All Outputs (709)

Evaluation of codA, tms2, and ABRIN-A as negative selectable markers in transgenic tobacco and rice (2014)
Journal Article
Majhi, B. B., Bhosale, R., Jawkar, S., & Veluthambi, K. (2014). Evaluation of codA, tms2, and ABRIN-A as negative selectable markers in transgenic tobacco and rice. In Vitro Cellular and Developmental Biology - Plant, 50(5), 541-551. https://doi.org/10.1007/s11627-014-9625-1

© 2014, The Society for In Vitro Biology. The codA and tms2 genes are used as efficient conditional negative selectable markers (NSMs) in several dicotyledonous plants. We evaluated both genes under control of the CaMV 35S promoter for their effectiv... Read More about Evaluation of codA, tms2, and ABRIN-A as negative selectable markers in transgenic tobacco and rice.

Engineering a reagentless biosensor for single-stranded DNA to measure real-time helicase activity in Bacillus (2014)
Journal Article
Green, M., Gilhooly, N. S., Abedeen, S., Scott, D. J., Dillingham, M. S., & Soultanas, P. (2014). Engineering a reagentless biosensor for single-stranded DNA to measure real-time helicase activity in Bacillus. Biosensors and Bioelectronics, 61, 579-586. https://doi.org/10.1016/j.bios.2014.06.011

Single-stranded DNA-binding protein (SSB) is a well characterized ubiquitous and essential bacterial protein involved in almost all aspects of DNA metabolism. Using the Bacillus subtilis SSB we have generated a reagentless SSB biosensor that can be u... Read More about Engineering a reagentless biosensor for single-stranded DNA to measure real-time helicase activity in Bacillus.

Factors affecting diet, habitat selection and breeding success of the African Crowned Eagle Stephanoaetus coronatus in a fragmented landscape (2014)
Journal Article
Swatridge, C. J., Monadjem, A., Steyn, D. J., Batchelor, G. R., & Hardy, I. C. (in press). Factors affecting diet, habitat selection and breeding success of the African Crowned Eagle Stephanoaetus coronatus in a fragmented landscape. Ostrich: Journal of African Ornithology, 85(1), https://doi.org/10.2989/00306525.2014.896832

This study aimed to identify variables that affect habitat selection and nesting success of the African Crowned Eagle Stephanoaetus coronatus, the largest forest raptor, in north-eastern South Africa. A preference for nesting in the Northern Mistbelt... Read More about Factors affecting diet, habitat selection and breeding success of the African Crowned Eagle Stephanoaetus coronatus in a fragmented landscape.

Limiting antenatal weight gain improves maternal health outcomes in severely obese pregnant women: findings of a pragmatic evaluation of a midwife-led intervention (2014)
Journal Article
McGiveron, A., Foster, S., Pearce, J., Taylor, M. A., McMullen, S., & Langley-Evans, S. C. (2014). Limiting antenatal weight gain improves maternal health outcomes in severely obese pregnant women: findings of a pragmatic evaluation of a midwife-led intervention. Journal of Human Nutrition and Dietetics, 28(S1), https://doi.org/10.1111/jhn.12240

Background: Antenatal obesity in pregnancy is associated with complications of pregnancy and poor obstetric outcomes. Although most guidance on pregnancy weight is focused on the pre-pregnancy period, pregnancy is widely viewed as a period where wome... Read More about Limiting antenatal weight gain improves maternal health outcomes in severely obese pregnant women: findings of a pragmatic evaluation of a midwife-led intervention.

Responsible innovation across borders: tensions, paradoxes and possibilities (2014)
Journal Article
Macnaghten, P., Owen, R., Stilgoe, J., Wynne, B., Azevedo, A., de Campos, A., …Velho, L. (2014). Responsible innovation across borders: tensions, paradoxes and possibilities. Journal of Responsible Innovation, 1(2), 191-199. https://doi.org/10.1080/23299460.2014.922249

In March 2014 a group of early career researchers and academics from São Paulo state and from the UK met at the University of Campinas to participate in a workshop on ‘Responsible Innovation and the Governance of Socially Controversial Technologies’.... Read More about Responsible innovation across borders: tensions, paradoxes and possibilities.

Higher aggression towards closer relatives by soldier larvae in a polyembryonic wasp (2014)
Journal Article
Dunn, J., Dunn, D. W., Strand, M. R., & Hardy, I. C. (2014). Higher aggression towards closer relatives by soldier larvae in a polyembryonic wasp. Biology Letters, 10(5), Article 20140229. https://doi.org/10.1098/rsbl.2014.0229

In the polyembryonic wasp Copidosoma floridanum, females commonly lay one male and one female egg in a lepidopteran host. Both sexes proliferate clonally within the growing host larva. Distinct larval castes develop from each wasp egg, the majority b... Read More about Higher aggression towards closer relatives by soldier larvae in a polyembryonic wasp.

Structure and Catalytic Regulatory Function of Ubiquitin Specific Protease 11 N-Terminal and Ubiquitin-like Domains (2014)
Journal Article
Harper, S., Gratton, H. E., Cornaciu, I., Oberer, M., Scott, D. J., Emsley, J., & Dreveny, I. (2014). Structure and Catalytic Regulatory Function of Ubiquitin Specific Protease 11 N-Terminal and Ubiquitin-like Domains. Biochemistry, 53(18), 2966-2978. https://doi.org/10.1021/bi500116x

The ubiquitin specific protease 11 (USP11) is implicated in DNA repair, viral RNA replication, and TGFβ signaling. We report the first characterization of the USP11 domain architecture and its role in regulating the enzymatic activity. USP11 consists... Read More about Structure and Catalytic Regulatory Function of Ubiquitin Specific Protease 11 N-Terminal and Ubiquitin-like Domains.

Influence of the in vivo method and basal dietary ingredients employed in the determination of the amino acid digestibility of wheat distillers dried grains with solubles in broilers (2014)
Journal Article
O'Neill, H. M., White, G. A., Li, D., Bedford, M. R., Htoo, J. K., & Wiseman, J. (2014). Influence of the in vivo method and basal dietary ingredients employed in the determination of the amino acid digestibility of wheat distillers dried grains with solubles in broilers. Poultry Science, 93(5), 1178-1185. https://doi.org/10.3382/ps.2013-03578

As distillers dried grains with solubles (DDGS) become increasingly available, it is important to determine their nutritional value for precise feed formulation. The accurate determination of digestibility is crucial, and it is known that the methods... Read More about Influence of the in vivo method and basal dietary ingredients employed in the determination of the amino acid digestibility of wheat distillers dried grains with solubles in broilers.

Anomalous Small Angle X-Ray Scattering Simulations: Proof of Concept for Distance Measurements for Nanoparticle-Labelled Biomacromolecules in Solution (2014)
Journal Article
Pinfield, V. J., & Scott, D. J. (2014). Anomalous Small Angle X-Ray Scattering Simulations: Proof of Concept for Distance Measurements for Nanoparticle-Labelled Biomacromolecules in Solution. PLoS ONE, 9(4), Article e95664. https://doi.org/10.1371/journal.pone.0095664

Anomalous small angle X-ray scattering can in principle be used to determine distances between metal label species on biological molecules. Previous experimental studies in the past were unable to distinguish the label-label scattering contribution f... Read More about Anomalous Small Angle X-Ray Scattering Simulations: Proof of Concept for Distance Measurements for Nanoparticle-Labelled Biomacromolecules in Solution.

On hydrodynamic methods for the analysis of the sizes and shapes of polysaccharides in dilute solution: a short review (2014)
Journal Article
Morris, G. A., Adams, G. G., & Harding, S. E. (2014). On hydrodynamic methods for the analysis of the sizes and shapes of polysaccharides in dilute solution: a short review. Food Hydrocolloids, 42, https://doi.org/10.1016/j.foodhyd.2014.04.014

Background: Polysaccharides and their derivatives are increasingly being used by the food, cosmetic and pharmaceutical industries: physical properties like size and conformation are important contributors to their performance. Discussion: Here the u... Read More about On hydrodynamic methods for the analysis of the sizes and shapes of polysaccharides in dilute solution: a short review.

Comparative heterogeneity, molecular weights and viscosities of xanthans of different pyruvate and acetate content (2014)
Journal Article
Erten, T., Adams, G. G., Foster, T. J., & Harding, S. E. (2014). Comparative heterogeneity, molecular weights and viscosities of xanthans of different pyruvate and acetate content. Food Hydrocolloids, 42, https://doi.org/10.1016/j.foodhyd.2014.04.012

Introduction: The bacterial exopolysaccharide xanthan is a well-known hydrocolloid, with a high viscosity deriving from its large molecular weight and volume. Materials and methods: Four different xanthans deriving from Xanthomanas campestris -two w... Read More about Comparative heterogeneity, molecular weights and viscosities of xanthans of different pyruvate and acetate content.

Ras GTPase-like protein MglA, a controller of bacterial social-motility in Myxobacteria, has evolved to control bacterial predation by Bdellovibrio (2014)
Journal Article
Milner, D. S., Till, R., Cadby, I., Lovering, A. L., Basford, S. M., Saxon, E. B., …Sockett, R. E. (2014). Ras GTPase-like protein MglA, a controller of bacterial social-motility in Myxobacteria, has evolved to control bacterial predation by Bdellovibrio. PLoS Genetics, 10(4), Article e1004253. https://doi.org/10.1371/journal.pgen.1004253

Bdellovibrio bacteriovorus invade Gram-negative bacteria in a predatory process requiring Type IV pili (T4P) at a single invasive pole, and also glide on surfaces to locate prey. Ras-like G-protein MglA, working with MglB and RomR in the deltaproteob... Read More about Ras GTPase-like protein MglA, a controller of bacterial social-motility in Myxobacteria, has evolved to control bacterial predation by Bdellovibrio.

New Strigolactone Analogs as Plant Hormones with Low Activities in the Rhizosphere (2014)
Journal Article
Boyer, F. D., de Saint Germain, A., Pouvreau, J.-B., Clavé, G., Pillot, J.-P., Roux, A., …Rameau, C. (2014). New Strigolactone Analogs as Plant Hormones with Low Activities in the Rhizosphere. Molecular Plant, 7(4), 675-690. https://doi.org/10.1093/mp/sst163

Strigolactones (SLs) are known not only as plant hormones, but also as rhizosphere signals for establishing symbiotic and parasitic interactions. The design of new specific SL analogs is a challenging goal in understanding the basic plant biology and... Read More about New Strigolactone Analogs as Plant Hormones with Low Activities in the Rhizosphere.

A barley PHD finger transcription factor that confers male sterility by affecting tapetal development (2014)
Journal Article
Fernandez Gomez, J. F., & Wilson, Z. A. (2014). A barley PHD finger transcription factor that confers male sterility by affecting tapetal development. Plant Biotechnology Journal, 12(6), https://doi.org/10.1111/pbi.12181

Controlling pollen development is of major commercial importance in generating hybrid crops and selective breeding, but characterized genes for male sterility in crops are rare, with no current examples in barley. However, translation of knowledge fr... Read More about A barley PHD finger transcription factor that confers male sterility by affecting tapetal development.

Activation of 2′ 5′-oligoadenylate synthetase by stem loops at the 5′-end of the West Nile virus genome (2014)
Journal Article
Deo, S., Patel, T. R., Dzananovic, E., Booy, E. P., Zeid, K., McEleney, K., …McKenna, S. A. (2014). Activation of 2′ 5′-oligoadenylate synthetase by stem loops at the 5′-end of the West Nile virus genome. PLoS ONE, 9(3), Article e92545. https://doi.org/10.1371/journal.pone.0092545

West Nile virus (WNV) has a positive sense RNA genome with conserved structural elements in the 5′ and 3′ -untranslated regions required for polyprotein production. Antiviral immunity to WNV is partially mediated through the production of a cluster o... Read More about Activation of 2′ 5′-oligoadenylate synthetase by stem loops at the 5′-end of the West Nile virus genome.

Systems Analysis of Auxin Transport in the Arabidopsis Root Apex (2014)
Journal Article
Band, L. R., Wells, D. M., Fozard, J. A., Ghetiu, T., French, A. P., Pound, M. P., …Bennett, M. J. (2014). Systems Analysis of Auxin Transport in the Arabidopsis Root Apex. Plant Cell, 26(3), 862-875. https://doi.org/10.1105/tpc.113.119495

Auxin is a key regulator of plant growth and development. Within the root tip, auxin distribution plays a crucial role specifying developmental zones and coordinating tropic responses. Determining how the organ-scale auxin pattern is regulated at the... Read More about Systems Analysis of Auxin Transport in the Arabidopsis Root Apex.

Effects of assisted reproductive technologies on human sex ratio at birth (2014)
Journal Article
Maalouf, W. E., Mincheva, M. N., Campbell, B. K., & Hardy, I. C. (2014). Effects of assisted reproductive technologies on human sex ratio at birth. Fertility and Sterility, 101(5), https://doi.org/10.1016/j.fertnstert.2014.01.041

Objective To investigate the effect of assisted reproductive technology (ART) treatments on the sex ratio of babies born. Design Assessment of direct effects of assisted conception through retrospective data analysis on the progeny sex ratio o... Read More about Effects of assisted reproductive technologies on human sex ratio at birth.

One hundred years of parasitoids (2014)
Journal Article
Kapranas, A., & Hardy, I. C. (2014). One hundred years of parasitoids. Biocontrol News and Information, 35(1), Article 1N-4N

Development of microsatellite markers and detection of genetic variation between Goniozus wasp populations (2014)
Journal Article
Khidr, S. K., Hardy, I. C., Zaviezo, T., & Mayes, S. (2014). Development of microsatellite markers and detection of genetic variation between Goniozus wasp populations. Journal of Insect Science, 14(43), https://doi.org/10.1673/031.014.43

Molecular genetic markers reveal differences between genotypes according to the presence of alleles (the same or different) at target loci. Microsatellite markers are especially useful codominant markers that have been used in a wide range of studies... Read More about Development of microsatellite markers and detection of genetic variation between Goniozus wasp populations.

Comparison of ambient solvent extraction methods for the analysis of fatty acids in non-starch lipids of flour and starch (2014)
Journal Article
Bahrami, N., Yonekura, L., Linforth, R. S., Carvalho da Silva, M., Hill, S., Penson, S., …Fisk, I. D. (2014). Comparison of ambient solvent extraction methods for the analysis of fatty acids in non-starch lipids of flour and starch. Journal of the Science of Food and Agriculture, 94(3), https://doi.org/10.1002/jsfa.6449

BACKGROUND: Lipids are minor components of flours, but are major determinants of baking properties and end-product quality. To the best of our knowledge, there is no single solvent system currently known that efficiently extracts all non-starch lipids... Read More about Comparison of ambient solvent extraction methods for the analysis of fatty acids in non-starch lipids of flour and starch.