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All Outputs (793)

Predicting effective pro-apoptotic antileukaemic drug combinations using cooperative dynamic BH3 profiling (2018)
Journal Article
Grundy, M., Seedhouse, C., Jones, T., Elmi, L., Hall, M., Graham, A., …Pallis, M. (2018). Predicting effective pro-apoptotic antileukaemic drug combinations using cooperative dynamic BH3 profiling. PLoS ONE, 13(1), Article e0190682. https://doi.org/10.1371/journal.pone.0190682

The BH3-only apoptosis agonists BAD and NOXA target BCL-2 and MCL-1 respectively and co-operate to induce apoptosis. On this basis, therapeutic drugs targeting BCL-2 and MCL-1 might have enhanced activity if used in combination. We identified anti-le... Read More about Predicting effective pro-apoptotic antileukaemic drug combinations using cooperative dynamic BH3 profiling.

MUSCLEMOTION: a versatile open software tool to quantify cardiomyocyte and cardiac muscle contraction in vitro and in vivo (2017)
Journal Article
Sala, L., van Meer, B., Tertoolen, L., Bakkers, J., Bellin, M., Davis, R., …Burton, F. (2018). MUSCLEMOTION: a versatile open software tool to quantify cardiomyocyte and cardiac muscle contraction in vitro and in vivo. Circulation Research, 122(3), e5. https://doi.org/10.1161/CIRCRESAHA.117.312067

Rationale: There are several methods to measure cardiomyocyte (CM) and muscle contraction but these require customized hardware, expensive apparatus and advanced informatics or can only be used in single experimental models. Consequently, data and te... Read More about MUSCLEMOTION: a versatile open software tool to quantify cardiomyocyte and cardiac muscle contraction in vitro and in vivo.

Model-based image analysis of a tethered Brownian fibre for shear stress sensing (2017)
Journal Article
Gallagher, M. T., Neal, C. V., Arkill, K. P., & Smith, D. J. (2017). Model-based image analysis of a tethered Brownian fibre for shear stress sensing. Interface, 14(137), Article 20170564. https://doi.org/10.1098/rsif.2017.0564

The measurement of fluid dynamic shear stress acting on a biologically relevant surface is a challenging problem, particularly in the complex environment of, for example, the vasculature. While an experimental method for the direct detection of wall... Read More about Model-based image analysis of a tethered Brownian fibre for shear stress sensing.

A molecular signature of dormancy in CD34+CD38- acute myeloid leukaemia cells (2017)
Journal Article
Al-Asadi, M. G., Brindle, G., Castellanos, M., May, S., Mills, K. I., Russell, N., …Pallis, M. (2017). A molecular signature of dormancy in CD34+CD38- acute myeloid leukaemia cells. Oncotarget, 8(67), 111405-111418. https://doi.org/10.18632/oncotarget.22808

Dormant leukaemia initiating cells in the bone marrow niche are a crucial therapeutic target for total eradication of acute myeloid leukaemia. To study this cellular subset we created and validated an in vitro model employing the cell line TF-1a, tre... Read More about A molecular signature of dormancy in CD34+CD38- acute myeloid leukaemia cells.

MYC regulation of Glutamine-Proline regulatory axis is key in Luminal B breast cancer (2017)
Journal Article
Craze, M. L., Cheung, H., Jewa, N., Coimbra, N. D., Soria, D., El-Ansari, R., Aleskandarany, M. A., Cheng, K. W., Diez-Rodriguez, M., Nolan, C. C., Ellis, I. O., Rakha, E., & Green, A. R. (in press). MYC regulation of Glutamine-Proline regulatory axis is key in Luminal B breast cancer. British Journal of Cancer, https://doi.org/10.1038/bjc.2017.387

Background: Altered cellular metabolism is a hallmark of cancer and some are reliant on Glutamine for sustained proliferation and survival. We hypothesise that the Glutamine-Proline regulatory axis has a key role in Breast cancer (BC) in the highly p... Read More about MYC regulation of Glutamine-Proline regulatory axis is key in Luminal B breast cancer.

Regulation of human feto-placental endothelial barrier integrity by vascular endothelial growth factors: competitive interplay between VEGF-A165a, VEGF-A165b, PIGF and VE-cadherin (2017)
Journal Article
Pang, V., Bates, D. O., & Leach, L. (2017). Regulation of human feto-placental endothelial barrier integrity by vascular endothelial growth factors: competitive interplay between VEGF-A165a, VEGF-A165b, PIGF and VE-cadherin. Clinical Science, 131(23), 2763-2775. https://doi.org/10.1042/CS20171252

The human placenta nourishes and protects the developing fetus whilst influencing maternal physiology for fetal advantage. It expresses several members of the VEGF family including the pro-angiogenic/pro-permeability VEGF-A165a isoform, the anti-angi... Read More about Regulation of human feto-placental endothelial barrier integrity by vascular endothelial growth factors: competitive interplay between VEGF-A165a, VEGF-A165b, PIGF and VE-cadherin.

Evaluation of CDK12 protein expression as a potential novel biomarker for DNA damage response–targeted therapies in breast cancer (2017)
Journal Article
Naidoo, K., Wai, P. T., Maguire, S. L., Daley, F., Haider, S., Kriplani, D., …Natrajan, R. (2018). Evaluation of CDK12 protein expression as a potential novel biomarker for DNA damage response–targeted therapies in breast cancer. Molecular Cancer Therapeutics, 17(1), 306-315. https://doi.org/10.1158/1535-7163.mct-17-0760

Disruption of Cyclin-Dependent Kinase 12 (CDK12) is known to lead to defects in DNA repair and sensitivity to platinum salts and PARP1/2 inhibitors. However, CDK12 has also been proposed as an oncogene in breast cancer. We therefore aimed to assess t... Read More about Evaluation of CDK12 protein expression as a potential novel biomarker for DNA damage response–targeted therapies in breast cancer.

Citrullinated α-enolase is an effective target for anti-cancer immunity (2017)
Journal Article
Cook, K., Daniels, I., Symonds, P., Pitt, T., Gijon, M., Xue, W., …Brentville, V. A. (2018). Citrullinated α-enolase is an effective target for anti-cancer immunity. OncoImmunology, 7(2), Article e1390642. https://doi.org/10.1080/2162402X.2017.1390642

Targeting post-translationally modified epitopes may provide a new strategy for generating tumor specific immune responses. Citrullination is the post-translational modification of arginine to citrulline catalyzed by peptidylarginine deaminase (PAD)... Read More about Citrullinated α-enolase is an effective target for anti-cancer immunity.

Comparative aspects of canine and human inflammatory breast cancer (2017)
Journal Article
Raposo, T., Arias-Pulido, H., Chaher, N., Fiering, S. N., Argyle, D. J., Prada, J., …Queiroga, F. L. (in press). Comparative aspects of canine and human inflammatory breast cancer. Seminars in Oncology, 44(4), https://doi.org/10.1053/j.seminoncol.2017.10.012

Inflammatory breast cancer (IBC) in humans is the most aggressive form of mammary gland cancer and shares clinical, pathologic, and molecular patterns of disease with canine inflammatory mammary carcinoma (CIMC). Despite the use of multimodal therape... Read More about Comparative aspects of canine and human inflammatory breast cancer.

In vitro tissue microarrays for quick and efficient spheroid characterisation (2017)
Journal Article
Ivanov, D. P., & Grabowska, A. M. (2018). In vitro tissue microarrays for quick and efficient spheroid characterisation. Slas Discovery, 23(2), https://doi.org/10.1177/2472555217740576

Three-dimensional in vitro microphysiological cultures, such as spheroids and organoids, promise increased patient relevance and therapeutic predictivity compared to reductionist cell monolayers. However, high-throughput characterisation techniques f... Read More about In vitro tissue microarrays for quick and efficient spheroid characterisation.

Racial and Ethnic Disparities in Cancer Survival: The Contribution of Tumor, Sociodemographic, Institutional, and Neighborhood Characteristics (2017)
Journal Article
Ellis, L., Canchola, A. J., Spiegel, D., Ladabaum, U., Haile, R., & Gomez, S. L. (2018). Racial and Ethnic Disparities in Cancer Survival: The Contribution of Tumor, Sociodemographic, Institutional, and Neighborhood Characteristics. Journal of Clinical Oncology, 36(1), 25-33. https://doi.org/10.1200/jco.2017.74.2049

Purpose
Racial/ethnic disparities in cancer survival in the United States are well documented, but the underlying causes are not well understood. We quantified the contribution of tumor, treatment, hospital, sociodemographic, and neighborhood factor... Read More about Racial and Ethnic Disparities in Cancer Survival: The Contribution of Tumor, Sociodemographic, Institutional, and Neighborhood Characteristics.

Drug-mediated shortening of action potentials in LQTS2 hiPSC-cardiomyocytes (2017)
Journal Article
Duncan, G., Firth, K. S., George, V., Hoang, M. D., Staniforth, A., Smith, G., & Denning, C. (2017). Drug-mediated shortening of action potentials in LQTS2 hiPSC-cardiomyocytes. Stem Cells and Development, 26(23), https://doi.org/10.1089/scd.2017.0172

Cardiomyocytes (CMs) derived from human induced pluripotent stem cells (hiPSCs) are now a well-established modality for modeling genetic disorders of the heart. This is especially so for long QT syndrome (LQTS), which is caused by perturbation of ion... Read More about Drug-mediated shortening of action potentials in LQTS2 hiPSC-cardiomyocytes.

Control of aggregation temperatures in mixed and blended cytocompatible thermoresponsive block co-polymer nanoparticles (2017)
Journal Article
Foralosso, R., Moir, L., Mastrotto, F., Sasso, L., Tchoryk, A., Selo, A., …Alexander, C. (2017). Control of aggregation temperatures in mixed and blended cytocompatible thermoresponsive block co-polymer nanoparticles. Soft Matter, 40(13), https://doi.org/10.1039/c7sm00920h

A small library of thermoresponsive amphiphilic copolymers based on polylactide-block-poly((2-(2-methoxyethoxy)ethyl methacrylate)-co-(oligoethylene glycol methacrylate)) (PLA-b-P(DEGMA)-co-(OEGMA)), was synthesised by copper-mediated controlled radi... Read More about Control of aggregation temperatures in mixed and blended cytocompatible thermoresponsive block co-polymer nanoparticles.

Sulfated galactans from the red seaweed Gracilaria fisheri exerts anti-migration effect on cholangiocarcinoma cells (2017)
Journal Article
Sae-Lao, T., Luplertlop, N., Janvilisri, T., Tohtong, R., Bates, D. O., & Wongprasert, K. (2017). Sulfated galactans from the red seaweed Gracilaria fisheri exerts anti-migration effect on cholangiocarcinoma cells. Phytomedicine, 36, https://doi.org/10.1016/j.phymed.2017.09.014

BACKGROUND: Seaweeds have a long history of use in Asian countries as functional foods, medicinal herbs, and the treatment of cancer. Polysaccharides from various seaweeds have shown anti-tumor activity. Cholangiocarcinoma (CCA), often with metastati... Read More about Sulfated galactans from the red seaweed Gracilaria fisheri exerts anti-migration effect on cholangiocarcinoma cells.

Cten promotes epithelial-mesenchymal transition through post-transcriptional stabilization of Snail (2017)
Journal Article
Thorpe, H., Asiri, A., Akhlaq, M., & Ilyas, M. (in press). Cten promotes epithelial-mesenchymal transition through post-transcriptional stabilization of Snail. Molecular Carcinogenesis, 56(12), https://doi.org/10.1002/mc.22704

Cten promotes cell migration however the knowledge of underlying signalling pathways is sparse. We have shown that Cten downregulates E-cadherin, a feature of epithelial to mesenchymal transition (EMT). This prompted us to investigate whether Cten fu... Read More about Cten promotes epithelial-mesenchymal transition through post-transcriptional stabilization of Snail.

Protein kinase CK2 inhibition suppresses neointima formation via a proline-rich homeodomain-dependent mechanism (2017)
Journal Article
Wadey, K., Brown, B., Sala-Newby, G., Jayaraman, P.-S., Gaston, K., & George, S. (2017). Protein kinase CK2 inhibition suppresses neointima formation via a proline-rich homeodomain-dependent mechanism. Vascular Pharmacology, 99, 34--44. https://doi.org/10.1016/j.vph.2017.09.004

Neointimal hyperplasia is a product of VSMC replication and consequent accumulation within the blood vessel wall. In this study, we determined whether inhibition of protein kinase CK2 and the resultant stabilisation of proline-rich homeodomain (PRH)... Read More about Protein kinase CK2 inhibition suppresses neointima formation via a proline-rich homeodomain-dependent mechanism.

Breast cancer induced nociceptor aberrant growth and collateral sensory axonal branching (2017)
Journal Article
Austin, M., Elliott, L., Nicolaou, N., Grabowska, A. M., & Hulse, R. P. (2017). Breast cancer induced nociceptor aberrant growth and collateral sensory axonal branching. Oncotarget, 8(44), https://doi.org/10.18632/oncotarget.20609

The tumour and neuron interaction has a significant impact upon disease progression and the patients quality of life. In breast cancer patients, it is known that there is an interaction between the tumour microenvironment and the sensory neurons to i... Read More about Breast cancer induced nociceptor aberrant growth and collateral sensory axonal branching.