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Ras GTPase-like protein MglA, a controller of bacterial social-motility in Myxobacteria, has evolved to control bacterial predation by Bdellovibrio

Milner, David S.; Till, Rob; Cadby, Ian; Lovering, Andrew L.; Basford, Sarah M.; Saxon, Emma B.; Liddell, Susan; Williams, Laura E.; Sockett, R. Elizabeth

Authors

David S. Milner

Rob Till

Ian Cadby

Andrew L. Lovering

Sarah M. Basford

Emma B. Saxon

Susan Liddell

Laura E. Williams

LIZ SOCKETT LIZ.SOCKETT@NOTTINGHAM.AC.UK
Professor of Bacterial Genetics



Abstract

Bdellovibrio bacteriovorus invade Gram-negative bacteria in a predatory process requiring Type IV pili (T4P) at a single invasive pole, and also glide on surfaces to locate prey. Ras-like G-protein MglA, working with MglB and RomR in the deltaproteobacterium Myxococcus xanthus, regulates adventurous gliding and T4P-mediated social motility at both M. xanthus cell poles. Our bioinformatic analyses suggested that the GTPase activating protein (GAP)-encoding gene mglB was lost in Bdellovibrio, but critical residues for MglABd GTP-binding are conserved. Deletion of mglABd abolished prey-invasion, but not gliding, and reduced T4P formation. MglABd interacted with a previously uncharacterised tetratricopeptide repeat (TPR) domain protein Bd2492, which we show localises at the single invasive pole and is required for predation. Bd2492 and RomR also interacted with cyclic-di-GMP-binding receptor CdgA, required for rapid prey-invasion. Bd2492, RomRBd and CdgA localize to the invasive pole and may facilitate MglA-docking. Bd2492 was encoded from an operon encoding a TamAB-like secretion system. The TamA protein and RomR were found, by gene deletion tests, to be essential for viability in both predatory and non-predatory modes. Control proteins, which regulate bipolar T4P-mediated social motility in swarming groups of deltaproteobacteria, have adapted in evolution to regulate the anti-social process of unipolar prey-invasion in the “lone-hunter” Bdellovibrio. Thus GTP-binding proteins and cyclic-di-GMP inputs combine at a regulatory hub, turning on prey-invasion and allowing invasion and killing of bacterial pathogens and consequent predatory growth of Bdellovibrio.

Citation

Milner, D. S., Till, R., Cadby, I., Lovering, A. L., Basford, S. M., Saxon, E. B., …Sockett, R. E. (2014). Ras GTPase-like protein MglA, a controller of bacterial social-motility in Myxobacteria, has evolved to control bacterial predation by Bdellovibrio. PLoS Genetics, 10(4), https://doi.org/10.1371/journal.pgen.1004253

Journal Article Type Article
Acceptance Date Feb 4, 2014
Publication Date Apr 10, 2014
Deposit Date Jul 6, 2016
Publicly Available Date Jul 6, 2016
Journal PLoS Genetics
Print ISSN 1553-7390
Electronic ISSN 1553-7404
Publisher Public Library of Science
Peer Reviewed Peer Reviewed
Volume 10
Issue 4
Article Number e1004253
DOI https://doi.org/10.1371/journal.pgen.1004253
Public URL http://eprints.nottingham.ac.uk/id/eprint/34672
Publisher URL http://journals.plos.org/plosgenetics/article?id=10.1371/journal.pgen.1004253
Copyright Statement Copyright information regarding this work can be found at the following address: http://creativecommons.org/licenses/by/4.0

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Copyright Statement
Copyright information regarding this work can be found at the following address: http://creativecommons.org/licenses/by/4.0





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