Chromosomal integration of the pSOL1 megaplasmid of Clostridium acetobutylicum for continuous and stable advanced biofuels production

Ehsaan, Muhammad; Yoo, Minyeong; Kuit, Wouter; Foulquier, Céline; Soucaille, Philippe; Minton, Nigel P.

Abstract

Biofuel production by Clostridium acetobutylicum is compromised by strain degeneration due to loss of its pSOL1 megaplasmid. Here we used engineering biology to stably integrate pSOL1 into the chromosome together with a synthetic isopropanol pathway. In a membrane bioreactor continuously fed with glucose mineral medium, the final strain produced advanced biofuels, n-butanol and isopropanol, at high yield (0.31 g g−1), titre (15.4 g l−1) and productivity (15.5 g l−1 h−1) without degeneration.

Citation

Ehsaan, M., Yoo, M., Kuit, W., Foulquier, C., Soucaille, P., & Minton, N. P. (2024). Chromosomal integration of the pSOL1 megaplasmid of Clostridium acetobutylicum for continuous and stable advanced biofuels production. Nature Microbiology, 9(7), 1655-1660. https://doi.org/10.1038/s41564-024-01714-w

• Journal Article Type: Article
• Acceptance Date: Apr 24, 2024
• Online Publication Date: Jun 14, 2024
• Publication Date: Jul 1, 2024
• Deposit Date: Jun 28, 2024
• Publicly Available Date: Jun 28, 2024
• Journal: Nature Microbiology
• Electronic ISSN: 2058-5276
• Publisher: Nature Publishing Group
• Peer Reviewed: Peer Reviewed
• Volume: 9
• Issue: 7
• Pages: 1655-1660
• DOI: https://doi.org/10.1038/s41564-024-01714-w
• Keywords: Metabolic engineering
• Public URL: https://nottingham-repository.worktribe.com/output/36307174
• Publisher URL: https://www.nature.com/articles/s41564-024-01714-w
• Additional Information: Received: 30 May 2023; Accepted: 24 April 2024; First Online: 14 June 2024; : The authors declare no competing interests.