Ligand-Directed Labeling of the Adenosine A1 Receptor in Living Cells

Comeo, Eleonora; Goulding, Joëlle; Lin, Chia-Yang; Groenen, Marleen; Woolard, Jeanette; Kindon, Nicholas D.; Harwood, Clare R.; Platt, Simon; Briddon, Stephen J.; Kilpatrick, Laura E.; Scammells, Peter J.; Hill, Stephen J.; Kellam, Barrie

Abstract

The study of protein function and dynamics in their native cellular environment is essential for progressing fundamental science. To overcome the requirement of genetic modification of the protein or the limitations of dissociable fluorescent ligands, ligand-directed (LD) chemistry has most recently emerged as a complementary, bioorthogonal approach for labeling native proteins. Here, we describe the rational design, development, and application of the first ligand-directed chemistry approach for labeling the A1AR in living cells. We pharmacologically demonstrate covalent labeling of A1AR expressed in living cells while the orthosteric binding site remains available. The probes were imaged using confocal microscopy and fluorescence correlation spectroscopy to study A1AR localization and dynamics in living cells. Additionally, the probes allowed visualization of the specific localization of A1ARs endogenously expressed in dorsal root ganglion (DRG) neurons. LD probes developed here hold promise for illuminating ligand-binding, receptor signaling, and trafficking of the A1AR in more physiologically relevant environments.

Citation

Comeo, E., Goulding, J., Lin, C.-Y., Groenen, M., Woolard, J., Kindon, N. D., Harwood, C. R., Platt, S., Briddon, S. J., Kilpatrick, L. E., Scammells, P. J., Hill, S. J., & Kellam, B. (2024). Ligand-Directed Labeling of the Adenosine A1 Receptor in Living Cells. Journal of Medicinal Chemistry, 67(14), 12099–12117. https://doi.org/10.1021/acs.jmedchem.4c00835

• Journal Article Type: Article
• Acceptance Date: Jun 24, 2024
• Online Publication Date: Jul 12, 2024
• Publication Date: Jul 25, 2024
• Deposit Date: Jul 13, 2024
• Publicly Available Date: Jul 16, 2024
• Journal: Journal of Medicinal Chemistry
• Print ISSN: 0022-2623
• Electronic ISSN: 1520-4804
• Publisher: American Chemical Society
• Peer Reviewed: Peer Reviewed
• Volume: 67
• Issue: 14
• Pages: 12099–12117
• DOI: https://doi.org/10.1021/acs.jmedchem.4c00835
• Public URL: https://nottingham-repository.worktribe.com/output/37158351
• Publisher URL: https://pubs.acs.org/doi/10.1021/acs.jmedchem.4c00835