All Outputs (45)

Study on molecular orientation and stratification in RNA-lipid nanoparticles by cryogenic orbitrap secondary ion mass spectrometry (2025)
Journal Article
Kotowska, A. M., Fay, M., Watts, J. A., Gilmore, I. S., Scurr, D. J., Howe, A., Capka, V., Perez, C., Doud, D., Patel, S., Umbarger, M., Langer, R., & Alexander, M. R. (2025). Study on molecular orientation and stratification in RNA-lipid nanoparticles by cryogenic orbitrap secondary ion mass spectrometry. Communications Chemistry, 8, Article 160. https://doi.org/10.1038/s42004-025-01526-x

Lipid nanoparticle RNA (LNP-RNA) formulations are used for the delivery of vaccines and other therapies. RNA molecules are encapsulated within their interior through electrostatic interactions with positively charged lipids. The identity of the lipid... Read More about Study on molecular orientation and stratification in RNA-lipid nanoparticles by cryogenic orbitrap secondary ion mass spectrometry.

Modulation of the biological response to surfaces through the controlled deposition of 3D polymeric surfactants (2025)
Journal Article
Cuzzucoli Crucitti, V., Hajiali, H., Dundas, A. A., Jayawarna, V., Tomolillo, D., Francolini, I., Vuotto, C., Salmeron-Sanchez, M., Dalby, M. J., Alexander, M. R., Wildman, R. D., Rose, F. R. A. J., & Irvine, D. J. (2025). Modulation of the biological response to surfaces through the controlled deposition of 3D polymeric surfactants. Journal of Materials Chemistry B, 13(15), 4657-4670. https://doi.org/10.1039/d4tb01941e

Biomaterials play a crucial role in modern medicine through their use as medical implants and devices. However, they can support biofilm formation and infection, and lack integration with the surrounding human tissue at the implant site. This work re... Read More about Modulation of the biological response to surfaces through the controlled deposition of 3D polymeric surfactants.

Molecular orientation and stratification revealed in RNA-lipid nanoparticles using Cryogenic Orbitrap Secondary Ion Mass Spectrometry (Cryo-OrbiSIMS) depth profiling (2025)
Preprint / Working Paper
Kotowska, A., Fay, M., Watts, J., Gilmore, I., Scurr, D., Howe, A., Capka, V., Perez, C., Doud, D., Patel, S., Umbarger, M., Langer, R., & Alexander, M. (2025). Molecular orientation and stratification revealed in RNA-lipid nanoparticles using Cryogenic Orbitrap Secondary Ion Mass Spectrometry (Cryo-OrbiSIMS) depth profiling

Lipid nanoparticle RNA (LNP-RNA) formulations are used for the delivery of vaccines and other therapies. RNA molecules are encapsulated within their interior through electrostatic interactions with positively charged lipids. The identity of the lipid... Read More about Molecular orientation and stratification revealed in RNA-lipid nanoparticles using Cryogenic Orbitrap Secondary Ion Mass Spectrometry (Cryo-OrbiSIMS) depth profiling.

Development of C. albican Anti-attachment Inkjet 3D Printing Ink, via High Throughput Screening (2025)
Presentation / Conference Contribution
Yong, L. X., Zhou, Z., Vallières, C., He, Y., Cuzzucoli Crucitti, V., Alexander, M. R., Avery, S., Wildman, R., & Irvine, D. (2024, August). Development of C. albican Anti-attachment Inkjet 3D Printing Ink, via High Throughput Screening. Presented at International Conference on Computational & Experimental Engineering and Sciences, Singapore, Singapore

The fungal pathogen Candida albicans (C. albicans) is particularly problematic for immunocompromised patients and those with medical implants. Introducing Candida-resistant medical devices could potentially reduce mortality rates from such infections... Read More about Development of C. albican Anti-attachment Inkjet 3D Printing Ink, via High Throughput Screening.

Fungal Attachment-Resistant Polymers for the Additive Manufacture of Medical Devices (2024)
Journal Article
Yong, L. X., Sefton, J., Vallières, C., Rance, G. A., Hill, J., Cuzzucoli Crucitti, V., Dundas, A. A., Rose, F. R., Alexander, M. R., Wildman, R., He, Y., Avery, S. V., & Irvine, D. J. (2024). Fungal Attachment-Resistant Polymers for the Additive Manufacture of Medical Devices. ACS Applied Materials and Interfaces, 16(40), 54508–54519. https://doi.org/10.1021/acsami.4c04833

This study reports the development of the first copolymer material that (i) is resistant to fungal attachment and hence biofilm formation, (ii) operates via a nonkilling mechanism, i.e., avoids the use of antifungal actives and the emergence of funga... Read More about Fungal Attachment-Resistant Polymers for the Additive Manufacture of Medical Devices.

Computer Vision for Substrate Detection in High-Throughput Biomaterial Screens Using Bright-Field Microscopy (2024)
Journal Article
Owen, R., Nasir, A., H. Amer, M., Nie, C., Xue, X., Burroughs, L., Denning, C., D. Wildman, R., A. Khan, F., R. Alexander, M., & R. A. J. Rose, F. (2025). Computer Vision for Substrate Detection in High-Throughput Biomaterial Screens Using Bright-Field Microscopy. Advanced Intelligent Systems, 7(5), Article 2400573. https://doi.org/10.1002/aisy.202400573

High-throughput screening (HTS) can be used when ab initio information is unavailable for rational design of new materials, generating data on properties such as chemistry and topography that control cell behavior. Biomaterial screens are typically f... Read More about Computer Vision for Substrate Detection in High-Throughput Biomaterial Screens Using Bright-Field Microscopy.

Polymer-Coated Urinary Catheter Reduces Biofilm Formation and Biomineralization: A First-in-Man, Prospective Pilot Study (2024)
Journal Article
Kalenderski, K., Dubern, J.-F., Lewis-Lloyd, C., Jeffery, N., Heeb, S., Irvine, D. J., Sloan, T. J., Birch, B., Andrich, D., Humes, D., Alexander, M. R., & Williams, P. (2024). Polymer-Coated Urinary Catheter Reduces Biofilm Formation and Biomineralization: A First-in-Man, Prospective Pilot Study. Journal of Urology Open PLus, 2(1), Article e00005. https://doi.org/10.1097/JU9.0000000000000097

Purpose: Biofilm formation and biomineralization on urinary catheters may cause severe complications including infection and obstruction. Here, we describe an in vitro evaluation and prospective pilot clinical study of a silicone catheter coated with... Read More about Polymer-Coated Urinary Catheter Reduces Biofilm Formation and Biomineralization: A First-in-Man, Prospective Pilot Study.

A potential alternative to fungicides using actives-free (meth)acrylate polymers for protection of wheat crops from fungal attachment and infection (2023)
Journal Article
Crawford, L. A., Cuzzucoli Crucitti, V., Stimpson, A., Morgan, C., Blake, J., Wildman, R. D., Hook, A. L., Alexander, M. R., Irvine, D. J., & Avery, S. V. (2023). A potential alternative to fungicides using actives-free (meth)acrylate polymers for protection of wheat crops from fungal attachment and infection. Green Chemistry, 25(21), 8558-8569. https://doi.org/10.1039/d3gc01911j

Fungicidal compounds are actives widely used for crop protection from fungal infection, but they can also kill beneficial organisms, enter the food chain and promote resistant pathogen strains from overuse. Here we report the first field crop trial o... Read More about A potential alternative to fungicides using actives-free (meth)acrylate polymers for protection of wheat crops from fungal attachment and infection.

Discovery of a polymer resistant to bacterial biofilm, swarming, and encrustation (2023)
Journal Article
Dubern, J. F., Hook, A. L., Carabelli, A. M., Chang, C. Y., Lewis-Lloyd, C. A., Luckett, J. C., Burroughs, L., Dundas, A. A., Humes, D. J., Irvine, D. J., Alexander, M. R., & Williams, P. (2023). Discovery of a polymer resistant to bacterial biofilm, swarming, and encrustation. Science Advances, 9(4), Article eadd7474. https://doi.org/10.1126/sciadv.add7474

Innovative approaches to prevent catheter-associated urinary tract infections (CAUTIs) are urgently required. Here, we describe the discovery of an acrylate copolymer capable of resisting single- and multispecies bacterial biofilm formation, swarming... Read More about Discovery of a polymer resistant to bacterial biofilm, swarming, and encrustation.

Innate immune cell instruction using micron-scale 3D objects of varied architecture and polymer chemistry: The ChemoArchiChip (2023)
Journal Article
Vassey, M., Ma, L., Kämmerling, L., Mbadugha, C., Trindade, G. F., Figueredo, G. P., Pappalardo, F., Hutchinson, J., Markus, R., Rajani, S., Hu, Q., Winkler, D. A., Irvine, D. J., Hague, R., Ghaemmaghami, A. M., Wildman, R., & Alexander, M. R. (2023). Innate immune cell instruction using micron-scale 3D objects of varied architecture and polymer chemistry: The ChemoArchiChip. Matter, 6(3), 887-906. https://doi.org/10.1016/j.matt.2023.01.002

To design effective immunomodulatory implants, innate immune cell interactions at the surface of biomaterials need to be controlled and understood. The architectural design freedom of two-photon polymerization is used to produce arrays of surface-mou... Read More about Innate immune cell instruction using micron-scale 3D objects of varied architecture and polymer chemistry: The ChemoArchiChip.

Predictive Molecular Design and Structure–Property Validation of Novel Terpene-Based, Sustainably Sourced Bacterial Biofilm-Resistant Materials (2023)
Journal Article
Cuzzucoli Crucitti, V., Ilchev, A., Moore, J. C., Fowler, H. R., Dubern, J.-F., Sanni, O., Xue, X., Husband, B. K., Dundas, A. A., Smith, S., Wildman, J. L., Taresco, V., Williams, P., Alexander, M. R., Howdle, S. M., Wildman, R. D., Stockman, R. A., & Irvine, D. J. (2023). Predictive Molecular Design and Structure–Property Validation of Novel Terpene-Based, Sustainably Sourced Bacterial Biofilm-Resistant Materials. Biomacromolecules, 24(2), 576-591. https://doi.org/10.1021/acs.biomac.2c00721

Presented in this work is the use of a molecular descriptor, termed the α parameter, to aid in the design of a series of novel, terpene-based, and sustainable polymers that were resistant to biofilm formation by the model bacterial pathogen Pseudomon... Read More about Predictive Molecular Design and Structure–Property Validation of Novel Terpene-Based, Sustainably Sourced Bacterial Biofilm-Resistant Materials.

Microparticles Decorated with Cell‐Instructive Surface Chemistries Actively Promote Wound Healing (2022)
Journal Article
Latif, A., Fisher, L. E., Dundas, A. A., Crucitti, V. C., Imir, Z., Lawler, K., Pappalard, F., Muir, B. W., Wildman, R., Irvine, D. J., Alexander, M. R., & Ghaemmaghami, A. M. (2024). Microparticles Decorated with Cell‐Instructive Surface Chemistries Actively Promote Wound Healing. Advanced Materials, 36(43), Article 2208364. https://doi.org/10.1002/adma.202208364

Wound healing is a complex biological process involving close crosstalk between various cell types. Dysregulation in any of these processes, such as in diabetic wounds, results in chronic nonhealing wounds. Fibroblasts are a critical cell type involv... Read More about Microparticles Decorated with Cell‐Instructive Surface Chemistries Actively Promote Wound Healing.

Feature importance in machine learning models: A fuzzy information fusion approach (2022)
Journal Article
Rengasamy, D., Mase, J. M., Kumar, A., Rothwell, B., Torres, M. T., Alexander, M. R., Winkler, D. A., & Figueredo, G. P. (2022). Feature importance in machine learning models: A fuzzy information fusion approach. Neurocomputing, 511, 163-174. https://doi.org/10.1016/j.neucom.2022.09.053

With the widespread use of machine learning to support decision-making, it is increasingly important to verify and understand the reasons why a particular output is produced. Although post-training feature importance approaches assist this interpreta... Read More about Feature importance in machine learning models: A fuzzy information fusion approach.

Exploiting Generative Design for Multi-Material Inkjet 3D Printed Cell Instructive, Bacterial Biofilm Resistant Composites (2022)
Preprint / Working Paper
he, Y., Begines, B., Trindade, G., Abdi, M., dubern, J.-F., Prina, E., Hook, A., Choong, G., Ledesma, J., Tuck, C., R. A. J. Rose, F., Hague, R., Roberts, C., De Focatiis, D., Ashcroft, I., Williams, P., Irvine, D., alexander, M., & Wildman, R. Exploiting Generative Design for Multi-Material Inkjet 3D Printed Cell Instructive, Bacterial Biofilm Resistant Composites

As our understanding of disease grows, it is becoming established that treatment needs to be personalized and targeted to the needs of the individual. In this paper we show that multi-material inkjet-based 3D printing, when backed with generative des... Read More about Exploiting Generative Design for Multi-Material Inkjet 3D Printed Cell Instructive, Bacterial Biofilm Resistant Composites.

Utilising micron scale 3D printed morphologies for particle adhesion reduction (2022)
Journal Article
Marsh, G. E., Bunker, M. J., Alexander, M. R., Wildman, R. D., Nicholas, M., & Roberts, C. J. (2022). Utilising micron scale 3D printed morphologies for particle adhesion reduction. Powder Technology, 404, Article 117418. https://doi.org/10.1016/j.powtec.2022.117418

In the pharmaceutical industry, the ability to improve the understanding of the effect of surface roughness on interparticulate interactions is critical. Dry powder inhalers often possess poor efficiency, as the powder formulations are inherently adh... Read More about Utilising micron scale 3D printed morphologies for particle adhesion reduction.

Molecular Formula Prediction for Chemical Filtering of 3D OrbiSIMS Datasets (2022)
Journal Article
Edney, M. K., Kotowska, A. M., Spanu, M., Trindade, G. F., Wilmot, E., Reid, J., Barker, J., Aylott, J. W., Shard, A. G., Alexander, M. R., Snape, C. E., & Scurr, D. J. (2022). Molecular Formula Prediction for Chemical Filtering of 3D OrbiSIMS Datasets. Analytical Chemistry, 94(11), 4703–4711. https://doi.org/10.1021/acs.analchem.1c04898

Modern mass spectrometry techniques produce a wealth of spectral data, and although this is an advantage in terms of the richness of the information available, the volume and complexity of data can prevent a thorough interpretation to reach useful co... Read More about Molecular Formula Prediction for Chemical Filtering of 3D OrbiSIMS Datasets.

Correction to “Bespoke 3D-Printed Polydrug Implants Created via Microstructural Control of Oligomers” (2022)
Journal Article
Ruiz-Cantu, L., Trindade, G. F., Taresco, V., Zhou, Z., He, Y., Burroughs, L., Clark, E. A., Rose, F. R. A. J., Tuck, C., Hague, R., Roberts, C. J., Alexander, M., Irvine, D. J., & Wildman, R. D. (2022). Correction to “Bespoke 3D-Printed Polydrug Implants Created via Microstructural Control of Oligomers”. ACS Applied Materials and Interfaces, 14(6), 8654. https://doi.org/10.1021/acsami.2c00035

The chemical structure of the drug trandolapril has been corrected in Figure 4c. The conclusions of the work have not been affected by this correction. (Figure present).

Ink-jet 3D printing as a strategy for developing bespoke non-eluting biofilm resistant medical devices (2021)
Journal Article
He, Y., Luckett, J., Begines, B., Dubern, J. F., Hook, A. L., Prina, E., Rose, F. R., Tuck, C. J., Hague, R. J., Irvine, D. J., Williams, P., Alexander, M. R., & Wildman, R. D. (2022). Ink-jet 3D printing as a strategy for developing bespoke non-eluting biofilm resistant medical devices. Biomaterials, 281, Article 121350. https://doi.org/10.1016/j.biomaterials.2021.121350

Chronic infection as a result of bacterial biofilm formation on implanted medical devices is a major global healthcare problem requiring new biocompatible, biofilm-resistant materials. Here we demonstrate how bespoke devices can be manufactured throu... Read More about Ink-jet 3D printing as a strategy for developing bespoke non-eluting biofilm resistant medical devices.

Customisable Tablet Printing: The Development of Multimaterial Hot Melt Inkjet 3D Printing to Produce Complex and Personalised Dosage Forms (2021)
Journal Article
Lion, A., Wildman, R. D., Alexander, M. R., & Roberts, C. J. (2021). Customisable Tablet Printing: The Development of Multimaterial Hot Melt Inkjet 3D Printing to Produce Complex and Personalised Dosage Forms. Pharmaceutics, 13(10), Article 1679. https://doi.org/10.3390/pharmaceutics13101679

One of the most striking characteristics of 3D printing is its capability to produce multi-material objects with complex geometry. In pharmaceutics this translates to the possibility of dosage forms with multi-drug loading, tailored dosing and releas... Read More about Customisable Tablet Printing: The Development of Multimaterial Hot Melt Inkjet 3D Printing to Produce Complex and Personalised Dosage Forms.

A new particle mounting method for surface analysis (2021)
Journal Article
Dundas, A. A., Kern, S., Cuzzucoli Crucitti, V., Scurr, D. J., Wildman, R., Irvine, D. J., & Alexander, M. R. (2022). A new particle mounting method for surface analysis. Surface and Interface Analysis, 54(4), 374-380. https://doi.org/10.1002/sia.7010

The chemical analysis of microparticles is challenging due to the need to mount the particles on a substrate for analysis; double-sided adhesive tape is often used (sometimes conductive), however that is usually coated with poly (dimethyl siloxane) (... Read More about A new particle mounting method for surface analysis.