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Development of a Corynebacterium glutamicum bio-factory for self-sufficient transaminase reactions

Grigoriou, Stylianos; Kugler, Pierre; Kulcinskaja, Evelina; Walter, Frederik; King, John; Hill, Phil; Wendisch, Volker F.; O'Reilly, Elaine

Authors

Stylianos Grigoriou

Pierre Kugler

Evelina Kulcinskaja

Frederik Walter

JOHN KING john.king@nottingham.ac.uk
Professor of Theoretical Mechanics

PHIL HILL phil.hill@nottingham.ac.uk
Associate Professor

Volker F. Wendisch

Elaine O'Reilly



Abstract

The development of biocatalytic routes for the synthesis of chiral amines starting from achiral building blocks is highly desirable. Here, we report a self-sufficient whole-cell system for the conversion of a model ketone to the corresponding cyclic imine, in good isolated yield (42%) and excellent enantioselectivity (>99% ee). The Corynebacterium glutamicum host produces the transaminase biocatalyst, cofactor and ‘smart’ amine donor (cadaverine or putrescine) in vivo, and highlights the potential for producing high-value chemicals from readily available building blocks. The report represents the first example of the application of a metabolically engineered organism for the production of smart diamine donors and their application in a transaminase biotransformation.

Citation

Grigoriou, S., Kugler, P., Kulcinskaja, E., Walter, F., King, J., Hill, P., …O'Reilly, E. (2020). Development of a Corynebacterium glutamicum bio-factory for self-sufficient transaminase reactions. Green Chemistry, 22(13), 4128-4132. https://doi.org/10.1039/d0gc01432j

Journal Article Type Article
Acceptance Date Jun 8, 2020
Online Publication Date Jun 11, 2020
Publication Date Jul 7, 2020
Deposit Date Jun 11, 2020
Publicly Available Date Jun 12, 2021
Journal Green Chemistry
Print ISSN 1463-9262
Electronic ISSN 1463-9270
Publisher Royal Society of Chemistry
Peer Reviewed Peer Reviewed
Volume 22
Issue 13
Pages 4128-4132
DOI https://doi.org/10.1039/d0gc01432j
Keywords Pollution; Environmental Chemistry
Public URL https://nottingham-repository.worktribe.com/output/4625954
Publisher URL http://dx.doi.org/10.1039/D0GC01432J
Additional Information : This document is Similarity Check deposited; : Supplementary Information; : Volker F. Wendisch (ORCID); : Volker F. Wendisch (ResearcherID); : Elaine O'Reilly (ORCID); : Single-blind; : Received 2 May 2020; Accepted 8 June 2020; Accepted Manuscript published 11 June 2020; Advance Article published 17 June 2020; Version of Record published 6 July 2020

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