Mahetab H. Amer
Designing topographically textured microparticles for induction and modulation of osteogenesis in mesenchymal stem cell engineering
Amer, Mahetab H.; Alvarez-Paino, Marta; McLaren, Jane; Pappalardo, Francesco; Trujillo, Sara; Wong, Jing Qian; Shrestha, Sumana; Abdelrazig, Salah; Stevens, Lee A.; Lee, Jong Bong; Kim, Dong Hyun; Gonz�lez-Garc�a, Cristina; Needham, David; Salmer�n-S�nchez, Manuel; Shakesheff, Kevin M.; Alexander, Morgan R.; Alexander, Cameron; Rose, Felicity R.A.J.
Authors
Marta Alvarez-Paino
JANE MCLAREN jane.mclaren@nottingham.ac.uk
Nottingham Senior Tissue Bank Manager
Francesco Pappalardo
Sara Trujillo
Jing Qian Wong
Sumana Shrestha
Salah Abdelrazig
LEE STEVENS LEE.STEVENS@NOTTINGHAM.AC.UK
Senior Research Fellow
Jong Bong Lee
DONG-HYUN KIM Dong-hyun.Kim@nottingham.ac.uk
Associate Professor
Cristina Gonz�lez-Garc�a
DAVID NEEDHAM David.Needham@nottingham.ac.uk
Professor of Translational Therapeutics
Manuel Salmer�n-S�nchez
Kevin M. Shakesheff
MORGAN ALEXANDER MORGAN.ALEXANDER@NOTTINGHAM.AC.UK
Professor of Biomedical Surfaces
Professor CAMERON ALEXANDER CAMERON.ALEXANDER@NOTTINGHAM.AC.UK
Professor of Polymer Therapeutics
FELICITY ROSE FELICITY.ROSE@NOTTINGHAM.AC.UK
Professor of Biomaterials and Tissue Engineering
Abstract
© 2020 The Authors Mesenchymal stem cells are the focus of intense research in bone development and regeneration. The potential of microparticles as modulating moieties of osteogenic response by utilizing their architectural features is demonstrated herein. Topographically textured microparticles of varying microscale features are produced by exploiting phase-separation of a readily soluble sacrificial component from polylactic acid. The influence of varying topographical features on primary human mesenchymal stem cell attachment, proliferation and markers of osteogenesis is investigated. In the absence of osteoinductive supplements, cells cultured on textured microparticles exhibit notably increased expression of osteogenic markers relative to conventional smooth microparticles. They also exhibit varying morphological, attachment and proliferation responses. Significantly altered gene expression and metabolic profiles are observed, with varying histological characteristics in vivo. This study highlights how tailoring topographical design offers cell-instructive 3D microenvironments which allow manipulation of stem cell fate by eliciting the desired downstream response without use of exogenous osteoinductive factors.
Citation
Amer, M. H., Alvarez-Paino, M., McLaren, J., Pappalardo, F., Trujillo, S., Wong, J. Q., …Rose, F. R. (2021). Designing topographically textured microparticles for induction and modulation of osteogenesis in mesenchymal stem cell engineering. Biomaterials, 266, Article 120450. https://doi.org/10.1016/j.biomaterials.2020.120450
Journal Article Type | Article |
---|---|
Acceptance Date | Oct 10, 2020 |
Online Publication Date | Oct 12, 2020 |
Publication Date | Jan 1, 2021 |
Deposit Date | Oct 16, 2020 |
Publicly Available Date | Oct 16, 2020 |
Journal | Biomaterials |
Print ISSN | 0142-9612 |
Electronic ISSN | 1878-5905 |
Publisher | Elsevier |
Peer Reviewed | Peer Reviewed |
Volume | 266 |
Article Number | 120450 |
DOI | https://doi.org/10.1016/j.biomaterials.2020.120450 |
Keywords | Biophysics; Mechanics of Materials; Bioengineering; Biomaterials; Ceramics and Composites |
Public URL | https://nottingham-repository.worktribe.com/output/4967413 |
Publisher URL | https://www.sciencedirect.com/science/article/pii/S0142961220306967?via%3Dihub |
Files
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Publisher Licence URL
https://creativecommons.org/licenses/by/4.0/
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