides difficile (2021)
Journal Article
Karyal, C., Hughes, J., Kelly, M. L., Cockayne, A., Luckett, J. C., Kaye, P. V., …Griffin, R. (2021). Colonisation factor cd0873, an attractive oral vaccine candidate against clostridioides difficile. Microorganisms, 9(2), Article 306. https://doi.org/10.3390/microorganisms9020306

Clostridioides difficile is the main cause of health-care-associated infectious diarrhoea. Toxins, TcdA and TcdB, secreted by this bacterium damage colonic epithelial cells and in severe cases this culminates in pseudomembranous colitis, toxic megaco... Read More about Colonisation factor cd0873, an attractive oral vaccine candidate against clostridioides difficile.

Variability in Arsenic Methylation Efficiency across Aerobic and Anaerobic Microorganisms (2020)
Journal Article
Viacava, K., Meibom, K. L., Ortega, D., Dyer, S., Gelb, A., Falquet, L., …Bernier-Latmani, R. (2020). Variability in Arsenic Methylation Efficiency across Aerobic and Anaerobic Microorganisms. Environmental Science and Technology, 54(22), 14343-14351. https://doi.org/10.1021/acs.est.0c03908

© 2020 American Chemical Society. Microbially-mediated methylation of arsenic (As) plays an important role in the As biogeochemical cycle, particularly in rice paddy soils where methylated As, generated microbially, is translocated into rice grains.... Read More about Variability in Arsenic Methylation Efficiency across Aerobic and Anaerobic Microorganisms.

Synthetic biology on acetogenic bacteria for highly efficient conversion of c1 gases to biochemicals (2020)
Journal Article
Jin, S., Bae, J., Song, Y., Pearcy, N., Shin, J., Kang, S., …Cho, B. K. (2020). Synthetic biology on acetogenic bacteria for highly efficient conversion of c1 gases to biochemicals. International Journal of Molecular Sciences, 21(20), Article 7639. https://doi.org/10.3390/ijms21207639

© 2020 by the authors. Licensee MDPI, Basel, Switzerland. Synthesis gas, which is mainly produced from fossil fuels or biomass gasification, consists of C1 gases such as carbon monoxide, carbon dioxide, and methane as well as hydrogen. Acetogenic bac... Read More about Synthetic biology on acetogenic bacteria for highly efficient conversion of c1 gases to biochemicals.

The carbonic anhydrase of Clostridium autoethanogenum represents a new subclass of β-carbonic anhydrases (2019)
Journal Article
Pander, B., Harris, G., Scott, D. J., Winzer, K., Köpke, M., Simpson, S. D., …Henstra, A. M. (2019). The carbonic anhydrase of Clostridium autoethanogenum represents a new subclass of β-carbonic anhydrases. Applied Microbiology and Biotechnology, 103(17), 7275-7286. https://doi.org/10.1007/s00253-019-10015-w

Carbonic anhydrase catalyses the interconversion of carbon dioxide and water to bicarbonate and protons. It was unknown if the industrial relevant acetogen Clostridium autoethanogenum possesses these enzymes. We identified two putative carbonic anhyd... Read More about The carbonic anhydrase of Clostridium autoethanogenum represents a new subclass of β-carbonic anhydrases.

Homologous overexpression of hydrogenase and glycerol dehydrogenase in Clostridium pasteurianum to enhance hydrogen production from crude glycerol (2019)
Journal Article
Sarma, S., Ortega, D., Minton, N. P., Dubey, V. K., & Moholkar, V. S. (2019). Homologous overexpression of hydrogenase and glycerol dehydrogenase in Clostridium pasteurianum to enhance hydrogen production from crude glycerol. Bioresource Technology, 284, 168-177. https://doi.org/10.1016/j.biortech.2019.03.074

This study reports engineering of a hypertransformable variant of C. pasteurianum for bioconversion of glycerol into hydrogen (H2). A functional glycerol-triggered hydrogen pathway was engineered based on two approaches: (1) increasing product yield... Read More about Homologous overexpression of hydrogenase and glycerol dehydrogenase in Clostridium pasteurianum to enhance hydrogen production from crude glycerol.

CRISPR-Cas9D10A nickase-assisted base editing in the solvent producer Clostridium beijerinckii (2019)
Journal Article
Li, Q., Seys, F. M., Minton, N. P., Yang, J., Jiang, Y., Jiang, W., & Yang, S. (2019). CRISPR-Cas9D10A nickase-assisted base editing in the solvent producer Clostridium beijerinckii. Biotechnology and Bioengineering, 116(6), 1475-1483. https://doi.org/10.1002/bit.26949

Clostridium beijerinckii is a potentially important industrial microorganism as it can synthesize valuable chemicals and fuels from various carbon sources. The establishment of convenient to use, effective gene tools with which the organism can be ra... Read More about CRISPR-Cas9D10A nickase-assisted base editing in the solvent producer Clostridium beijerinckii.

A transcription factor-based biosensor for detection of itaconic acid (2018)
Journal Article
Hanko, E. K., Minton, N. P., & Malys, N. (2018). A transcription factor-based biosensor for detection of itaconic acid. ACS Synthetic Biology, 7(5), 1436–1446. https://doi.org/10.1021/acssynbio.8b00057

Itaconic acid is an important platform chemical that can easily be incorporated into polymers and has the potential to replace petrochemical-based acrylic or methacrylic acid. A number of microorganisms have been developed for the biosynthesis of ita... Read More about A transcription factor-based biosensor for detection of itaconic acid.

Characterization of the impact of rpoB mutations on the in vitro and in vivo competitive fitness of Clostridium difficile and susceptibility to fidaxomicin (2017)
Journal Article
Kuehne, S. A., Dempster, A. W., Collery, M. M., Joshi, N., Jowett, J., Kelly, M. L., …Minton, N. P. (2018). Characterization of the impact of rpoB mutations on the in vitro and in vivo competitive fitness of Clostridium difficile and susceptibility to fidaxomicin. Journal of Antimicrobial Chemotherapy, 73(4), 973-980. https://doi.org/10.1093/jac/dkx486

Objectives
To establish the role of specific, non-synonymous SNPs in the RNA polymerase β subunit (rpoB) gene in reducing the susceptibility of Clostridium difficile to fidaxomicin and to explore the potential in vivo significance of rpoB mutant str... Read More about Characterization of the impact of rpoB mutations on the in vitro and in vivo competitive fitness of Clostridium difficile and susceptibility to fidaxomicin.

Recombinant expression and characterisation of the oxygen-sensitive 2-enoate reductase from Clostridium sporogenes (2017)
Journal Article
Mordaka, P. M., Hall, S. J., Minton, N. P., & Stephens, G. (in press). Recombinant expression and characterisation of the oxygen-sensitive 2-enoate reductase from Clostridium sporogenes. Microbiology, https://doi.org/10.1099/mic.0.000568

‘Ene’-reductases have attracted significant attention for the preparation of chemical intermediates and biologically active products. To date, research has been focussed primarily on Old Yellow Enzyme-like proteins, due to their ease of handling, whe... Read More about Recombinant expression and characterisation of the oxygen-sensitive 2-enoate reductase from Clostridium sporogenes.

Exposing hidden putative lipoproteins in Clostridium difficile (2017)
Presentation / Conference Contribution
Griffin, R., & Minton, N. P. (2017, August). Exposing hidden putative lipoproteins in Clostridium difficile. Poster presented at ClostPath 10th International Conference on the Molecular Biology and Pathogenesis of the Clostrida, Ann Arbor, Michigan, USA

Microbial solvent formation revisited by comparative genome analysis (2017)
Journal Article
Poehlein, A., Solano, J. D. M., Flitsch, S. K., Krabben, P., Winzer, K., Reid, S. J., …Dürre, P. (2017). Microbial solvent formation revisited by comparative genome analysis. Biotechnology for Biofuels, 10, Article 58. https://doi.org/10.1186/s13068-017-0742-z

Background: Microbial formation of acetone, isopropanol, and butanol is largely restricted to bacteria belonging to the genus Clostridium. This ability has been industrially exploited over the last 100 years. The solvents are important feedstocks for... Read More about Microbial solvent formation revisited by comparative genome analysis.

Towards improved butanol production through targeted genetic modification of Clostridium pasteurianum (2017)
Journal Article
Schwarz, K., Grosse-Honebrink, A., Derecka, K., Rotta, C., Zhang, Y., & Minton, N. P. (2017). Towards improved butanol production through targeted genetic modification of Clostridium pasteurianum. Metabolic Engineering, 40, 124-137. https://doi.org/10.1016/j.ymben.2017.01.009

Declining fossil fuel reserves, coupled with environmental concerns over their continued extraction and exploitation have led to strenuous efforts to identify renewable routes to energy and fuels. One attractive option is to convert glycerol, a by-pr... Read More about Towards improved butanol production through targeted genetic modification of Clostridium pasteurianum.

Enhanced solvent production by metabolic engineering of a twin-clostridial consortium (2016)
Journal Article
Wen, Z., Minton, N. P., Zhang, Y., Li, Q., Liu, J., Jiang, Y., & Yang, S. (2017). Enhanced solvent production by metabolic engineering of a twin-clostridial consortium. Metabolic Engineering, 39, 38-48. https://doi.org/10.1016/j.ymben.2016.10.013

The binary toxin CDT enhances Clostridium difficile virulence by suppressing protective colonic eosinophilia (2016)
Journal Article
Cowardin, C. A., Buonomo, E. L., Saleh, M. M., Wilson, M. G., Burgess, S. L., Schwan, C., …Petri, W. A. (2016). The binary toxin CDT enhances Clostridium difficile virulence by suppressing protective colonic eosinophilia. Nature Microbiology, 1(8), Article 16108. https://doi.org/10.1038/nmicrobiol.2016.108

© 2016 Macmillan Publishers Limited. All rights reserved. Clostridium difficile is the most common hospital acquired pathogen in the USA, and infection is, in many cases, fatal. Toxins A and B are its major virulence factors, but expression of a thir... Read More about The binary toxin CDT enhances Clostridium difficile virulence by suppressing protective colonic eosinophilia.

SBRC-Nottingham: sustainable routes to platform chemicals from C1 wastes Gases (2016)
Journal Article
Burbidge, A., & Minton, N. P. (2016). SBRC-Nottingham: sustainable routes to platform chemicals from C1 wastes Gases. Biochemical Society Transactions, 44(3), 684-686. https://doi.org/10.1042/BST20160010

SBRC-Nottingham (www.sbrc-nottingham.ac.uk) was one of the first three UK university-based Synthetic Biology Research Centres (SBRCs) to be funded by the BBSRC and EPSRC as part of the recommendations made in the UKs Synthetic Biology Roadmap. It was... Read More about SBRC-Nottingham: sustainable routes to platform chemicals from C1 wastes Gases.

A roadmap for gene system development in Clostridium (2016)
Journal Article
Minton, N. P., Ehsaan, M., Humphreys, C. M., Little, G. T., Baker, J., Henstra, A. M., …Zhang, Y. (2016). A roadmap for gene system development in Clostridium. Anaerobe, 41, 104-112. https://doi.org/10.1016/j.anaerobe.2016.05.011

Clostridium species are both heroes and villains. Some cause serious human and animal diseases, those present in the microbiota contribute to health and wellbeing, while others represent useful industrial chassis for the production of chemicals and f... Read More about A roadmap for gene system development in Clostridium.

Insights into CO2 fixation pathway of Clostridium autoethanogenumby targeted mutagenesis (2016)
Journal Article
Liew, F., Henstra, A. M., Winzer, K., Kopke, M., Simpson, S. D., & Minton, N. P. (in press). Insights into CO2 fixation pathway of Clostridium autoethanogenumby targeted mutagenesis. mBio, 7(3), Article e00427-16. https://doi.org/10.1128/mBio.00427-16

The future sustainable production of chemicals and fuels from nonpetrochemical resources and reduction of greenhouse gas emissions are two of the greatest societal challenges. Gas fermentation, which utilizes the ability of acetogenic bacteria such a... Read More about Insights into CO2 fixation pathway of Clostridium autoethanogenumby targeted mutagenesis.