Synthetic biology on acetogenic bacteria for highly efficient conversion of c1 gases to biochemicals

Jin, Sangrak; Bae, Jiyun; Song, Yoseb; Pearcy, Nicole; Shin, Jongoh; Kang, Seulgi; Minton, Nigel P.; Soucaille, Philippe; Cho, Byung Kwan

Abstract

© 2020 by the authors. Licensee MDPI, Basel, Switzerland. Synthesis gas, which is mainly produced from fossil fuels or biomass gasification, consists of C1 gases such as carbon monoxide, carbon dioxide, and methane as well as hydrogen. Acetogenic bacteria (acetogens) have emerged as an alternative solution to recycle C1 gases by converting them into value-added biochemicals using the Wood-Ljungdahl pathway. Despite the advantage of utilizing acetogens as biocatalysts, it is difficult to develop industrial-scale bioprocesses because of their slow growth rates and low productivities. To solve these problems, conventional approaches to metabolic engineering have been applied; however, there are several limitations owing to the lack of required genetic bioparts for regulating their metabolic pathways. Recently, synthetic biology based on genetic parts, modules, and circuit design has been actively exploited to overcome the limitations in acetogen engineering. This review covers synthetic biology applications to design and build industrial platform acetogens.

Citation

Jin, S., Bae, J., Song, Y., Pearcy, N., Shin, J., Kang, S., …Cho, B. K. (2020). Synthetic biology on acetogenic bacteria for highly efficient conversion of c1 gases to biochemicals. International Journal of Molecular Sciences, 21(20), Article 7639. https://doi.org/10.3390/ijms21207639

• Journal Article Type: Article
• Acceptance Date: Oct 13, 2020
• Online Publication Date: Oct 15, 2020
• Publication Date: Oct 15, 2020
• Deposit Date: Nov 3, 2020
• Publicly Available Date: Nov 3, 2020
• Journal: International Journal of Molecular Sciences
• Print ISSN: 1661-6596
• Electronic ISSN: 1422-0067
• Publisher: MDPI
• Peer Reviewed: Peer Reviewed
• Volume: 21
• Issue: 20
• Article Number: 7639
• DOI: https://doi.org/10.3390/ijms21207639
• Keywords: Physical and Theoretical Chemistry; Inorganic Chemistry; Organic Chemistry; Spectroscopy; Molecular Biology; Catalysis; General Medicine; Computer Science Applications
• Public URL: https://nottingham-repository.worktribe.com/output/4974002
• Publisher URL: https://www.mdpi.com/1422-0067/21/20/7639