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Control of aggregation temperatures in mixed and blended cytocompatible thermoresponsive block co-polymer nanoparticles

Foralosso, Ruggero; Moir, Lee; Mastrotto, Francesca; Sasso, Luana; Tchoryk, Aleksandra; Selo, Amjad; Grabowska, Anna M.; Ashford, Marianne B.; Aylott, Jonathan W.; Gellert, Paul R.; Spain, Sebastian G.; Alexander, Cameron

Authors

Ruggero Foralosso Ruggero.Foralosso@nottingham.ac.uk

Lee Moir

Francesca Mastrotto francesca.mastrotto@nottingham.ac.uk

Luana Sasso

Aleksandra Tchoryk Aleksandra.Tchoryk@nottingham.ac.uk

Amjad Selo Amjad.Abouselo@nottingham.ac.uk

Anna M. Grabowska

Marianne B. Ashford

Jonathan W. Aylott

Paul R. Gellert

Sebastian G. Spain sebastian.spain@nottingham.ac.uk

Cameron Alexander



Abstract

A small library of thermoresponsive amphiphilic copolymers based on polylactide-block-poly((2-(2-methoxyethoxy)ethyl methacrylate)-co-(oligoethylene glycol methacrylate)) (PLA-b-P(DEGMA)-co-(OEGMA)), was synthesised by copper-mediated controlled radical polymerisation (CRP) with increasing ratios of OEGMA:DEGMA. These polymers were combined in two ways to form nanoparticles with controllable thermal transition temperatures as measured by particle aggregation. The first technique involved the blending of two (PLA-b-P(DEGMA)-co-(OEGMA)) polymers together prior to assembling NPs. The second method involved mixing pre-formed nanoparticles of single (PLA-b-P(DEGMA)-co-(OEGMA)) polymers. The observed critical aggregation temperature Tt did not change in a linear relationship with the ratios of each copolymer either in the nanoparticles blended from different copolymers or in the mitures of pre-formed nanoparticles. However, where co-polymer mixtures were based on (OEG)9MA ratios within 5-10 mole% , a linear relationship between (OEG)9MA composition in the blends and Tt was obtained. The data suggest that OEGMA-based copolymers are tunable over a wide temperature range given suitable co-monomer content in the linear polymers or nanoparticles. Moreover, the thermal transitions of the nanoparticles were reversible and repeatable, with the cloud point curves being essentially invariant across at least three heating and cooling cycles, and a selected nanoparticle formulation was found to be readily endocytosed in representative cancer cells and fibroblasts.

Journal Article Type Article
Publication Date Oct 2, 2017
Journal Soft Matter
Print ISSN 1744-683X
Electronic ISSN 1744-6848
Publisher Royal Society of Chemistry
Peer Reviewed Peer Reviewed
Volume 40
Issue 13
APA6 Citation Foralosso, R., Moir, L., Mastrotto, F., Sasso, L., Tchoryk, A., Selo, A., …Alexander, C. (2017). Control of aggregation temperatures in mixed and blended cytocompatible thermoresponsive block co-polymer nanoparticles. Soft Matter, 40(13), https://doi.org/10.1039/c7sm00920h
DOI https://doi.org/10.1039/c7sm00920h
Publisher URL http://pubs.rsc.org/en/Content/ArticleLanding/2017/SM/C7SM00920H#!divAbstract
Copyright Statement Copyright information regarding this work can be found at the following address: http://creativecommons.org/licenses/by/4.0

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Copyright Statement
Copyright information regarding this work can be found at the following address: http://creativecommons.org/licenses/by/4.0





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