Development of a Corynebacterium glutamicum bio-factory for self-sufficient transaminase reactions

Grigoriou, Stylianos; Kugler, Pierre; Kulcinskaja, Evelina; Walter, Frederik; King, John; Hill, Phil; Wendisch, Volker F.; O'Reilly, Elaine

Abstract

The development of biocatalytic routes for the synthesis of chiral amines starting from achiral building blocks is highly desirable. Here, we report a self-sufficient whole-cell system for the conversion of a model ketone to the corresponding cyclic imine, in good isolated yield (42%) and excellent enantioselectivity (>99% ee). The Corynebacterium glutamicum host produces the transaminase biocatalyst, cofactor and ‘smart’ amine donor (cadaverine or putrescine) in vivo, and highlights the potential for producing high-value chemicals from readily available building blocks. The report represents the first example of the application of a metabolically engineered organism for the production of smart diamine donors and their application in a transaminase biotransformation.

Citation

Grigoriou, S., Kugler, P., Kulcinskaja, E., Walter, F., King, J., Hill, P., …O'Reilly, E. (2020). Development of a Corynebacterium glutamicum bio-factory for self-sufficient transaminase reactions. Green Chemistry, 22(13), 4128-4132. https://doi.org/10.1039/d0gc01432j

• Journal Article Type: Article
• Acceptance Date: Jun 8, 2020
• Online Publication Date: Jun 11, 2020
• Publication Date: Jul 7, 2020
• Deposit Date: Jun 11, 2020
• Publicly Available Date: Jun 12, 2021
• Journal: Green Chemistry
• Print ISSN: 1463-9262
• Electronic ISSN: 1463-9270
• Publisher: Royal Society of Chemistry
• Peer Reviewed: Peer Reviewed
• Volume: 22
• Issue: 13
• Pages: 4128-4132
• DOI: https://doi.org/10.1039/d0gc01432j
• Keywords: Pollution; Environmental Chemistry
• Public URL: https://nottingham-repository.worktribe.com/output/4625954
• Publisher URL: http://dx.doi.org/10.1039/D0GC01432J
• Additional Information: : This document is Similarity Check deposited; : Supplementary Information; : Volker F. Wendisch (ORCID); : Volker F. Wendisch (ResearcherID); : Elaine O'Reilly (ORCID); : Single-blind; : Received 2 May 2020; Accepted 8 June 2020; Accepted Manuscript published 11 June 2020; Advance Article published 17 June 2020; Version of Record published 6 July 2020