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CRISPR-Cas adaptation in Escherichia coli (2023)
Journal Article
Mitic, D., Bolt, E. L., & Ivancic-Bace, I. (2023). CRISPR-Cas adaptation in Escherichia coli. Bioscience Reports, 43(3), Article BSR20221198. https://doi.org/10.1042/BSR20221198

Prokaryotes use the adaptive immunity mediated via the Clustered Regularly Interspaced Short Palindromic Repeats and CRISPR associated (CRISPR-Cas) system for protection against invading elements such as phages and plasmids. The immunity is achieved... Read More about CRISPR-Cas adaptation in Escherichia coli.

A Sublethal Concentration of Sulfoxaflor Has Minimal Impact on Buff-Tailed Bumblebee (Bombus terrestris) Locomotor Behaviour under Aversive Conditioning (2023)
Journal Article
James, L., Reynolds, A. M., Mellor, I. R., & Davies, T. G. E. (2023). A Sublethal Concentration of Sulfoxaflor Has Minimal Impact on Buff-Tailed Bumblebee (Bombus terrestris) Locomotor Behaviour under Aversive Conditioning. Toxics, 11(3), Article 279. https://doi.org/10.3390/toxics11030279

Pesticide exposure has been cited as a key threat to insect pollinators. Notably, a diverse range of potential sublethal effects have been reported in bee species, with a particular focus on effects due to exposure to neonicotinoid insecticides. Here... Read More about A Sublethal Concentration of Sulfoxaflor Has Minimal Impact on Buff-Tailed Bumblebee (Bombus terrestris) Locomotor Behaviour under Aversive Conditioning.

m6A mRNA methylation in human brain is disrupted in Lewy body disorders (2023)
Journal Article
Martinez De La Cruz, B., Gell, C., Markus, R., Macdonald, I., Fray, R., & Knight, H. M. (2023). m6A mRNA methylation in human brain is disrupted in Lewy body disorders. Neuropathology and Applied Neurobiology, 49(1), Article e12885. https://doi.org/10.1111/nan.12885

N6-methyladenosine modification of RNA (m6A) regulates translational control which may influence neuronal dysfunction underlying neurodegenerative diseases. Using microscopy and a machine learning approach, we performed cellular profiling of m6A-RNA... Read More about m6A mRNA methylation in human brain is disrupted in Lewy body disorders.

A proposed framework to evaluate the quality and reliability of targeted metabolomics assays from the UK Consortium on Metabolic Phenotyping (MAP/UK) (2023)
Journal Article
Sarmad, S., Viant, M. R., Dunn, W. B., Goodacre, R., Wilson, I. D., Chappell, K. E., …UK Consortium on Metabolic Phenotyping (MAP/UK). (2023). A proposed framework to evaluate the quality and reliability of targeted metabolomics assays from the UK Consortium on Metabolic Phenotyping (MAP/UK). Nature Protocols, 18(4), 1017-1027. https://doi.org/10.1038/s41596-022-00801-8

Targeted metabolite assays that measure tens or hundreds of pre-selected metabolites, typically using liquid chromatography–mass spectrometry, are increasingly being developed and applied to metabolic phenotyping studies. These are used both as stand... Read More about A proposed framework to evaluate the quality and reliability of targeted metabolomics assays from the UK Consortium on Metabolic Phenotyping (MAP/UK).

Variable disruption of epithelial monolayers by Neisseria meningitidis carriage isolates of the hypervirulent MenW cc11 and MenY cc23 lineages (2023)
Journal Article
Dave, N., Albiheyri, R. S., Wanford, J. J., Green, L. R., Oldfield, N. J., Turner, D. P., …Bayliss, C. D. (2023). Variable disruption of epithelial monolayers by Neisseria meningitidis carriage isolates of the hypervirulent MenW cc11 and MenY cc23 lineages. Microbiology, 169(2), https://doi.org/10.1099/mic.0.001305

Colonization of mucosal tissues by Neisseria meningitidis requires adhesion mediated by the type IV pilus and multiple outer-membrane proteins. Penetration of the mucosa and invasion of epithelial cells are thought to contribute to host persistence a... Read More about Variable disruption of epithelial monolayers by Neisseria meningitidis carriage isolates of the hypervirulent MenW cc11 and MenY cc23 lineages.

The importance of m6A topology in chicken embryo mRNA: a precise mapping of m6A at the conserved chicken β-actin zipcode (2023)
Journal Article
Baron, F., Zhang, M., Archer, N., Bellows, E., Knight, H. M., Welham, S., …Bodi, Z. (2023). The importance of m6A topology in chicken embryo mRNA: a precise mapping of m6A at the conserved chicken β-actin zipcode. RNA, 29(6), 777-789. https://doi.org/10.1261/rna.079615.123

N6-methyladenosine (m6A) in mRNA regulates almost every stage in the mRNA life cycle, and the development of methodologies for the high-throughput detection of methylated sites in mRNA using m6A-specific methylated RNA immunoprecipitation with next-g... Read More about The importance of m6A topology in chicken embryo mRNA: a precise mapping of m6A at the conserved chicken β-actin zipcode.

The impact of physical inactivity on glucose homeostasis when diet is adjusted to maintain energy balance in healthy, young males (2023)
Journal Article
Trim, W. V., Walhin, J. P., Koumanov, F., Turner, J. E., Shur, N. F., Simpson, E. J., …Thompson, D. (2023). The impact of physical inactivity on glucose homeostasis when diet is adjusted to maintain energy balance in healthy, young males. Clinical Nutrition, 42(4), 532-540. https://doi.org/10.1016/j.clnu.2023.02.006

Background & aims: It is unclear if dietary adjustments to maintain energy balance during reduced physical activity can offset inactivity-induced reductions in insulin sensitivity and glucose disposal to produce normal daily glucose concentrations an... Read More about The impact of physical inactivity on glucose homeostasis when diet is adjusted to maintain energy balance in healthy, young males.

Design of Quorum Sensing Inhibitor–Polymer Conjugates to Penetrate Pseudomonas aeruginosa Biofilms (2023)
Journal Article
Soukarieh, F., Gurnani, P., Romero, M., Halliday, N., Stocks, M., Alexander, C., & Cámara, M. (2023). Design of Quorum Sensing Inhibitor–Polymer Conjugates to Penetrate Pseudomonas aeruginosa Biofilms. ACS Macro Letters, 12(3), 314-319. https://doi.org/10.1021/acsmacrolett.2c00699

Antimicrobial resistance (AMR) is a global threat to public health with a forecast of a negative financial impact of one trillion dollars per annum, hence novel therapeutics are urgently needed. The resistance of many bacteria against current drugs i... Read More about Design of Quorum Sensing Inhibitor–Polymer Conjugates to Penetrate Pseudomonas aeruginosa Biofilms.

Exploring the Relationship between Polymer Surface Chemistry and Bacterial Attachment Using ToF-SIMS and Self-Organizing maps (2023)
Journal Article
Wong, S. Y., Hook, A. L., Gardner, W., Chang, C., Mei, Y., Davies, M. C., …Pigram, P. J. (2023). Exploring the Relationship between Polymer Surface Chemistry and Bacterial Attachment Using ToF-SIMS and Self-Organizing maps. Advanced Materials Interfaces, 10(9), Article 2202334. https://doi.org/10.1002/admi.202202334

Biofilm formation is a major cause of hospital-acquired infections. Research into biofilm-resistant materials is therefore critical to reduce the frequency of these events. Polymer microarrays offer a high-throughput approach to enable the efficient... Read More about Exploring the Relationship between Polymer Surface Chemistry and Bacterial Attachment Using ToF-SIMS and Self-Organizing maps.

Developing a genetic engineering method for Acetobacterium wieringae to expand one-carbon valorization pathways (2023)
Journal Article
Moreira, J. P., Heap, J. T., Alves, J. I., & Domingues, L. (2023). Developing a genetic engineering method for Acetobacterium wieringae to expand one-carbon valorization pathways. Biotechnology for Biofuels and Bioproducts, 16(1), Article 24. https://doi.org/10.1186/s13068-023-02259-6

Background: Developing new bioprocesses to produce chemicals and fuels with reduced production costs will greatly facilitate the replacement of fossil-based raw materials. In most fermentation bioprocesses, the feedstock usually represents the highes... Read More about Developing a genetic engineering method for Acetobacterium wieringae to expand one-carbon valorization pathways.

Cysteine-Selective Modification of Peptides and Proteins via Desulfurative C−C Bond Formation (2023)
Journal Article
Griffiths, R. C., Smith, F. R., Li, D., Wyatt, J., Rogers, D. M., Long, J. E., …Mitchell, N. (2023). Cysteine-Selective Modification of Peptides and Proteins via Desulfurative C−C Bond Formation. Chemistry - A European Journal, 29(16), Article e202202503. https://doi.org/10.1002/chem.202202503

The site-selective modification of peptides and proteins facilitates the preparation of targeted therapeutic agents and tools to interrogate biochemical pathways. Among the numerous bioconjugation techniques developed to install groups of interest, t... Read More about Cysteine-Selective Modification of Peptides and Proteins via Desulfurative C−C Bond Formation.

Individual level microbial communities in the digestive system of the freshwater isopod Asellus aquaticus: Complex, robust and prospective (2023)
Journal Article
Liao, A., Hartikainen, H., & Buser, C. C. (2023). Individual level microbial communities in the digestive system of the freshwater isopod Asellus aquaticus: Complex, robust and prospective. Environmental Microbiology Reports, 15(3), 188-196. https://doi.org/10.1111/1758-2229.13142

The freshwater isopod Asellus aquaticus is an important decomposer of leaf detritus, and its diverse gut microbiome has been depicted as key contributors in lignocellulose degradation as of terrestrial isopods. However, it is not clear whether the in... Read More about Individual level microbial communities in the digestive system of the freshwater isopod Asellus aquaticus: Complex, robust and prospective.

Drug Resistance in Medulloblastoma Is Driven by YB-1, ABCB1 and a Seven-Gene Drug Signature (2023)
Journal Article
Taylor, L., Wade, P. K., Johnson, J. E. C., Aldighieri, M., Morlando, S., Di Leva, G., …Coyle, B. (2023). Drug Resistance in Medulloblastoma Is Driven by YB-1, ABCB1 and a Seven-Gene Drug Signature. Cancers, 15(4), Article 1086. https://doi.org/10.3390/cancers15041086

Therapy resistance represents an unmet challenge in the treatment of medulloblastoma. Accordingly, the identification of targets that mark drug-resistant cell populations, or drive the proliferation of resistant cells, may improve treatment strategie... Read More about Drug Resistance in Medulloblastoma Is Driven by YB-1, ABCB1 and a Seven-Gene Drug Signature.

A comparative, randomised MRI study of the physiological and appetitive responses to gelling (alginate) and non-gelling nasogastric tube feeds in healthy men (2023)
Journal Article
Aliyu, A. I., Nixon, A., Hoad, C., Marciani, L., Corsetti, M., Aithal, G. P., …Taylor, M. A. (2023). A comparative, randomised MRI study of the physiological and appetitive responses to gelling (alginate) and non-gelling nasogastric tube feeds in healthy men. British Journal of Nutrition, 130(8), 1316-1328. https://doi.org/10.1017/S0007114523000302

AbstractInclusion in nasogastric tube feeds (NGTF) of acid-sensitive, seaweed-derived alginate, expected to form a reversible gel in the stomach, may create a more normal intragastric state and modified gastrointestinal responses. This may ameliorate... Read More about A comparative, randomised MRI study of the physiological and appetitive responses to gelling (alginate) and non-gelling nasogastric tube feeds in healthy men.

Arbovirus circulation, epidemiology and spatiotemporal distribution in Uganda (2023)
Journal Article
Byaruhanga, T., Kayiwa, J. T., Nankya, A. M., Ataliba, I. J., McClure, C. P., Ball, J. K., & Lutwama, J. J. (2023). Arbovirus circulation, epidemiology and spatiotemporal distribution in Uganda. IJID Regions, 6, 171-176. https://doi.org/10.1016/j.ijregi.2023.01.013

Background: Arboviruses are endemic in Uganda; however, little is known about their epidemiology, seasonality and spatiotemporal distribution. Our study sought to provide information on arbovirus outbreaks from acute clinical presentations. Methods:... Read More about Arbovirus circulation, epidemiology and spatiotemporal distribution in Uganda.

Interaction of human HelQ with DNA polymerase delta halts DNA synthesis and stimulates DNA single-strand annealing (2023)
Journal Article
He, L., Lever, R., Cubbon, A., Tehseen, M., Jenkins, T., Nottingham, A. O., …Bolt, E. L. (2023). Interaction of human HelQ with DNA polymerase delta halts DNA synthesis and stimulates DNA single-strand annealing. Nucleic Acids Research, 51(4), 1740-1749. https://doi.org/10.1093/nar/gkad032

DNA strand breaks are repaired by DNA synthesis from an exposed DNA end paired with a homologous DNA template. DNA polymerase delta (Pol δ) catalyses DNA synthesis in multiple eukaryotic DNA break repair pathways but triggers genome instability unles... Read More about Interaction of human HelQ with DNA polymerase delta halts DNA synthesis and stimulates DNA single-strand annealing.

Evaluating the potential of natural product combinations with sorbic acid for improving preservative action against food-spoilage yeasts (2023)
Journal Article
Harvey, H. J., Hendry, A. C., Archer, D. B., & Avery, S. V. (2023). Evaluating the potential of natural product combinations with sorbic acid for improving preservative action against food-spoilage yeasts. Fungal Biology, 127(7-8), 1218-1223. https://doi.org/10.1016/j.funbio.2023.01.004

Fungal control methods commonly involve the use of antifungals or preservatives, which can raise concerns about broader effects of these stressors on non-target organisms, spread of resistance and regulatory hurdles. Consequently, control methods ena... Read More about Evaluating the potential of natural product combinations with sorbic acid for improving preservative action against food-spoilage yeasts.

Fourth Report on Chicken Genes and Chromosomes 2022 (2023)
Journal Article
Smith, J., Alfieri, J. M., Anthony, N., Arensburger, P., Athrey, G. N., Balacco, J., …Zhou, H. (2023). Fourth Report on Chicken Genes and Chromosomes 2022. Cytogenetic and Genome Research, 162(8-9), 405–528. https://doi.org/10.1159/000529376

The chicken continues to hold its position as a leading model organism within many areas of research, as well as being a major source of protein for human consumption. The First Report on Chicken Genes and Chromosomes [Schmid et al., 2000], which was... Read More about Fourth Report on Chicken Genes and Chromosomes 2022.

Delivery of biannual ultrasound surveillance for individuals with cirrhosis and cured hepatitis C in the UK (2023)
Journal Article
Hamill, V., Gelson, W., MacDonald, D., Richardson, P., Ryder, S. D., Aldersley, M., …Innes, H. (2023). Delivery of biannual ultrasound surveillance for individuals with cirrhosis and cured hepatitis C in the UK. Liver International, 43(4), 917-927. https://doi.org/10.1111/liv.15528

Background: Previous studies show the uptake of biannual ultrasound (US) surveillance in patients with cirrhosis is suboptimal. Here, our goal was to understand in broader terms how surveillance is being delivered to cirrhosis patients with cured hep... Read More about Delivery of biannual ultrasound surveillance for individuals with cirrhosis and cured hepatitis C in the UK.