All Outputs (20)

Quorum-sensing, intra- and inter-species competition in the staphylococci (2023)
Journal Article

In Gram-positive bacteria such as Staphylococcus aureus and the coagulase-negative staphylococci (CoNS), the accessory gene regulator (agr) is a highly conserved but polymorphic quorum-sensing system involved in colonization, virulence and biofilm de... Read More about Quorum-sensing, intra- and inter-species competition in the staphylococci.

Engineering nanowires in bacteria to elucidate electron transport structural–functional relationships (2023)
Journal Article

Bacterial pilin nanowires are protein complexes, suggested to possess electroactive capabilities forming part of the cells’ bioenergetic programming. Their role is thought to be linked to facilitating electron transfer between cells and the external... Read More about Engineering nanowires in bacteria to elucidate electron transport structural–functional relationships.

Conformational analysis and interaction of the Staphylococcus aureus transmembrane peptidase AgrB with its AgrD propeptide substrate (2023)
Journal Article

Virulence gene expression in the human pathogen, S. aureus is regulated by the agr (accessory gene regulator) quorum sensing (QS) system which is conserved in diverse Gram-positive bacteria. The agr QS signal molecule is an autoinducing peptide (AIP)... Read More about Conformational analysis and interaction of the Staphylococcus aureus transmembrane peptidase AgrB with its AgrD propeptide substrate.

Synthetic Biology Toolbox, Including a Single-Plasmid CRISPR-Cas9 System to Biologically Engineer the Electrogenic, Metal-Resistant Bacterium Cupriavidus metallidurans CH34 (2022)
Journal Article

Cupriavidus metallidurans CH34 exhibits extraordinary metabolic versatility, including chemolithoautotrophic growth; degradation of BTEX (benzene, toluene, ethylbenzene, xylene); high resistance to numerous metals; biomineralization of gold, platinum... Read More about Synthetic Biology Toolbox, Including a Single-Plasmid CRISPR-Cas9 System to Biologically Engineer the Electrogenic, Metal-Resistant Bacterium Cupriavidus metallidurans CH34.

Enhancing Microbial Electron Transfer through Synthetic Biology and Biohybrid Approaches: Part I: Bioelectrochemistry for sustainable energy conversion (2022)
Journal Article

Traditional microbial synthesis of chemicals and fuels often rely on energy-rich feedstocks such as glucose, raising ethical concerns as they are directly competing with the food supply. Therefore, it is imperative to develop novel processes that rel... Read More about Enhancing Microbial Electron Transfer through Synthetic Biology and Biohybrid Approaches: Part I: Bioelectrochemistry for sustainable energy conversion.

Oxygen-Tolerant RAFT Polymerization Initiated by Living Bacteria (2022)
Journal Article

Living organisms can synthesize a wide range of macromolecules from a small set of natural building blocks, yet there is potential for even greater materials diversity by exploiting biochemical processes to convert unnatural feedstocks into new abiot... Read More about Oxygen-Tolerant RAFT Polymerization Initiated by Living Bacteria.

Oxygen-Tolerant RAFT Polymerization Initiated by Living Bacteria (2022)
Journal Article

Living organisms can synthesize a wide range of macromolecules from a small set of natural building blocks, yet there is potential for even greater materials diversity by exploiting biochemical processes to convert unnatural feedstocks into new abiot... Read More about Oxygen-Tolerant RAFT Polymerization Initiated by Living Bacteria.

Engineering bacteria to control electron transport altering the synthesis of non-native polymer (2021)
Journal Article

The use of bacteria as catalysts for radical polymerisations of synthetic monomers has recently been established. However, the role of trans Plasma Membrane Electron Transport (tPMET) in modulating these processes is not well understood. We sort to s... Read More about Engineering bacteria to control electron transport altering the synthesis of non-native polymer.

Marine-Source Quorum Quenching Enzyme YtnP to Improve Hygiene Quality in Dental Units (2021)
Journal Article

Biofilm in dental unit water lines may pose a health risk to patients and dental practitioners. An AdiC-like quorum quenching enzyme, YtnP, was cloned from a deep-sea probiotic Bacillus velezensis, and heterologously expressed in E. coli to examine t... Read More about Marine-Source Quorum Quenching Enzyme YtnP to Improve Hygiene Quality in Dental Units.

Gaussia luciferase as a reporter for quorum sensing in staphylococcus aureus (2020)
Journal Article

© 2020 by the authors. Licensee MDPI, Basel, Switzerland. Gaussia luciferase (GLuc) is a secreted protein with significant potential for use as a reporter of gene expression in bacterial pathogenicity studies. To date there are relatively few example... Read More about Gaussia luciferase as a reporter for quorum sensing in staphylococcus aureus.

Development of a Corynebacterium glutamicum bio-factory for self-sufficient transaminase reactions (2020)
Journal Article

The development of biocatalytic routes for the synthesis of chiral amines starting from achiral building blocks is highly desirable. Here, we report a self-sufficient whole-cell system for the conversion of a model ketone to the corresponding cyclic... Read More about Development of a Corynebacterium glutamicum bio-factory for self-sufficient transaminase reactions.

Iron Catalysed Radical Polymerisation by Living Bacteria (2020)
Journal Article

The ability to harness cellular redox processes for abiotic synthesis might allow the preparation of engineered hybrid living systems. Towards this goal, we describe a new bacteria‐mediated Iron‐catalysed Reversible Deactivation Radical Polymerisatio... Read More about Iron Catalysed Radical Polymerisation by Living Bacteria.

Biosynthetic bifunctional enzyme complex with high-efficiency luciferin-recycling to enhance the bioluminescence imaging (2019)
Journal Article

Firefly luciferase is a prominent reporter on molecular imaging with the advantage of longer wavelength on light emission and the ATP linear correlation, which makes it useful in most of current bioluminescence imaging model. However, the utility of... Read More about Biosynthetic bifunctional enzyme complex with high-efficiency luciferin-recycling to enhance the bioluminescence imaging.

Fusion expression of bifunctional enzyme complex for luciferin-recycling to enhance the luminescence imaging (2018)
Journal Article

Firefly luciferase (Fluc) has been widely used as a bioluminescent monitor. The ATP linear correlation and exogenous luciferin requirement make it useful in most of current imaging systems. However, the utility of this reporter was still limited by t... Read More about Fusion expression of bifunctional enzyme complex for luciferin-recycling to enhance the luminescence imaging.

Ultrasound-mediation of self-illuminating reporters improves imaging resolution in optically scattering media (2018)
Journal Article

In vivo imaging of self-illuminating bio-and chemiluminescent reporters is used to observe the physiology of small animals. However, strong light scattering by biological tissues results in poor spatial resolution of the optical imaging, which also d... Read More about Ultrasound-mediation of self-illuminating reporters improves imaging resolution in optically scattering media.

Reconstructing promoter activity from Lux bioluminescent reporters (2017)
Journal Article

The bacterial Lux system is used as a gene expression reporter. It is fast, sensitive and non-destructive, enabling high frequency measurements. Originally developed for bacterial cells, it has also been adapted for eukaryotic cells, and can be used... Read More about Reconstructing promoter activity from Lux bioluminescent reporters.

Correction: Hypothalamic over-expression of VGF in the Siberian hamster increases energy expenditure and reduces body weight gain (PLoS ONE 12:2: (e0172724) DOI: 10.1371/journal.pone.0172724) (2017)
Journal Article

© 2017 Lewis et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are cred... Read More about Correction: Hypothalamic over-expression of VGF in the Siberian hamster increases energy expenditure and reduces body weight gain (PLoS ONE 12:2: (e0172724) DOI: 10.1371/journal.pone.0172724).

Hypothalamic over-expression of VGF in the Siberian hamster increases energy expenditure and reduces body weight gain (2017)
Journal Article

VGF (non-acronymic) was first highlighted to have a role in energy homeostasis through experiments involving dietary manipulation in mice. Fasting increased VGF mRNA in the Arc and levels were subsequently reduced upon refeeding. This anabolic role f... Read More about Hypothalamic over-expression of VGF in the Siberian hamster increases energy expenditure and reduces body weight gain.

Synthesis, in vitro evaluation, and radiolabeling of fluorinated puromycin analogues: potential candidates for PET imaging of protein synthesis (2016)
Journal Article

There is currently no ideal radiotracer for imaging protein synthesis rate (PSR) by positron emission tomography (PET). Existing fluorine-18 labelled amino acid-based radiotracers predominantly visualize amino acid transporter processes, and in many... Read More about Synthesis, in vitro evaluation, and radiolabeling of fluorinated puromycin analogues: potential candidates for PET imaging of protein synthesis.

The dynamic balance of import and export of zinc in Escherichia coli suggests a heterogeneous population response to stress (2015)
Journal Article

Zinc is essential for life, but toxic in excess. Thus all cells must control their internal zinc concentration. We used a systems approach, alternating rounds of experiments and models, to further elucidate the zinc control systems in Escherichia col... Read More about The dynamic balance of import and export of zinc in Escherichia coli suggests a heterogeneous population response to stress.