Skip to main content

Research Repository

See what's under the surface

Advanced Search

In vitro tissue microarrays for quick and efficient spheroid characterisation

Ivanov, Delyan Pavlov; Grabowska, Anna M.

Authors

Delyan Pavlov Ivanov delyan.ivanov@nottingham.ac.uk

Anna M. Grabowska



Abstract

Three-dimensional in vitro microphysiological cultures, such as spheroids and organoids, promise increased patient relevance and therapeutic predictivity compared to reductionist cell monolayers. However, high-throughput characterisation techniques for 3D models are currently limited to simplistic live/dead assays. By sectioning and staining in vitro microtissues researchers can examine their structure, detect DNA, RNA and protein targets and visualise them at the level of single cells. The morphological examination and immunochemistry staining for in vitro cultures has historically been done in a laborious manner involving testing one set of cultures at a time. We have developed a technology to rapidly screen spheroid phenotype and protein expression by arranging 66 spheroids in a gel array for paraffin-embedding, sectioning and immunohistochemsitry. The process is quick, mostly automatable and uses 11 times less reagents compared to conventional techniques. Here we showcase the capabilities of the technique in an array made up of 11 different cell lines stained in conventional H&E staining, as well as immunohistochemistry staining for estrogen (ER), progesterone (PR) human epidermal growth factor receptors (Her-2) and TP53. This new methodology can be used in optimising stem cell-based models of disease and development, for tissue engineering, safety screening and for efficacy screens in cancer research.

Journal Article Type Article
Publication Date Feb 1, 2018
Journal Slas Discovery
Electronic ISSN 2472-5552
Publisher SAGE Publications
Peer Reviewed Peer Reviewed
Volume 23
Issue 2
APA6 Citation Ivanov, D. P., & Grabowska, A. M. (2018). In vitro tissue microarrays for quick and efficient spheroid characterisation. Slas Discovery, 23(2), https://doi.org/10.1177/2472555217740576
DOI https://doi.org/10.1177/2472555217740576
Keywords Single-cell analysis; Microphysiological systems; Three-dimensional cell culture, automation; Image analysis
Publisher URL http://journals.sagepub.com/doi/full/10.1177/2472555217740576
Copyright Statement Copyright information regarding this work can be found at the following address: http://creativecommons.org/licenses/by-nc/4.0

Files

2472555217740576.pdf (492 Kb)
PDF

Copyright Statement
Copyright information regarding this work can be found at the following address: http://creativecommons.org/licenses/by-nc/4.0





You might also like



Downloadable Citations

;