Integration of DNA into bacterial chromosomes from plasmids without a counter-selection marker

Heap, John T.; Ehsaan, Muhammad; Cooksley, Clare M.; Ng, Yen-Kuan; Cartman, Stephen T.; Winzer, Klaus; Minton, Nigel P.

doi:10.1093/nar/gkr1321

Effects of Tai Chi exercise on physical and psychological health of older people (2012)
Journal Article

Thermo-mechanical fatigue testing and simulation using a viscoplasticity model for a P91 steel (2012)
Journal Article

An experimental programme of cyclic thermo-mechanical testing for a P91 power plant steel, under isothermal, and in-phase and out-of-phase thermo-mechanical, temperature-strain cycle conditions, has been implemented. Using the experimental data, an o... Read More about Thermo-mechanical fatigue testing and simulation using a viscoplasticity model for a P91 steel.

Saints and lovers: myths of the avant-garde in Michel Georges-Michel's Les Montparnos (2012)
Journal Article

This article examines Michel Georges-Michel’s 1924 novel Les Montparnos as a study of the myths circulating around the Montparnasse avant-garde of the 1920s, and their function in relation to art. Key amongst these myths is the idea of art as a relig... Read More about Saints and lovers: myths of the avant-garde in Michel Georges-Michel's Les Montparnos.

Person centred dementia care: problems and possibilities (2012)
Journal Article

Purpose Dementia care is an issue of increasing policy focus, with person centred approaches becoming synonymous with quality provision in this area. However, the implementation and efficacy of this approach is difficult to measure and there are s... Read More about Person centred dementia care: problems and possibilities.

Semi-quantitative immunohistochemical detection of 5-hydroxymethyl-cytosine reveals conservation of its tissue distribution between amphibians and mammals (2012)
Journal Article

5-Hydroxymethyl-cytosine (5-hmC) is a form of modified cytosine, which has recently attracted a considerable attention due to its potential role in transcriptional regulation. According to several reports 5-hydroxymethyl-cytosine distribution is tiss... Read More about Semi-quantitative immunohistochemical detection of 5-hydroxymethyl-cytosine reveals conservation of its tissue distribution between amphibians and mammals.

135 Smoking and lung cancer in women (2012)
Journal Article

Listening to the Tube Map: Rhythm and the Historiography of Urban Map Use (2012)
Journal Article

This paper is in two parts. In the first half I consider the challenge posed by the recent performative turn in critical cartography to the urban historical geographer. If maps come into being only within the diverse moments of their use, then how ca... Read More about Listening to the Tube Map: Rhythm and the Historiography of Urban Map Use.

Research of Detection and Control System for Lunar Dust Effects Simulator (2012)
Journal Article

To simluate the effects of lunar dust environment veritably by using lunar dust effects simulator, a detection and control system based on singlechip microcomputer was developed. In this system, peripheral circuits with stepper motor driver, temperat... Read More about Research of Detection and Control System for Lunar Dust Effects Simulator.

General second-order scalar-tensor theory and self-tuning (2012)
Journal Article

Starting from the most general scalar-tensor theory with second order field equations in four dimensions, we establish the unique action that will allow for the existence of a consistent self-tuning mechanism on FLRW backgrounds, and show how it can... Read More about General second-order scalar-tensor theory and self-tuning.

The LIMD1 protein bridges an association between the prolyl hydroxylases and VHL to repress HIF-1 activity (2012)
Journal Article

There are three prolyl hydroxylases (PHD1, 2 and 3) that regulate the hypoxia-inducible factors (HIFs), the master transcriptional regulators that respond to changes in intracellular O2 tension1,2. In high O2 tension (normoxia) the PHDs hydroxylate t... Read More about The LIMD1 protein bridges an association between the prolyl hydroxylases and VHL to repress HIF-1 activity.

A European study investigating patterns of transition from home care towards institutional dementia care: The protocol of a RightTimePlaceCare study (2012)
Journal Article

Background: Health care policies in many countries aim to enable people with dementia to live in their own homes as long as possible. However, at some point during the disease the needs of a significant number of people with dementia cannot be approp... Read More about A European study investigating patterns of transition from home care towards institutional dementia care: The protocol of a RightTimePlaceCare study.

Introduction: The documentary film movement and the spaces of British identity (2012)
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Tribbles 2 (Trib2) is a novel regulator of toll-like receptor 5 signaling (2012)
Journal Article

Background: Toll-like receptors (TLRs) are expressed by a variety of cells, including intestinal epithelia. However, the full spectrum of regulators modulating innate responses via TLRs has not been delineated. Tribbles (Trib) have been identified as... Read More about Tribbles 2 (Trib2) is a novel regulator of toll-like receptor 5 signaling.

Coo?peratie in spelshows (2012)
Book Chapter

In this chapter, we argue that game shows can be a useful source of data to study cooperative behavior. The unique combination of well-defined decision problems and large stakes makes game show data complementary to both experimental and field data.... Read More about Coo?peratie in spelshows.

Seroprevalence of vectorborne diseases in free-roaming dogs in Goa, India (2012)
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Protocol for a qualitative study exploring the roles of ‘Diffusion Fellows’ in bridging the research to practice gap in the Nottinghamshire, Derbyshire and Lincolnshire Collaboration for Leadership in Applied Health Research and Care (CLAHRC-NDL) (2012)
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The role of genetics in pre-eclampsia and potential pharmacogenomic interventions (2012)
Journal Article

The pregnancy-specific condition pre-eclampsia not only affects the health of mother and baby during pregnancy but also has long-term consequences, increasing the chances of cardiovascular disease in later life. It is accepted that pre-eclampsia has... Read More about The role of genetics in pre-eclampsia and potential pharmacogenomic interventions.

Subspace learning from image gradient orientations (2012)
Journal Article

We introduce the notion of subspace learning from image gradient orientations for appearance-based object recognition. As image data is typically noisy and noise is substantially different from Gaussian, traditional subspace learning from pixel inten... Read More about Subspace learning from image gradient orientations.

‘Appropriate’ medical treatment: what’s in a word? (2012)
Journal Article

Following the amendments in the 2007 Act, there were several revisions made focusing largely on community treatment orders and deprivation of liberty of persons lacking capacity. One of the amendments included a requirement that ‘appropriate treatmen... Read More about ‘Appropriate’ medical treatment: what’s in a word?.

Integration of DNA into bacterial chromosomes from plasmids without a counter-selection marker (2012)
Journal Article

Most bacteria can only be transformed with circular plasmids, so robust DNA integration methods for these rely upon selection of single-crossover clones followed by counter-selection of double-crossover clones. To overcome the limited availability of... Read More about Integration of DNA into bacterial chromosomes from plasmids without a counter-selection marker.

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