Skip to main content

Research Repository

See what's under the surface

Semi-quantitative immunohistochemical detection of 5-hydroxymethyl-cytosine reveals conservation of its tissue distribution between amphibians and mammals

Almeida, Rimple D.; Sottile, Virginie; Loose, Matthew; De Sousa, Paul A.; Johnson, Andrew D.; Ruzov, Alexey

Authors

Rimple D. Almeida

Virginie Sottile

Matthew Loose

Paul A. De Sousa

Andrew D. Johnson

Alexey Ruzov Alexey.Ruzov@nottingham.ac.uk



Abstract

5-Hydroxymethyl-cytosine (5-hmC) is a form of modified cytosine, which has recently attracted a considerable attention due to its potential role in transcriptional regulation. According to several reports 5-hydroxymethyl-cytosine distribution is tissue-specific in mammals. Thus, 5-hmC is enriched in embryonic cell populations and in adult neuronal tissue. Here, we describe a novel method of semi-quantitative immunohistochemical detection of 5-hmC and utilize it to assess the levels of this modification in amphibian tissues. We show that, similar to mammalian embryos, 5-hmC is enriched in axolotl tadpoles compared with adult tissues. Our data demonstrate that 5-hmC distribution is tissue-specific in amphibians, and that strong 5-hmC enrichment in neuronal cells is conserved between amphibians and mammals. In addition, we identify 5-hmC-enriched cell populations that are distributed in amphibian skin and connective tissue in a mosaic manner. Our results illustrate that immunochemistry can be successfully used not only for spatial identification of cells enriched with 5-hmC, but also for the semi-quantitative assessment of the levels of this epigenetic modification in single cells of different tissues.

Journal Article Type Article
Publication Date Feb 1, 2012
Journal Epigenetics
Print ISSN 1559-2294
Electronic ISSN 1559-2294
Publisher Taylor & Francis
Peer Reviewed Peer Reviewed
Volume 7
Issue 2
Institution Citation Almeida, R. D., Sottile, V., Loose, M., De Sousa, P. A., Johnson, A. D., & Ruzov, A. (2012). Semi-quantitative immunohistochemical detection of 5-hydroxymethyl-cytosine reveals conservation of its tissue distribution between amphibians and mammals. Epigenetics, 7(2), doi:10.4161/epi.7.2.18949
DOI https://doi.org/10.4161/epi.7.2.18949
Publisher URL https://www.landesbioscience.com/journals/epigenetics/article/18949/
Copyright Statement Copyright information regarding this work can be found at the following address: http://creativecommons.org/licenses/by-nc/4.0

Files

AlmeidaEPI7-2.pdf (1.4 Mb)
PDF

Copyright Statement
Copyright information regarding this work can be found at the following address: http://creativecommons.org/licenses/by-nc/4.0



Downloadable Citations