All Outputs (88)

Expanding the repertoire of gene tools for precise manipulation of the Clostridium difficile genome: allelic exchange using pyrE alleles (2013)
Journal Article
Ng, Y. K., Ehsaan, M., Philip, S., Collery, M. M., Janoir, C., Collignon, A., …Minton, N. P. (2013). Expanding the repertoire of gene tools for precise manipulation of the Clostridium difficile genome: allelic exchange using pyrE alleles. PLoS ONE, 8(2), Article 13. https://doi.org/10.1371/journal.pone.0056051

Sophisticated genetic tools to modify essential biological processes at the molecular level are pivotal in elucidating the molecular pathogenesis of Clostridium difficile, a major cause of healthcare associated disease. Here we have developed an effi... Read More about Expanding the repertoire of gene tools for precise manipulation of the Clostridium difficile genome: allelic exchange using pyrE alleles.

Multiple factors modulate biofilm formation by the anaerobic pathogen Clostridium difficile (2013)
Journal Article
Dapa, T., Leuzzi, R., Ng, Y. K., Baban, S. T., Adamo, R., Kuehne, S. A., …Unnikrishnan, M. (2013). Multiple factors modulate biofilm formation by the anaerobic pathogen Clostridium difficile. Journal of Bacteriology, 195(3), https://doi.org/10.1128/JB.01980-12

Bacteria within biofilms are protected from multiple stresses, including immune responses and antimicrobial agents. The biofilm-forming ability of bacterial pathogens has been associated with increased antibiotic resistance and chronic recurrent infe... Read More about Multiple factors modulate biofilm formation by the anaerobic pathogen Clostridium difficile.

Spores of Clostridium difficile clinical isolates display a diverse germination response to bile salts (2012)
Journal Article
Heeg, D., Burns, D. A., Cartman, S. T., & Minton, N. P. (2012). Spores of Clostridium difficile clinical isolates display a diverse germination response to bile salts. PLoS ONE, 7(2), Article e32381. https://doi.org/10.1371/journal.pone.0032381

Clostridium difficile spores play a pivotal role in the transmission of infectious diarrhoea, but in order to cause disease spores must complete germination and return to vegetative cell growth. While the mechanisms of spore germination are well unde... Read More about Spores of Clostridium difficile clinical isolates display a diverse germination response to bile salts.

Integration of DNA into bacterial chromosomes from plasmids without a counter-selection marker (2012)
Journal Article
Heap, J. T., Ehsaan, M., Cooksley, C. M., Ng, Y., Cartman, S. T., Winzer, K., & Minton, N. P. (2012). Integration of DNA into bacterial chromosomes from plasmids without a counter-selection marker. Nucleic Acids Research, 40(8), Article 10. https://doi.org/10.1093/nar/gkr1321

Most bacteria can only be transformed with circular plasmids, so robust DNA integration methods for these rely upon selection of single-crossover clones followed by counter-selection of double-crossover clones. To overcome the limited availability of... Read More about Integration of DNA into bacterial chromosomes from plasmids without a counter-selection marker.

Both, toxin A and toxin B, are important in Clostridium difficile infection (2011)
Journal Article
Kuehne, S. A., Cartman, S. T., & Minton, N. P. (2011). Both, toxin A and toxin B, are important in Clostridium difficile infection. Gut Microbes, 2(4), https://doi.org/10.4161/gmic.2.4.16109

The bacterium Clostridium difficile is the leading cause of healthcare associated diarrhoea in the developed world and thus presents a major financial burden. The main virulence factors of C. difficile are two large toxins, A and B. Over the years th... Read More about Both, toxin A and toxin B, are important in Clostridium difficile infection.

Regulation of neurotoxin production and sporulation by a putative agrBD signaling system in proteolytic Clostridium botulinum (2010)
Journal Article
Cooksley, C. M., Davis, I. J., Winzer, K., Chan, W. C., Peck, M. W., & Minton, N. P. (2010). Regulation of neurotoxin production and sporulation by a putative agrBD signaling system in proteolytic Clostridium botulinum. Applied and Environmental Microbiology, 76(13), 4448-4460. https://doi.org/10.1128/AEM.03038-09

A significant number of genome sequences of Clostridium botulinum and related species have now been determined. In silico analysis of these data revealed the presence of two distinct agr loci (agr-1 and agr-2) in all group I strains, each encoding pu... Read More about Regulation of neurotoxin production and sporulation by a putative agrBD signaling system in proteolytic Clostridium botulinum.

Two-component signal transduction system CBO0787/ CBO0786 represses transcription from botulinum neurotoxin promoters in Clostridium botulinum ATCC 3502
Journal Article
Zhang, Z., Korkeala, H., Dahlsten, E., Sahala, E., Heap, J. T., Minton, N. P., & Linström, M. Two-component signal transduction system CBO0787/ CBO0786 represses transcription from botulinum neurotoxin promoters in Clostridium botulinum ATCC 3502. PLoS Pathogens, 9(3), Article e1003252. https://doi.org/10.1371/journal.ppat.1003252

Blocking neurotransmission, botulinum neurotoxin is the most poisonous biological substance known to mankind. Despite its infamy as the scourge of the food industry, the neurotoxin is increasingly used as a pharmaceutical to treat an expanding range... Read More about Two-component signal transduction system CBO0787/ CBO0786 represses transcription from botulinum neurotoxin promoters in Clostridium botulinum ATCC 3502.

Regulation of cel genes of C. cellulolyticum: identification of GlyR2, a transcriptional regulator regulating cel5D gene expression
Journal Article
Fendri, I., Abdou, L., Trotter, V., Dedieu, L., Maamar, H., Minton, N. P., & Tardif, C. Regulation of cel genes of C. cellulolyticum: identification of GlyR2, a transcriptional regulator regulating cel5D gene expression. PLoS ONE, 8(1), Article e44708. https://doi.org/10.1371/journal.pone.0044708

Transcription and expression regulation of some individual cel genes (cel5A, cel5I, cel5D and cel44O) of Clostridium cellulolyticum were investigated. Unlike the cip-cel operon, these genes are transcribed as monocistronic units of transcription, exc... Read More about Regulation of cel genes of C. cellulolyticum: identification of GlyR2, a transcriptional regulator regulating cel5D gene expression.