Alejandro Salinas
Metabolic engineering of Cupriavidus necator H16 for heterotrophic and autotrophic production of 3-hydroxypropionic acid
Salinas, Alejandro; McGregor, Callum; Irorere, Victor; Arenas-López, Christian; Bommareddy, Rajesh Reddy; Winzer, Klaus; Minton, Nigel P.; Kovács, Katalin
Authors
Callum McGregor
Victor Irorere
Christian Arenas-López
Rajesh Reddy Bommareddy
Dr Klaus Winzer klaus.winzer@nottingham.ac.uk
ASSOCIATE PROFESSOR
Nigel P. Minton
Dr KATALIN KOVACS katalin.kovacs@nottingham.ac.uk
ASSOCIATE PROFESSOR
Contributors
Dr KATALIN KOVACS katalin.kovacs@nottingham.ac.uk
Research Group
Abstract
3-Hydroxypropionate (3-HP) is a versatile compound for chemical synthesis and a potential building block for biodegradable polymers. Cupriavidus necator H16, a facultative chemolithoautotroph, is an attractive production chassis and has been extensively studied as a model organism for biopolymer production. Here, we engineered C. necator H16 for 3-HP biosynthesis from its central metabolism. Wild type C. necator H16 can use 3-HP as a carbon source, a highly undesirable trait for a 3-HP production chassis. However, deletion of its three (methyl-)malonate semialdehyde dehydrogenases (mmsA1, mmsA2 and mmsA3) resulted in a strain that cannot grow on 3-HP as the sole carbon source, and this strain was selected as our production host. A stepwise approach was used to construct pathways for 3-HP production via β-alanine. Two additional gene deletion targets were identified during the pathway construction process. Deletion of the 3-hydroxypropionate dehydrogenase, encoded by hpdH, prevented the re-consumption of the 3-HP produced by our engineered strains, while deletion of gdhA1, annotated as a glutamate dehydrogenase, prevented the utilization of aspartate as a carbon source, one of the key pathway intermediates. The final strain carrying these deletions was able to produce up to 8 mM 3-HP heterotrophically. Furthermore, an engineered strain was able to produce 0.5 mM 3-HP under autotrophic conditions, using CO2 as sole carbon source. These results form the basis for establishing C. necator H16 as an efficient platform for the production of 3-HP and 3-HP-containing polymers.
Citation
Salinas, A., McGregor, C., Irorere, V., Arenas-López, C., Bommareddy, R. R., Winzer, K., Minton, N. P., & Kovács, K. (2022). Metabolic engineering of Cupriavidus necator H16 for heterotrophic and autotrophic production of 3-hydroxypropionic acid. Metabolic Engineering, 74, 178-190. https://doi.org/10.1016/j.ymben.2022.10.014
Journal Article Type | Article |
---|---|
Acceptance Date | Oct 30, 2022 |
Online Publication Date | Nov 3, 2022 |
Publication Date | 2022-11 |
Deposit Date | Nov 8, 2022 |
Publicly Available Date | Nov 10, 2022 |
Journal | Metabolic Engineering |
Print ISSN | 1096-7176 |
Electronic ISSN | 1096-7184 |
Publisher | Elsevier |
Peer Reviewed | Peer Reviewed |
Volume | 74 |
Pages | 178-190 |
DOI | https://doi.org/10.1016/j.ymben.2022.10.014 |
Keywords | Applied Microbiology and Biotechnology, Bioengineering, Biotechnology |
Public URL | https://nottingham-repository.worktribe.com/output/13455138 |
Publisher URL | https://www.sciencedirect.com/science/article/pii/S1096717622001331?via%3Dihub |
Files
1-s2.0-S1096717622001331-main
(4.3 Mb)
PDF
Publisher Licence URL
https://creativecommons.org/licenses/by/4.0/
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