MUSCLEMOTION: a versatile open software tool to quantify cardiomyocyte and cardiac muscle contraction in vitro and in vivo

Sala, L.; van Meer, B.J.; Tertoolen, L.G.J.; Bakkers, J.; Bellin, M.; Davis, R.P.; Denning, Chris; Dieben, M.A.E.; Eschenhagen, T.; Giacomelli, E.; Grandela, C.; Hansen, A.; Holman, E.R.; Jongbloed, M.R.M.; Kamel, S.M.; Koopman, C.D.; Lachaud, Q.; Mannhardt, I.; Mol, M.P.H.; Mosqueira, D.; Orlova, V.V.; Passier, R.; Ribeiro, M.C.; Saleem, U.; Smith, G.L.; Mummery, C.L.; Burton, F.L.

Abstract

Rationale: There are several methods to measure cardiomyocyte (CM) and muscle contraction but these require customized hardware, expensive apparatus and advanced informatics or can only be used in single experimental models. Consequently, data and techniques have been difficult to reproduce across models and laboratories, analysis is time consuming and only specialist researchers can quantify data.
Objective: Here we describe and validate an automated, open source software tool (MUSCLEMOTION) adaptable for use with standard laboratory- and clinical imaging equipment that enables quantitative analysis of normal cardiac contraction, disease phenotypes and pharmacological responses.
Methods and Results: MUSCLEMOTION allowed rapid and easy measurement of contractility from high-speed movies in: (i) 1-dimensional in vitro models such as isolated adult and human pluripotent stem cell-derived CMs (hPSC-CMs); (ii) 2-dimensional in vitro models, such as beating CM monolayers or small clusters of hPSC-CMs; (iii) 3-dimensional multicellular in vitro or in vivo contractile tissues such as cardiac "organoids", engineered heart tissues (EHT), zebrafish- and human hearts. MUSCLEMOTION was effective under different recording conditions (bright field microscopy with simultaneous patch clamp recording, phase contrast microscopy and traction force microscopy). Outcomes were virtually identical to the current gold standards for contraction measurement such as optical flow, pole deflection, edge-detection systems or manual analyses. Finally, we used the algorithm to quantify contraction in in vitro and in vivo arrhythmia models and to measure pharmacological responses.
Conclusions: Using a single open source method for processing video recordings, we obtained reliable pharmacological data and measures of cardiac disease phenotype in experimental cell-, animal- and human models

Citation

Sala, L., van Meer, B., Tertoolen, L., Bakkers, J., Bellin, M., Davis, R., Denning, C., Dieben, M., Eschenhagen, T., Giacomelli, E., Grandela, C., Hansen, A., Holman, E., Jongbloed, M., Kamel, S., Koopman, C., Lachaud, Q., Mannhardt, I., Mol, M., Mosqueira, D., …Burton, F. (2018). MUSCLEMOTION: a versatile open software tool to quantify cardiomyocyte and cardiac muscle contraction in vitro and in vivo. Circulation Research, 122(3), e5. https://doi.org/10.1161/CIRCRESAHA.117.312067

• Journal Article Type: Article
• Acceptance Date: Dec 23, 2017
• Online Publication Date: Dec 27, 2017
• Publication Date: Feb 2, 2018
• Deposit Date: Jan 9, 2018
• Publicly Available Date: Mar 14, 2019
• Journal: Circulation Research
• Print ISSN: 0009-7330
• Electronic ISSN: 1524-4571
• Publisher: American Heart Association
• Peer Reviewed: Peer Reviewed
• Volume: 122
• Issue: 3
• Pages: e5
• Series Title: e16
• DOI: https://doi.org/10.1161/CIRCRESAHA.117.312067
• Keywords: Pluripotent stem Cell-derived cardiomyocytes, contraction, basic science; arrhythmia, software tools
• Public URL: https://nottingham-repository.worktribe.com/output/901883
• Publisher URL: http://circres.ahajournals.org/content/early/2017/12/26/CIRCRESAHA.117.312067
• Contract Date: Jan 9, 2018