Rimple D. Almeida
Semi-quantitative immunohistochemical detection of 5-hydroxymethyl-cytosine reveals conservation of its tissue distribution between amphibians and mammals
Almeida, Rimple D.; Sottile, Virginie; Loose, Matthew; De Sousa, Paul A.; Johnson, Andrew D.; Ruzov, Alexey
Authors
Virginie Sottile
MATTHEW LOOSE matt.loose@nottingham.ac.uk
Professor of Developmental and Computational Biology
Paul A. De Sousa
Andrew D. Johnson
Alexey Ruzov
Abstract
5-Hydroxymethyl-cytosine (5-hmC) is a form of modified cytosine, which has recently attracted a considerable attention due to its potential role in transcriptional regulation. According to several reports 5-hydroxymethyl-cytosine distribution is tissue-specific in mammals. Thus, 5-hmC is enriched in embryonic cell populations and in adult neuronal tissue. Here, we describe a novel method of semi-quantitative immunohistochemical detection of 5-hmC and utilize it to assess the levels of this modification in amphibian tissues. We show that, similar to mammalian embryos, 5-hmC is enriched in axolotl tadpoles compared with adult tissues. Our data demonstrate that 5-hmC distribution is tissue-specific in amphibians, and that strong 5-hmC enrichment in neuronal cells is conserved between amphibians and mammals. In addition, we identify 5-hmC-enriched cell populations that are distributed in amphibian skin and connective tissue in a mosaic manner. Our results illustrate that immunochemistry can be successfully used not only for spatial identification of cells enriched with 5-hmC, but also for the semi-quantitative assessment of the levels of this epigenetic modification in single cells of different tissues.
Citation
Almeida, R. D., Sottile, V., Loose, M., De Sousa, P. A., Johnson, A. D., & Ruzov, A. (2012). Semi-quantitative immunohistochemical detection of 5-hydroxymethyl-cytosine reveals conservation of its tissue distribution between amphibians and mammals. Epigenetics, 7(2), https://doi.org/10.4161/epi.7.2.18949
Journal Article Type | Article |
---|---|
Publication Date | Feb 1, 2012 |
Deposit Date | Apr 22, 2014 |
Publicly Available Date | Apr 22, 2014 |
Journal | Epigenetics |
Print ISSN | 1559-2294 |
Electronic ISSN | 1559-2294 |
Publisher | Taylor & Francis Open |
Peer Reviewed | Peer Reviewed |
Volume | 7 |
Issue | 2 |
DOI | https://doi.org/10.4161/epi.7.2.18949 |
Public URL | https://nottingham-repository.worktribe.com/output/708917 |
Publisher URL | https://www.landesbioscience.com/journals/epigenetics/article/18949/ |
Files
AlmeidaEPI7-2.pdf
(1.4 Mb)
PDF
Copyright Statement
Copyright information regarding this work can be found at the following address: http://creativecommons.org/licenses/by-nc/4.0
You might also like
Genome Reference Assembly for Bottlenecked Southern Australian Koalas
(2022)
Journal Article
Downloadable Citations
About Repository@Nottingham
Administrator e-mail: digital-library-support@nottingham.ac.uk
This application uses the following open-source libraries:
SheetJS Community Edition
Apache License Version 2.0 (http://www.apache.org/licenses/)
PDF.js
Apache License Version 2.0 (http://www.apache.org/licenses/)
Font Awesome
SIL OFL 1.1 (http://scripts.sil.org/OFL)
MIT License (http://opensource.org/licenses/mit-license.html)
CC BY 3.0 ( http://creativecommons.org/licenses/by/3.0/)
Powered by Worktribe © 2024
Advanced Search