All Outputs (119)

Discovery of synergistic material-topography combinations to achieve immunomodulatory osteoinductive biomaterials using a novel in vitro screening method: The ChemoTopoChip (2021)
Journal Article
Burroughs, L., Amer, M., Vassey, M., Koch, B., Figueredo, G., Mukonoweshuro, B., Mikulskis, P., Vasilevich, A., Vermeulen, S., Dryden, I. L., Winkler, D. A., Ghaemmaghami, A. M., Rose, F. R. A. J., de Boer, J., & Alexander, M. R. (2021). Discovery of synergistic material-topography combinations to achieve immunomodulatory osteoinductive biomaterials using a novel in vitro screening method: The ChemoTopoChip. Biomaterials, 271, Article 120740. https://doi.org/10.1016/j.biomaterials.2021.120740

© 2021 The Authors Human mesenchymal stem cells (hMSCs) are widely represented in regenerative medicine clinical strategies due to their compatibility with autologous implantation. Effective bone regeneration involves crosstalk between macrophages an... Read More about Discovery of synergistic material-topography combinations to achieve immunomodulatory osteoinductive biomaterials using a novel in vitro screening method: The ChemoTopoChip.

AbaM Regulates Quorum Sensing, Biofilm Formation and Virulence in Acinetobacter baumannii (2021)
Journal Article
López-Martín, M., Dubern, J.-F., Alexander, M. R., & Williams, P. (2021). AbaM Regulates Quorum Sensing, Biofilm Formation and Virulence in Acinetobacter baumannii. Journal of Bacteriology, 203(8), Article e00635-20. https://doi.org/10.1128/jb.00635-20

Acinetobacter baumannii possesses a single divergent luxR/luxI-type quorum sensing (QS) locus named abaR/abaI. This locus also contains a third gene located between abaR and abaI which we term abaM that codes for an uncharacterized member of the RsaM... Read More about AbaM Regulates Quorum Sensing, Biofilm Formation and Virulence in Acinetobacter baumannii.

Polymer microarrays rapidly identify competitive adsorbents of virus-like particles (VLPs) (2020)
Journal Article
Blok, A. J., Gurnani, P., Xenopoulos, A., Burroughs, L., Duncan, J., Urbanowicz, R. A., Tsoleridis, T., Müller, H., Strecker, T., Ball, J. K., Alexander, C., & Alexander, M. R. (2020). Polymer microarrays rapidly identify competitive adsorbents of virus-like particles (VLPs). Biointerphases, 15, Article 061005. https://doi.org/10.1116/6.0000586

The emergence of SARS-CoV-2 highlights the global need for platform technologies to enable rapid development of diagnostics, vaccines, treatments, and personal protective equipment (PPE). However, many current technologies require the detailed mechan... Read More about Polymer microarrays rapidly identify competitive adsorbents of virus-like particles (VLPs).

Protein identification by 3D OrbiSIMS to facilitate in situ imaging and depth profiling (2020)
Journal Article
Kotowska, A. M., Trindade, G. F., Mendes, P. M., Williams, P. M., Aylott, J. W., Shard, A. G., Alexander, M. R., & Scurr, D. J. (2020). Protein identification by 3D OrbiSIMS to facilitate in situ imaging and depth profiling. Nature Communications, 11(1), Article 5832. https://doi.org/10.1038/s41467-020-19445-x

Label-free protein characterization at surfaces is commonly achieved using digestion and/or matrix application prior to mass spectrometry. We report the assignment of undigested proteins at surfaces in situ using secondary ion mass spectrometry (SIMS... Read More about Protein identification by 3D OrbiSIMS to facilitate in situ imaging and depth profiling.

Polymer microarrays rapidly identify competitive adsorbents of virus-like particles (2020)
Journal Article
Blok, A. J., Gurnani, P., Xenopoulos, A., Burroughs, L., Duncan, J., Urbanowicz, R. A., Tsoleridis, T., Müller-Kräuter, H., Strecker, T., Ball, J. K., Alexander, C., & Alexander, M. R. (2020). Polymer microarrays rapidly identify competitive adsorbents of virus-like particles. Biointerphases, 15(6), Article 061005. https://doi.org/10.1116/6.0000586

The emergence of SARS-CoV-2 highlights the global need for platform technologies to enable the rapid development of diagnostics, vaccines, treatments, and personal protective equipment (PPE). However, many current technologies require the detailed me... Read More about Polymer microarrays rapidly identify competitive adsorbents of virus-like particles.

Single-Cell Tracking on Polymer Microarrays Reveals the Impact of Surface Chemistry on Pseudomonas aeruginosa Twitching Speed and Biofilm Development (2020)
Journal Article
Carabelli, A. M., Isgró, M., Sanni, O., Figueredo, G. P., Winkler, D. A., Burroughs, L., Blok, A. J., Dubern, J. F., Pappalardo, F., Hook, A. L., Williams, P., & Alexander, M. R. (2020). Single-Cell Tracking on Polymer Microarrays Reveals the Impact of Surface Chemistry on Pseudomonas aeruginosa Twitching Speed and Biofilm Development. ACS Applied Bio Materials, 3(12), 8471–8480. https://doi.org/10.1021/acsabm.0c00849

© 2020 American Chemical Society. Bacterial biofilms exhibit up to 1000 times greater resistance to antibiotic or host immune clearance than planktonic cells. Pseudomonas aeruginosa produces retractable type IV pili (T4P) that facilitate twitching mo... Read More about Single-Cell Tracking on Polymer Microarrays Reveals the Impact of Surface Chemistry on Pseudomonas aeruginosa Twitching Speed and Biofilm Development.

Designing topographically textured microparticles for induction and modulation of osteogenesis in mesenchymal stem cell engineering (2020)
Journal Article
Amer, M. H., Alvarez-Paino, M., McLaren, J., Pappalardo, F., Trujillo, S., Wong, J. Q., Shrestha, S., Abdelrazig, S., Stevens, L. A., Lee, J. B., Kim, D. H., González-García, C., Needham, D., Salmerón-Sánchez, M., Shakesheff, K. M., Alexander, M. R., Alexander, C., & Rose, F. R. (2021). Designing topographically textured microparticles for induction and modulation of osteogenesis in mesenchymal stem cell engineering. Biomaterials, 266, Article 120450. https://doi.org/10.1016/j.biomaterials.2020.120450

© 2020 The Authors Mesenchymal stem cells are the focus of intense research in bone development and regeneration. The potential of microparticles as modulating moieties of osteogenic response by utilizing their architectural features is demonstrated... Read More about Designing topographically textured microparticles for induction and modulation of osteogenesis in mesenchymal stem cell engineering.

All Surfaces Are Not Equal in Contact Transmission of SARS-CoV-2 (2020)
Journal Article
Xue, X., Ball, J. K., Alexander, C., & Alexander, M. R. (2020). All Surfaces Are Not Equal in Contact Transmission of SARS-CoV-2. Matter, 3(5), 1433-1441. https://doi.org/10.1016/j.matt.2020.10.006

© 2020 Elsevier Inc. The world faces a severe and acute public health emergency due to the ongoing coronavirus disease 2019 (COVID-19) global pandemic caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Healthcare workers are... Read More about All Surfaces Are Not Equal in Contact Transmission of SARS-CoV-2.

Developing immune-regulatory materials using immobilized monosaccharides with immune-instructive properties (2020)
Journal Article
Alobaid, M. A., Richards, S.-J., Alexander, M. R., Gibson, M. I., & Ghaemmaghami, A. M. (2020). Developing immune-regulatory materials using immobilized monosaccharides with immune-instructive properties. Materials Today Bio, 8, Article 100080. https://doi.org/10.1016/j.mtbio.2020.100080

© 2020 The Authors New strategies for immune modulation have shown real promise in regenerative medicine as well as the fight against autoimmune diseases, allergies, and cancer. Dendritic cells (DCs) are gatekeepers of the immune system and their abi... Read More about Developing immune-regulatory materials using immobilized monosaccharides with immune-instructive properties.

Inkjet based 3D Printing of bespoke medical devices that resist bacterial biofilm formation (2020)
Preprint / Working Paper
He, Y., Begines, B., Luckett, J., Dubern, J.-F., Hook, A., Prina, E., Rose, F. R., Tuck, C., Hague, R., Irvine, D., Williams, P., Alexander, M. R., & Wildman, R. D. Inkjet based 3D Printing of bespoke medical devices that resist bacterial biofilm formation

We demonstrate the formulation of advanced functional 3D printing inks that prevent the formation of bacterial biofilms in vivo. Starting from polymer libraries, we show that a biofilm resistant object can be 3D printed with the potential for shape a... Read More about Inkjet based 3D Printing of bespoke medical devices that resist bacterial biofilm formation.

Achieving Microparticles with Cell-Instructive Surface Chemistry by Using Tunable Co-Polymer Surfactants (2020)
Journal Article
Dundas, A. A., Cuzzucoli Crucitti, V., Haas, S., Dubern, J., Latif, A., Romero, M., Sanni, O., Ghaemmaghami, A. M., Williams, P., Alexander, M. R., Wildman, R., & Irvine, D. J. (2020). Achieving Microparticles with Cell-Instructive Surface Chemistry by Using Tunable Co-Polymer Surfactants. Advanced Functional Materials, 30(36), Article 2001821. https://doi.org/10.1002/adfm.202001821

© 2020 The Authors. Published by WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim A flow-focusing microfluidic device is used to produce functionalized monodisperse polymer particles with surface chemistries designed to control bacterial biofilm formatio... Read More about Achieving Microparticles with Cell-Instructive Surface Chemistry by Using Tunable Co-Polymer Surfactants.

Discovery of (meth)acrylate polymers that resist colonization by fungi associated with pathogenesis and biodeterioration (2020)
Journal Article
Vallieres, C., Hook, A. L., He, Y., Crucitti, V. C., Figueredo, G., Davies, C. R., Burroughs, L., Winkler, D. A., Wildman, R. D., Irvine, D. J., Alexander, M. R., & Avery, S. V. (2020). Discovery of (meth)acrylate polymers that resist colonization by fungi associated with pathogenesis and biodeterioration. Science Advances, 6(23), Article eaba6574. https://doi.org/10.1126/sciadv.aba6574

© 2020 The Authors. Fungi have major, negative socioeconomic impacts, but control with bioactive agents is increasingly restricted, while resistance is growing. Here, we describe an alternative fungal control strategy via materials operating passivel... Read More about Discovery of (meth)acrylate polymers that resist colonization by fungi associated with pathogenesis and biodeterioration.

ToF-SIMS and Machine Learning for Single-Pixel Molecular Discrimination of an Acrylate Polymer Microarray (2020)
Journal Article
Gardner, W., Hook, A. L., Alexander, M. R., Ballabio, D., Cutts, S. M., Muir, B. W., & Pigram, P. J. (2020). ToF-SIMS and Machine Learning for Single-Pixel Molecular Discrimination of an Acrylate Polymer Microarray. Analytical Chemistry, 92(9), 6587-6597. https://doi.org/10.1021/acs.analchem.0c00349

© 2020 American Chemical Society. Combinatorial approaches to materials discovery offer promising potential for the rapid development of novel polymer systems. Polymer microarrays enable the high-throughput comparison of material physical and chemica... Read More about ToF-SIMS and Machine Learning for Single-Pixel Molecular Discrimination of an Acrylate Polymer Microarray.

Real time monitoring of biofilm formation on coated medical devices for the reduction and interception of bacterial infections (2020)
Journal Article
Kurmoo, Y., Hook, A. L., Harvey, D., Dubern, J.-F., Williams, P., Morgan, S. P., Korposh, S., & Alexander, M. R. (2020). Real time monitoring of biofilm formation on coated medical devices for the reduction and interception of bacterial infections. Biomaterials Science, 8(5), 1464-1477. https://doi.org/10.1039/c9bm00875f

Real time monitoring of bacterial attachment to medical devices provides opportunities to detect early biofilm formation and instigate appropriate interventions before infection develops. This study utilises long period grating (LPG) optical fibre se... Read More about Real time monitoring of biofilm formation on coated medical devices for the reduction and interception of bacterial infections.

Making tablets for delivery of poorly soluble drugs using photoinitiated 3D inkjet printing (2019)
Journal Article
Clark, E. A., Alexander, M. R., Irvine, D. J., Roberts, C. J., Wallace, M. J., Yoo, J., & Wildman, R. D. (2020). Making tablets for delivery of poorly soluble drugs using photoinitiated 3D inkjet printing. International Journal of Pharmaceutics, 578, Article 118805. https://doi.org/10.1016/j.ijpharm.2019.118805

© 2019 In this study, we investigate the viability of three-dimensional (3D) inkjet printing with UV curing to produce solid dosage forms containing a known poorly soluble drug, carvedilol. The formulation consists of 10 wt% carvedilol, Irgacure 2959... Read More about Making tablets for delivery of poorly soluble drugs using photoinitiated 3D inkjet printing.

Validating a Predictive Structure-Property Relationship by Discovery of Novel Polymers which Reduce Bacterial Biofilm Formation (2019)
Journal Article
Dundas, A. A., Sanni, O., Dubern, J.-F., Dimitrakis, G., Hook, A. L., Irvine, D. J., Williams, P., & Alexander, M. R. (2019). Validating a Predictive Structure-Property Relationship by Discovery of Novel Polymers which Reduce Bacterial Biofilm Formation. Advanced Materials, 31(49), Article 1903513. https://doi.org/10.1002/adma.201903513

ynthetic materials are an everyday component of modern healthcare yet often fail routinely as a consequence of medical‐device‐centered infections. The incidence rate for catheter‐associated urinary tract infections is between 3% and 7% for each day o... Read More about Validating a Predictive Structure-Property Relationship by Discovery of Novel Polymers which Reduce Bacterial Biofilm Formation.

Toward Interpretable Machine Learning Models for Materials Discovery (2019)
Journal Article
Mikulskis, P., Alexander, M. R., & Winkler, D. A. (2019). Toward Interpretable Machine Learning Models for Materials Discovery. Advanced Intelligent Systems, 1(8), Article 1900045. https://doi.org/10.1002/aisy.201900045

Machine learning (ML) and artificial intelligence (AI) methods for modeling useful materials properties are now important technologies for rational design and optimization of bespoke functional materials. Although these methods make good predictions... Read More about Toward Interpretable Machine Learning Models for Materials Discovery.

Simultaneous Tracking of Pseudomonas aeruginosa motility in liquid and at the Solid-Liquid Interface Reveals Differential Roles for the Flagellar Stators (2019)
Journal Article
Hook, A. L., Flewellen, J. L., Dubern, J.-F., Carabelli, A., Zald, I. M., Berry, R. M., Wildman, R. D., Russell, N., Williams, P., & Alexander, M. R. (2019). Simultaneous Tracking of Pseudomonas aeruginosa motility in liquid and at the Solid-Liquid Interface Reveals Differential Roles for the Flagellar Stators. mSystems, 4(5), Article e00390-19. https://doi.org/10.1128/mSystems.00390-19

Bacteria sense chemicals, surfaces, and other cells and move toward some and away from others. Studying how single bacterial cells in a population move requires sophisticated tracking and imaging techniques. We have established quantitative methodolo... Read More about Simultaneous Tracking of Pseudomonas aeruginosa motility in liquid and at the Solid-Liquid Interface Reveals Differential Roles for the Flagellar Stators.

Wireless Nanobioelectronics for Electrical Intracellular Sensing (2019)
Journal Article
Sanjuan-Alberte, P., Jain, A., Shaw, A. J., Abayzeed, S. A., Domínguez, R. F., Alea-Reyes, M. E., Clark, M., Alexander, M. R., Hague, R. J. M., Pérez-García, L., & Rawson, F. J. (2019). Wireless Nanobioelectronics for Electrical Intracellular Sensing. ACS Applied Nano Materials, 2(10), 6397-6408. https://doi.org/10.1021/acsanm.9b01374

For the field of bioelectronics to make an impact on healthcare, there is an urgent requirement for the development of “wireless” electronic systems to enable modulation of chemistry inside of cells. Herein we report on an intracellular wireless elec... Read More about Wireless Nanobioelectronics for Electrical Intracellular Sensing.

Dual Bioresponsive Antibiotic and Quorum Sensing Inhibitor Combination Nanoparticles for Treatment of Pseudomonas aeruginosa Biofilms In Vitro and Ex Vivo (2019)
Journal Article
Singh, N., Romero, M., Travanut, A., Monteiro, P., Jordana-Lluch, E., Hardie, K. R., Williams, P., Alexander, M., & Alexander, C. (2019). Dual Bioresponsive Antibiotic and Quorum Sensing Inhibitor Combination Nanoparticles for Treatment of Pseudomonas aeruginosa Biofilms In Vitro and Ex Vivo. Biomaterials Science, 10(7), 4099-4111. https://doi.org/10.1039/C9BM00773C

Many debilitating infections result from persistent microbial biofilms that do not respond to conventional antibiotic regimens. A potential method to treat such chronic infections is to combine agents which interfere with bacterial biofilm developmen... Read More about Dual Bioresponsive Antibiotic and Quorum Sensing Inhibitor Combination Nanoparticles for Treatment of Pseudomonas aeruginosa Biofilms In Vitro and Ex Vivo.