All Outputs (1271)

Discovery and Computational Modelling of Adsorbent Polymers that Effectively Immobilize SARS-CoV-2 with Potential Practical Applications (2024)
Journal Article
Xue, X., Duncan, J. D., Coleman, C. M., Contreas, L., Blackburn, C., Vivero-Lopez, M., Williams, P. M., Ball, J. K., Alexander, C., & Alexander, M. R. (2024). Discovery and Computational Modelling of Adsorbent Polymers that Effectively Immobilize SARS-CoV-2 with Potential Practical Applications. Cell Reports Physical Science, 5(9), Article 102204. https://doi.org/10.1016/j.xcrp.2024.102204

Viral translocation is considered a common way for respiratory viruses to spread and contaminate the surrounding environment. Thus, the discovery of non-eluting polymers that immobilize severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) upo... Read More about Discovery and Computational Modelling of Adsorbent Polymers that Effectively Immobilize SARS-CoV-2 with Potential Practical Applications.

The Discovery of Substituted 5-(2-Hydroxybenzoyl)-2-Pyridone Analogues as Inhibitors of the Human Caf1/CNOT7 Ribonuclease (2024)
Journal Article
Kaur, I., Jadhav, G. P., Fischer, P. M., & Winkler, G. S. (2024). The Discovery of Substituted 5-(2-Hydroxybenzoyl)-2-Pyridone Analogues as Inhibitors of the Human Caf1/CNOT7 Ribonuclease. Molecules, 29(18), Article 4351. https://doi.org/10.3390/molecules29184351

The Caf1/CNOT7 nuclease is a catalytic component of the Ccr4-Not deadenylase complex, which is a key regulator of post-transcriptional gene regulation. In addition to providing catalytic activity, Caf1/CNOT7 and its paralogue Caf1/CNOT8 also contribu... Read More about The Discovery of Substituted 5-(2-Hydroxybenzoyl)-2-Pyridone Analogues as Inhibitors of the Human Caf1/CNOT7 Ribonuclease.

Theoretical Study on the Biosynthesis of the Mandapamates: Mechanistic Insights Using Density Functional Theory (2024)
Journal Article
Wang, D., Pattenden, G., Fow, K. L., Stocks, M. J., Hirst, J. D., & Tang, B. (2024). Theoretical Study on the Biosynthesis of the Mandapamates: Mechanistic Insights Using Density Functional Theory. Journal of Organic Chemistry, https://doi.org/10.1021/acs.joc.4c00859

Density functional theory (B3LYP-D3(BJ) and ωB97XD) calculations have been used to assess the stereochemical outcomes of the proposed transannular [4 + 2] cycloaddition pathway for the biosynthesis of mandapamate and isomandapamate from macrocyclic i... Read More about Theoretical Study on the Biosynthesis of the Mandapamates: Mechanistic Insights Using Density Functional Theory.

Cannabidiol and fluorinated derivative anti-cancer properties against glioblastoma multiforme cell lines, and synergy with imidazotetrazine agents (2024)
Journal Article
Brookes, A., Kindon, N., Scurr, D. J., Alexander, M. R., Gershkovich, P., & Bradshaw, T. (2024). Cannabidiol and fluorinated derivative anti-cancer properties against glioblastoma multiforme cell lines, and synergy with imidazotetrazine agents. BJC Reports, 2, Article 67. https://doi.org/10.1038/s44276-024-00088-0

Background
Glioblastoma multiforme (GBM) is an aggressive cancer with poor prognosis, partly due to resistance to the standard chemotherapy treatment, temozolomide (TMZ). Phytocannabinoid cannabidiol (CBD) has exhibited anti-cancer effects against G... Read More about Cannabidiol and fluorinated derivative anti-cancer properties against glioblastoma multiforme cell lines, and synergy with imidazotetrazine agents.

Assessing the performance of machine learning methods trained on public health observational data: a case study from COVID-19 (2024)
Journal Article
Pigoli, D., Baker, K., Budd, J., Butler, L., Coppock, H., Egglestone, S., Gilmour, S., Holmes, C., Hurley, D., Jersakova, R., Kiskin, I., Koutra, V., Mellor, J., Nicholson, G., Packham, J., Patel, S., Roberts, S. J., Schuller, B. W., Tendero-Cañadas, A., Thornley, T., & Titcomb, A. (2024). Assessing the performance of machine learning methods trained on public health observational data: a case study from COVID-19. Statistics in Medicine, https://doi.org/10.1002/sim.10211

From early in the coronavirus disease 2019 (COVID-19) pandemic, there was interest in using machine learning methods to predict COVID-19 infection status based on vocal audio signals, for example, cough recordings. However, early studies had limitati... Read More about Assessing the performance of machine learning methods trained on public health observational data: a case study from COVID-19.

d-Xylose oxetane copolymers as bioderived and tuneable polyesters for amorphous solid dispersions (2024)
Journal Article
F. Clark, E., Howard, A., D. Morales Feliu, S., F. McCabe, J., C. Burley, J., Taresco, V., & Buchard, A. (2024). d-Xylose oxetane copolymers as bioderived and tuneable polyesters for amorphous solid dispersions. RSC Applied Polymers, https://doi.org/10.1039/D4LP00203B

The ring-opening copolymerisation of cyclic anhydrides with an oxetane derived from natural monosaccharide D-xylose has been used to synthesise fully biobased water soluble polyesters, which are able to stabilise the amorphous phases of nifedipine an... Read More about d-Xylose oxetane copolymers as bioderived and tuneable polyesters for amorphous solid dispersions.

In vivo analysis of hybrid hydrogels containing dual growth factor combinations, and skeletal stem cells under mechanical stimulation for bone repair (2024)
Journal Article
Gothard, D., Rotherham, M., Smith, E. L., Kanczler, J. M., Henstock, J., Wells, J. A., Roberts, C. A., Qutachi, O., Peto, H., Rashidi, H., Rojo, L., White, L. J., Stevens, M. M., El Haj, A. J., Rose, F. R., & Oreffo, R. O. (2024). In vivo analysis of hybrid hydrogels containing dual growth factor combinations, and skeletal stem cells under mechanical stimulation for bone repair. Mechanobiology in Medicine, 2(4), Article 100096. https://doi.org/10.1016/j.mbm.2024.100096

Bone tissue engineering requires a combination of materials, cells, growth factors and mechanical cues to recapitulate bone formation. In this study we evaluated hybrid hydrogels for minimally invasive bone formation by combining biomaterials with sk... Read More about In vivo analysis of hybrid hydrogels containing dual growth factor combinations, and skeletal stem cells under mechanical stimulation for bone repair.

Stable Platform for Mevalonate Bioproduction from CO2 (2024)
Journal Article
Garavaglia, M., McGregor, C., Bommareddy, R. R., Irorere, V., Arenas, C., Robazza, A., Minton, N. P., & Kovacs, K. (2024). Stable Platform for Mevalonate Bioproduction from CO2. ACS Sustainable Chemistry and Engineering, https://doi.org/10.1021/acssuschemeng.4c03561

Stable production of value-added products using a microbial chassis is pivotal for determining the industrial suitability of the engineered biocatalyst. Microbial cells often lose the multicopy expression plasmids during long-term cultivations. Owing... Read More about Stable Platform for Mevalonate Bioproduction from CO2.

Computer Vision for Substrate Detection in High‐Throughput Biomaterial Screens Using Bright‐Field Microscopy (2024)
Journal Article
Owen, R., Nasir, A., H. Amer, M., Nie, C., Xue, X., Burroughs, L., Denning, C., D. Wildman, R., A. Khan, F., R. Alexander, M., & R. A. J. Rose, F. (2024). Computer Vision for Substrate Detection in High‐Throughput Biomaterial Screens Using Bright‐Field Microscopy. Advanced Intelligent Systems, Article 2400573. https://doi.org/10.1002/aisy.202400573

High-throughput screening (HTS) can be used when ab initio information is unavailable for rational design of new materials, generating data on properties such as chemistry and topography that control cell behavior. Biomaterial screens are typically f... Read More about Computer Vision for Substrate Detection in High‐Throughput Biomaterial Screens Using Bright‐Field Microscopy.

Supramolecular hydrogels enable co-delivery of chemotherapeutics with synergistic efficacy against patient-derived glioblastoma cells and spheroids (2024)
Journal Article
Cavanagh, R. J., Baquain, S., Alexander, C., Scherman, O. A., & Rahman, R. (2024). Supramolecular hydrogels enable co-delivery of chemotherapeutics with synergistic efficacy against patient-derived glioblastoma cells and spheroids. RSC Advances, https://doi.org/10.1039/D4PM00177J

Drug combinations have been shown to be highly effective in many cancer therapies but the ratios of the individual drugs must be adjusted carefully and formulated appropriately to ensure synergistic action. Here we assessed combinations of doxorubici... Read More about Supramolecular hydrogels enable co-delivery of chemotherapeutics with synergistic efficacy against patient-derived glioblastoma cells and spheroids.

Advancing insights into microgravity induced muscle changes using Caenorhabditis elegans as a model organism (2024)
Journal Article
Beckett, L. J., Williams, P. M., Toh, L. S., Hessel, V., Gerstweiler, L., Fisk, I., Toronjo-Urquiza, L., & Chauhan, V. M. (2024). Advancing insights into microgravity induced muscle changes using Caenorhabditis elegans as a model organism. npj Microgravity, 10(1), Article 79. https://doi.org/10.1038/s41526-024-00418-z

Spaceflight presents significant challenges to the physiological state of living organisms. This can be due to the microgravity environment experienced during long-term space missions, resulting in alterations in muscle structure and function, such a... Read More about Advancing insights into microgravity induced muscle changes using Caenorhabditis elegans as a model organism.

PLGA-PEG-PLGA hydrogels induce cytotoxicity in conventional in vitro assays (2024)
Journal Article
Stewart, C. L., Hook, A. L., Zelzer, M., Marlow, M., & Piccinini, A. M. (2024). PLGA-PEG-PLGA hydrogels induce cytotoxicity in conventional in vitro assays. Cell Biochemistry and Function, 42(5), Article e4097. https://doi.org/10.1002/cbf.4097

We identified that PLGA-PEG-PLGA hydrogels, which have been used in human clinical trials and possess a demonstrable safety profile, induced significant cytotoxicity in conventional in vitro assays. This major contradiction may lead to inconsistent a... Read More about PLGA-PEG-PLGA hydrogels induce cytotoxicity in conventional in vitro assays.

Ligand-Directed Labeling of the Adenosine A1 Receptor in Living Cells (2024)
Journal Article
Comeo, E., Goulding, J., Lin, C.-Y., Groenen, M., Woolard, J., Kindon, N. D., Harwood, C. R., Platt, S., Briddon, S. J., Kilpatrick, L. E., Scammells, P. J., Hill, S. J., & Kellam, B. (2024). Ligand-Directed Labeling of the Adenosine A1 Receptor in Living Cells. Journal of Medicinal Chemistry, 67(14), 12099–12117. https://doi.org/10.1021/acs.jmedchem.4c00835

The study of protein function and dynamics in their native cellular environment is essential for progressing fundamental science. To overcome the requirement of genetic modification of the protein or the limitations of dissociable fluorescent ligands... Read More about Ligand-Directed Labeling of the Adenosine A1 Receptor in Living Cells.

Metabolomic and Proteomic Analysis of ApoE4-Carrying H4 Neuroglioma Cells in Alzheimer’s Disease Using OrbiSIMS and LC-MS/MS (2024)
Journal Article
Lu, L., Kotowska, A. M., Kern, S., Fang, M., Rudd, T. R., Alexander, M. R., Scurr, D. J., & Zhu, Z. (2024). Metabolomic and Proteomic Analysis of ApoE4-Carrying H4 Neuroglioma Cells in Alzheimer’s Disease Using OrbiSIMS and LC-MS/MS. Analytical Chemistry, https://doi.org/10.1021/acs.analchem.4c01201

Growing clinical evidence reveals that systematic molecular alterations in the brain occur 20 years before the onset of AD pathological features. Apolipoprotein E4 (ApoE4) is one of the most significant genetic risk factors for Alzheimer’s disease (A... Read More about Metabolomic and Proteomic Analysis of ApoE4-Carrying H4 Neuroglioma Cells in Alzheimer’s Disease Using OrbiSIMS and LC-MS/MS.

β-glycerophosphate, not low magnitude fluid shear stress, increases osteocytogenesis in the osteoblast-to-osteocyte cell line IDG-SW3 (2024)
Journal Article
Owen, R., Wittkowske, C., Lacroix, D., Perrault, C. M., & Reilly, G. C. (2024). β-glycerophosphate, not low magnitude fluid shear stress, increases osteocytogenesis in the osteoblast-to-osteocyte cell line IDG-SW3. Connective Tissue Research, https://doi.org/10.1080/03008207.2024.2375065

Aim
As osteoblasts deposit a mineralized collagen network, a subpopulation of these cells differentiates into osteocytes. Biochemical and mechanical stimuli, particularly fluid shear stress (FSS), are thought to regulate this, but their relative inf... Read More about β-glycerophosphate, not low magnitude fluid shear stress, increases osteocytogenesis in the osteoblast-to-osteocyte cell line IDG-SW3.

Identification of Predictive Biomarkers of Lameness in Transition Dairy Cows (2024)
Journal Article
Cardoso, A. S., Whitby, A., Green, M. J., Kim, D.-H., & Randall, L. V. (2024). Identification of Predictive Biomarkers of Lameness in Transition Dairy Cows. Animals, 14(14), Article 2030. https://doi.org/10.3390/ani14142030

The aim of this study was to identify with a high level of confidence metabolites previously identified as predictors of lameness and understand their biological relevance by carrying out pathway analyses. For the dairy cattle sector, lameness is a m... Read More about Identification of Predictive Biomarkers of Lameness in Transition Dairy Cows.

Pilot and quantitative evaluation of the TARGET acne toolkit by UK pharmacy professionals working in general practice (2024)
Journal Article
Sonnex, K., Thornley, T., Fleming, N., Lakha, A., Lecky, D. M., Pillay, I., …Boyd, M. (2024). Pilot and quantitative evaluation of the TARGET acne toolkit by UK pharmacy professionals working in general practice. BMJ Open, 14(7), Article e081641. https://doi.org/10.1136/bmjopen-2023-081641

Background: Acne vulgaris (acne) is a common skin condition sometimes needing topical or oral antibiotic treatment. Pharmacists and pharmacy technicians (together known as pharmacy professionals) working in general practice are well placed to ensure... Read More about Pilot and quantitative evaluation of the TARGET acne toolkit by UK pharmacy professionals working in general practice.

Discovery of MT-1207: A Novel, Potent Multitarget Inhibitor as a Promising Clinical Candidate for the Treatment of Hypertension (2024)
Journal Article
Wang, P., Zhu, H., Tian, J.-S., Zhu, W., Xu, S., Yao, H., Liu, J., Zhu, Z., Miao, C.-Y., & Xu, J. (2024). Discovery of MT-1207: A Novel, Potent Multitarget Inhibitor as a Promising Clinical Candidate for the Treatment of Hypertension. Journal of Medicinal Chemistry, https://doi.org/10.1021/acs.jmedchem.4c00626

Herein, a series of novel arylpiperazine (piperidine) derivatives were designed, synthesized, and evaluated for mechanisms of action through in vitro and in vivo studies. The most promising compound, II-13 (later named as MT-1207), is a potent α1 and... Read More about Discovery of MT-1207: A Novel, Potent Multitarget Inhibitor as a Promising Clinical Candidate for the Treatment of Hypertension.

Cellular and microenvironmental cues that promote macrophage fusion and foreign body response (2024)
Journal Article
Stewart, C. L., Marlow, M., Zelzer, M., Hook, A. L., & Piccinini, A. M. (in press). Cellular and microenvironmental cues that promote macrophage fusion and foreign body response. Frontiers in Immunology, 15, https://doi.org/10.3389/fimmu.2024.1411872

During the foreign body response (FBR), macrophages fuse to form foreign body giant cells (FBGCs). Modulation of FBGC formation can prevent biomaterial degradation and loss of therapeutic efficacy. However, the microenvironmental cues that dictate FB... Read More about Cellular and microenvironmental cues that promote macrophage fusion and foreign body response.

Targeting Macrophage Polarization for Reinstating Homeostasis following Tissue Damage (2024)
Journal Article
Du, Q., Dickinson, A., Nakuleswaran, P., Maghami, S., Alagoda, S., Hook, A. L., & Ghaemmaghami, A. M. (2024). Targeting Macrophage Polarization for Reinstating Homeostasis following Tissue Damage. International Journal of Molecular Sciences, 25(13), Article 7278. https://doi.org/10.3390/ijms25137278

Tissue regeneration and remodeling involve many complex stages. Macrophages are critical in maintaining micro-environmental homeostasis by regulating inflammation and orchestrating wound healing. They display high plasticity in response to various st... Read More about Targeting Macrophage Polarization for Reinstating Homeostasis following Tissue Damage.